Difference between revisions of "SUIT-002 O2 mt D005"

Revision as of 15:34, 18 April 2018

high-resolution terminology - matching measurements at high-resolution

SUIT-002 O2 mt D005

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT 2 is the RP2 - MiPNet21.06 SUIT RP

SUIT number: 2

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP2

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: diametral

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd

SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2

RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway statea.

SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd

Harmonization between RP1 and RP2 in cells

SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.1)

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and Electron transfer-pathway states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.

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DL-Protocol	Description	Download - Protocol .DLP	Download - Analysis .XLSX
SUIT 2_pce	SUIT reference protocol 2 (RP2) for permeabilized cells (pce) with digitonin
SUIT 2_mt	SUIT reference protocol RP2 for mt-preparations: isolated mitochondria (imt) and tissue homogenate (thom)
SUIT 2_pfi	SUIT reference protocol RP2 for permeabilized fibers (pfi)
SUIT 2_pce_PBMC_PLT	SUIT reference protocol RP2 for PBMC and PLT

References

@@ Line 25: / Line 25: @@
 [[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2'''
-RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Pathway control state|pathway control states]].
+RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Electron transfer-pathway state|Electron transfer-pathway statea]].
 :::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in cells]]: '''ce1''';1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd
@@ Line 36: / Line 36: @@
 == SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.1) ==
-:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols [[MitoPedia: SUIT]]. Instrumental DL-protocols are displayed in the [[DL-Protocols#DL-Protocol_library| DL-Protocol library]].
+:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and Electron transfer-pathway states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols [[MitoPedia: SUIT]]. Instrumental DL-protocols are displayed in the [[DL-Protocols#DL-Protocol_library| DL-Protocol library]].
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