Difference between revisions of "SUIT-002 O2 mt D005"

Revision as of 10:43, 8 August 2017

high-resolution terminology - matching measurements at high-resolution

SUIT-002 O2 mt D005

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP2

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: diametral

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2: 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

Harmonization between RP1 and RP2

DL-Protocols for SUIT protocol RP2 (DatLab 7.1)

DL-Protocols for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both.

DL-Protocol	Sample	DLP File
DL-Protocol	Sample	O₂	O₂&Amp (H₂O₂ production)
RP2	mt-preparations	In progress	In progress
RP2	Permeabiliced cells (pce) with digitonin	RP1pce(Dig)	In progress
RP2	Permeabilized cells (pce) without digitonin	In progress	In progress
RP2	Permeabilized fibers	In progress	In progress

A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayed in DL-Protocols.

DatLab-Analysis templates for RP1 analysis (DatLab 7.1)

SUIT Reference protocol	Sample	O2k-Core
RP2	mt-preparations	»SUIT RP2mt
RP2	Permeabilized cells (pce) with digitonin	»SUIT RP2pce(Dig)
RP2	Permeabilized cells (pce) without digitonin	»SUIT RP2pce
RP2	Permeabilized fibers	»SUIT RP2mt	-

References

@@ Line 27: / Line 27: @@
 [[File:SUIT-Reference-Protocol.jpg|400px]] '''Harmonization between RP1 and RP2'''
-== DL-Drivers for SUIT protocol RP2 ==
+== DL-Protocols for SUIT protocol RP2 (DatLab 7.1)==
-{| class="wikitable" border="1"
+[[DL-Protocols]] for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) proceed stepwise to activate a sequence of coupling control states and pathway control states. A specific SUIT protocol can be assigned to O2k-chamber A or B or both.
+{| class="wikitable" border="1"
 |-
-! rowspan="2" style=text-align:center" | SUIT Reference protocol
+! rowspan="2" style=text-align:center" | DL-Protocol
 ! rowspan="2" style=text-align:center" | Sample
-! colspan="2" style=text-align:center" | O2k-Core
+! colspan="2" style=text-align:center" | DLP File
-! colspan="2" style=text-align:center" | O2k-Amp (H<sub>2</sub>O<sub>2</sub> production)
 |-
-| '''Lab sheets'''
+| style="text-align:center" | '''O<sub>2</sub>'''
-| '''DL-Drivers version 7.1 '''
+| style="text-align:center" |'''O<sub>2</sub>&Amp (H<sub>2</sub>O<sub>2</sub> production)'''
-| '''Lab sheets'''
-| '''DL-Drivers version 7.1'''
 |-
 | RP2
 | mt-preparations
-| [[Media:RP2mt.pdf |»SUIT RP2mt]]
 | ''In progress''
-| [[Media:RP2mt_AmR.pdf |»SUIT RP2mt_AmR]]
+| ''In progress''
-|  ''In progress''
 |-
 | RP2
-| Permeabilized cells (pce) with digitonin
+| Permeabiliced cells (pce) with digitonin
-| [[Media:RP2pce(Dig).pdf |»SUIT RP2pce(Dig)]]
+| [[Media:RP1PCE(DIG).DLP|RP1pce(Dig)]]
-|  ''In progress''
+| ''In progress''
-| [[Media:RP2pce(Dig)_AmR.pdf |»SUIT RP2pce(Dig)_AmR]]
-|  ''In progress''
 |-
 | RP2
 | Permeabilized cells (pce) without digitonin
-| [[Media:RP2pce.pdf |»SUIT RP2pce]]
+| ''In progress''
-|  ''In progress''
+| ''In progress''
-| [[Media:RP2pce_AmR.pdf |»SUIT RP2pce_AmR]]
-|  ''In progress''
 |-
 | RP2
 | Permeabilized fibers
-| [[Media:RP2pfi.pdf |»SUIT RP2pfi]]
+| ''In progress''
-|  ''In progress''
+| ''In progress''
-|   -
+|-
-|  ''In progress''
+|}
-|}
+A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayed  in [[DL-Protocols]].
-== DatLab-Analysis templates for RP2 analysis ==
+== DatLab-Analysis templates for RP1 analysis (DatLab 7.1) ==
@@ Line 83: / Line 77: @@
 ! Sample
 ! O2k-Core
-! O2k-Amp (H<sub>2</sub>O<sub>2</sub> production)
 |-
@@ Line 89: / Line 82: @@
 | mt-preparations
 | [[Media:RP2mt.xlsx |»SUIT RP2mt]]
-| [[Media:RP2mt_AmR.xlsx |»SUIT RP2mt_AmR]]
 |-
-| RP1
+| RP2
 | Permeabilized cells (pce) with digitonin
 | [[Media:RP2pce(Dig).xlsx |»SUIT RP2pce(Dig)]]
-|  -
 |-
-| RP1
+| RP2
 | Permeabilized cells (pce) without digitonin
 | [[Media:RP2pce.xlsx |»SUIT RP2pce]]
-|  -
 |-
-| RP1
+| RP2
 | Permeabilized fibers
 | [[Media:RP2mt.xlsx |»SUIT RP2mt]]