Difference between revisions of "SUIT-002 O2 mt D005"
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=FNSGp(PGM) | |abbr=FNSGp(PGM) | ||
|description=[[File:1D; | |description=[[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]] | ||
|info='''A: SUIT_RP2''' - [[MiPNet21.06 SUIT RP]] | |info='''A: SUIT_RP2''' - [[MiPNet21.06 SUIT RP]] | ||
}} | }} | ||
Line 16: | Line 16: | ||
== Harmonized SUIT protocols == | == Harmonized SUIT protocols == | ||
[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama; | [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]] '''SUIT RP1''' | ||
[[File:1D; | [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px|SUIT-RP2]] '''SUIT RP2''' | ||
:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1]]: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10AsTm''';11Azd | :::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1]]: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10AsTm''';11Azd | ||
:::: '''SUIT RP2''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd | :::: '''SUIT RP2''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd | ||
[[File: | [[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2''' | ||
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Pathway control state|pathway control states]]. | RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Pathway control state|pathway control states]]. | ||
Line 29: | Line 29: | ||
:::: '''SUIT RP2 in cells''': '''ce1''';1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azdd | :::: '''SUIT RP2 in cells''': '''ce1''';1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azdd | ||
[[File: | [[File:RP1&RP2_pce(Dig).png|400px]] '''Harmonization between RP1 and RP2 in cells''' | ||
Revision as of 16:26, 27 November 2017
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
- SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet pathway control states.
- SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
SUIT O2 DLP for RP2 (DatLab 7.1)
- DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.
Download instructions.
To start the download click on the icon. Depending on your browser you will encounter different download scenarios.
(i) A window opens to select whether to "open file with" or to "save file". Please always choose the option "save file"; opening the file directly will not work due to safety reasons.
(ii) When you click the download icon, some browsers will display the text/content of the .DLP file instead of downloading it. In this case go back to this download page and right-click the download icon and choose "save linked file as" from the context-menu. A window will open to save the file. In some cases the suffix of the file might be wrong (e.g. ".HTML") - you must correct the suffix of the file to ".DLP".