Difference between revisions of "SUIT-002"
From Bioblast
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::: '''[[SUIT protocol pattern]]:''' diametral 1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama | ::: '''[[SUIT protocol pattern]]:''' diametral 1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama | ||
__TOC__ | __TOC__ | ||
Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-01-22) | |||
== DLP applications == | == DLP applications == |
Revision as of 14:18, 22 January 2019
Description
Abbreviation: FNSGp(Oct,PGM)
Reference: A: RP2 Doerrier 2018 Methods Mol Biol »Versions
- SUIT-category: FNSGp(Oct,PGM)
- SUIT protocol pattern: diametral 1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama
Communicated by Doerrier C and Gnaiger E (last update 2019-01-22)
DLP applications
- SUIT-002 O2 mt D005 for isolated mitochondria and tissue homogenate
- SUIT-002 O2 pfi D006 for permeabilized fibers
- SUIT-002 O2 pce D007 for permeabilized cells
- SUIT-002 O2 pce D007a for permeabilized PBMCs and PLTs (absense of Oct)
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1D | REN | 1D
| ||
2M.1 | 1D;2M.1 | |||
3Oct | OctMP | F | FAO | 1D;2M.1;3Oct
|
3c | OctMcP | F | FAO | 1D;2M.1;3Oct;3c
|
4M2 | OctMP | F(N) | FAO | 1D;2M.1;3Oct;3c;4M2
|
5P | OctPMP | FN | FAO&CI | 1D;2M.1;3Oct;4M2;5P
|
6G | OctPGMP | FN | FAO&CI | 1D;2M.1;3Oct;4M2;5P;6G
|
7S | OctPGMSP | FNS | FAO&CI&II | 1D;2M.1;3Oct;4M2;5P;6G;7S
|
8Gp | OctPGMSGpP | FNSGp | FAO&CI&II&GpDH | 1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp
|
9U | OctPGMSGpE | FNSGp | FAO&CI&II&GpDH | 1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U
|
10Rot | SGpE | SGp | CII&GpDH | 1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot
|
11Ama | ROX | 1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
Compare SUIT protocols
- SUIT-001 (RP1): Harmonized SUIT protocol.
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry