Difference between revisions of "SUIT-002 O2 mt D005"

Revision as of 14:06, 13 June 2018

high-resolution terminology - matching measurements at high-resolution

SUIT-002 O2 mt D005

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT 2 is the RP2 - MiPNet21.06 SUIT RP

SUIT number: 2

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP2

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: diametral

Harmonized SUIT protocols

RP1 and RP2

SUIT RP1 SUIT RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd

SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2

RP1 and RP2 in cells

RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway statea.

SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd

Harmonization between RP1 and RP2 in cells

RP1 and RP2 in PBMCs and PTLs

In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs

SUIT RP1 in PBMCs and PTLsSUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd

SUIT RP2 PBMCs and PLTs: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2 in PBMC and PTL cells

SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3)

Updating this page (2018-06-13) for DatLab 7.3 DLP files

References

@@ Line 17: / Line 17: @@
 == Harmonized SUIT protocols ==
+=== RP1 and RP2 ===
 '''SUIT RP1''' [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]]
 '''SUIT RP2''' [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]]
@@ Line 25: / Line 28: @@
 [[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2'''
+=== RP1 and RP2 in cells ===
 RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Electron transfer-pathway state|Electron transfer-pathway statea]].
@@ Line 33: / Line 37: @@
+=== RP1 and RP2 in PBMCs and PTLs ===
+In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: [[1PM;2D;3U;4G;5S;6Rot;7Gp-|SUIT 1 for PBMCs and PTLs]]
-== SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.1) ==
+:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in PBMCs and PTLs]]'''SUIT RP1 for PBMCs and PLTs''':1PM;2D;2c;3U;4G;5S;6Rot;'''7Gp''';8Ama;'''9AsTm''';10Azd
+:::: '''SUIT RP2 PBMCs and PLTs''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd
-:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and Electron transfer-pathway states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols [[MitoPedia: SUIT]]. Instrumental DL-protocols are displayed in the [[DL-Protocols#DL-Protocol_library| DL-Protocol library]].
-{{Template:Download instructions}}
-{| class="wikitable" border="1"
-|-
-! DL-Protocol
-! Description
-! Download - Protocol .DLP
-! Download - Analysis .XLSX
-|-
-| SUIT 2_pce
-| SUIT reference protocol 2 (RP2) for permeabilized cells (pce) with digitonin
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/1/14/RP2pce%28Dig%29.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/c/ca/RP2pce%28Dig%29.xlsx]]
-|-
+[[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells'''
-| SUIT 2_mt
-| SUIT reference protocol RP2 for mt-preparations: isolated mitochondria (imt) and tissue homogenate (thom)
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/5/53/RP2mt.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/9/9e/RP2mt.xlsx]]
-|-
-| SUIT 2_pfi
-| SUIT reference protocol RP2 for permeabilized fibers (pfi)
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/d/d1/RP2pfi.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/5/5b/RP2pfi.xlsx]]
-|-
+== SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3) ==
-| SUIT 2_pce_PBMC_PLT
-| SUIT reference protocol RP2 for PBMC and PLT
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/2/2e/RP2pce%28Dig%29_PBMC_PLT.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/6/6e/RP2pce%28Dig%29_PBMC_PLT.xlsx]]
-|}
+''Updating this page (2018-06-13) for DatLab 7.3 DLP files''