Difference between revisions of "Piwonska 2016 Acta Neurobiol Exp (Wars)"
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{{Publication | {{Publication | ||
|title=Piwońska M, Szewczyk A, Schröder UH, Reymann KG, Bednarczyk | |title=Piwońska M, Szewczyk A, Schröder UH, Reymann KG, Bednarczyk P (2016) Effectors of large-conductance calcium-activated potassium channel modulate glutamate excitotoxicity in organotypic hippocampal slice cultures. Acta Neurobiol Exp (Wars) 76:20-31. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/27102915 PMID: 27102915 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/27102915 PMID: 27102915 Open Access] | ||
|authors=Piwonska M, Szewczyk A, Schroeder UH, Reymann KG, Bednarczyk | |authors=Piwonska M, Szewczyk A, Schroeder UH, Reymann KG, Bednarczyk P | ||
|year=2016 | |year=2016 | ||
|journal=Acta Neurobiol Exp (Wars) | |journal=Acta Neurobiol Exp (Wars) |
Revision as of 14:54, 22 June 2016
Piwońska M, Szewczyk A, Schröder UH, Reymann KG, Bednarczyk P (2016) Effectors of large-conductance calcium-activated potassium channel modulate glutamate excitotoxicity in organotypic hippocampal slice cultures. Acta Neurobiol Exp (Wars) 76:20-31. |
Piwonska M, Szewczyk A, Schroeder UH, Reymann KG, Bednarczyk P (2016) Acta Neurobiol Exp (Wars)
Abstract: Mitochondria have been suggested as a potential target for cytoprotective strategies. It has been shown that increased K+ uptake mediate by mitochondrial ATP-regulated potassium channels (mitoKATP channel) or large-conductance Ca2+-activated potassium channels (mitoBKCa channel) may provide protection in different models of cell death. Since recent findings demonstrated the presence of BKCa channels in neuronal mitochondria, the goal of the present study was to test the potential neuroprotective effects of BKCa channel modulators. Using organotypic hippocampal slice cultures exposed to glutamate, we demonstrated that preincubation of the slices with the BKCa channel opener NS1619 resulted in decreased neuronal cell death measured as reduced uptake of propidium iodide. This neuroprotective effect was reversed by preincubation with the BKCa channel inhibitors paxilline and Iberiotoxin (IbTx). Moreover, mitochondrial respiration measurements revealed that NS1619 induced an IbTx-sensitive increase in state 2 respiration of isolated brain mitochondria. In addition, electrophysiological patch-clamp studies confirmed the presence of BKCa channels in mitoplasts isolated from embryonic hippocampal cells. Taken together, our results confirm presence of BKCa channel in rat hippocampal neurons mitochondria and suggest putative role for mitoBKCa in neuroprotection. • Keywords: Potassium channel, Glutamate, Hippocampal slice cultures, Mitochondria, Respiration, Patch-clamp
• O2k-Network Lab: PL Warsaw Bednarczyk P, PL Warsaw Szewczyk A
Labels: MiParea: Respiration
Stress:Cell death Organism: Human, Rat Tissue;cell: Nervous system Preparation: Isolated mitochondria
Coupling state: OXPHOS
HRR: Oxygraph-2k
Labels, 2016-06