Difference between revisions of "SUIT-002 O2 mt D005"

Revision as of 17:29, 14 March 2017

high-resolution terminology - matching measurements at high-resolution

SUIT-002 O2 mt D005

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT_RP2 - MiPNet21.06 SUIT RP

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP2

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: diametral

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2: 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

Laboratory sheets for SUIT protocol RP2

SUIT Reference protocol	Sample	Respiration	H₂O₂ production
RP2	mt-preparations	»SUIT RP2mt;	»SUIT RP2mt_AmR
RP2	Permeabilized cells (pce) with digitonin	»SUIT RP2pce(Dig)	»SUIT RP2pce(Dig)_AmR
RP2	Permeabilized cells (pce) without digitonin	»SUIT RP2pce	»SUIT RP2pce_AmR
RP2	Permeabilized fibers	»SUIT RP2pfi

DatLab-Analysis templates for RP2 analysis

SUIT Reference protocol	Sample	Respiration (DatLab7)	H₂O₂ production (DatLab6)
RP1	mt-preparations	Excell SHEET	Excell SHEET H2O2
RP1	Permeabilized cells (pce) with digitonin	Excell SHEET	Excell SHEET H2O2
RP1	Permeabilized cells (pce) without digitonin	Excell SHEET	Excell SHEET H2O2
RP1	Permeabilized fibers	Excell SHEET	Excell SHEET H2O2
RP2	mt-preparations	Excell SHEET	Excell SHEET H2O2
RP2	Permeabilized cells (pce) with digitonin	Excell SHEET	Excell SHEET H2O2
RP2	Permeabilized cells (pce) without digitonin	Excell SHEET	Excell SHEET H2O2
RP2	Permeabilized fibers	Excell SHEET	Excell SHEET H2O2

Mark names in DatLab: RP2mt, RP2pce, RP2pfi

Laboratory titration sheet: »SUIT RP2mt; »SUIT RP2mt_AmR

Laboratory titration sheet: »SUIT RP2pce(Dig); »SUIT RP2pce(Dig)_AmR

Laboratory titration sheet: »SUIT RP2pce; »SUIT RP2pce_AmR

Laboratory titration sheet: »SUIT RP2pfi

DatLab-Analysis template: SUIT_MiPNet21.06_RP1_RP2mt.xlsx, SUIT_MiPNet21.06_RP1_RP2pce.xlsx, SUIT_MiPNet21.06_RP1_RP2pfi.xlsx

References

RP2 current version

1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot-

RP2 Version Test02

1D;2Oct;2M.1;2M2;2c;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

@@ Line 1: / Line 1: @@
+__TOC__
 {{MitoPedia
 |abbr=FNSGp(PGM)
@@ Line 10: / Line 11: @@
 ::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNSGp(PGM)
 ::: '''[[SUIT protocol pattern]]:''' diametral
+== Harmonized SUIT protocols ==
+[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd.jpg|400px|SUIT-RP1]] '''SUIT RP1'''
+[[File:1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd.jpg|400px|SUIT-RP2]] '''SUIT RP2'''
+:::: [[SUIT RP1]]: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
+:::: '''SUIT RP2''': 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd
+== Laboratory sheets for SUIT protocol RP2 ==
+{| class="wikitable" border="1"
+|-
+! SUIT Reference protocol
+! Sample
+! Respiration
+! H<sub>2</sub>O<sub>2</sub> production
+|-
+| RP2
+| mt-preparations
+| [[Media:RP2mt.pdf |»SUIT RP2mt]];
+| [[Media:RP2mt_AmR.pdf |»SUIT RP2mt_AmR]]
+|-
+| RP2
+| Permeabilized cells (pce) with digitonin
+| [[Media:RP2pce(Dig).pdf |»SUIT RP2pce(Dig)]]
+| [[Media:RP2pce(Dig)_AmR.pdf |»SUIT RP2pce(Dig)_AmR]]
+|-
+| RP2
+| Permeabilized cells (pce) without digitonin
+| [[Media:RP2pce.pdf |»SUIT RP2pce]]
+| [[Media:RP2pce_AmR.pdf |»SUIT RP2pce_AmR]]
+|-
+| RP2
+| Permeabilized fibers
+| [[Media:RP2pfi.pdf |»SUIT RP2pfi]]
+|}
+== DatLab-Analysis templates for RP2 analysis ==
+{| class="wikitable" border="1"
+|-
+! SUIT Reference protocol
+! Sample
+! Respiration (DatLab7)
+! H<sub>2</sub>O<sub>2</sub> production (DatLab6)
+|-
+| RP1
+| mt-preparations
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP1
+| Permeabilized cells (pce) with digitonin
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP1
+| Permeabilized cells (pce) without digitonin
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP1
+| Permeabilized fibers
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP2
+| mt-preparations
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP2
+| Permeabilized cells (pce) with digitonin
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP2
+| Permeabilized cells (pce) without digitonin
+| Excell SHEET
+| Excell SHEET H2O2
+|-
+| RP2
+| Permeabilized fibers
+| Excell SHEET
+| Excell SHEET H2O2
+|}
 ::: '''[[Mark names in DatLab]]:''' RP2mt, RP2pce, RP2pfi
 ::: '''[[Laboratory titration sheet]]:''' [[Media:RP2mt.pdf |»SUIT RP2mt]]; [[Media:RP2mt_AmR.pdf |»SUIT RP2mt_AmR]]
@@ Line 46: / Line 155: @@
 <br />
 <br />
-== Harmonized SUIT protocols ==
-[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd.jpg|400px|SUIT-RP1]] '''SUIT RP1'''
-[[File:1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd.jpg|400px|SUIT-RP2]] '''SUIT RP2'''
-:::: [[SUIT RP1]]: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
-:::: '''SUIT RP2''': 1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd