Difference between revisions of "Pino 2017 Int J Obes (Lond)"
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{{Publication | {{Publication | ||
|title=Pino MF, Divoux A, Simmonds AV, Smith SR, Sparks LM (2017) Investigating the effects of Orexin-A on thermogenesis in human deep neck brown adipose tissue. Int J Obes (Lond) | |title=Pino MF, Divoux A, Simmonds AV, Smith SR, Sparks LM (2017) Investigating the effects of Orexin-A on thermogenesis in human deep neck brown adipose tissue. Int J Obes (Lond) 41:1646-53. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/28674442 PMID: 28674442] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/28674442 PMID: 28674442] | ||
|authors=Pino MF, Divoux A, Simmonds AV, Smith SR, Sparks LM | |authors=Pino MF, Divoux A, Simmonds AV, Smith SR, Sparks LM | ||
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{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Pharmacology;toxicology | ||
|diseases=Obesity | |diseases=Obesity | ||
|organism=Human | |organism=Human | ||
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|couplingstates=LEAK, ROUTINE | |couplingstates=LEAK, ROUTINE | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2017-07, | ||
}} | }} |
Latest revision as of 10:14, 26 June 2019
Pino MF, Divoux A, Simmonds AV, Smith SR, Sparks LM (2017) Investigating the effects of Orexin-A on thermogenesis in human deep neck brown adipose tissue. Int J Obes (Lond) 41:1646-53. |
Pino MF, Divoux A, Simmonds AV, Smith SR, Sparks LM (2017) Int J Obes (Lond)
Abstract: Despite successful pre-clinical testing, 85% of early clinical trials for novel drugs fail. Most futilities originate from molecular mechanisms of the drug(s) tested. It is critically important to develop validated human cell-based model systems in which animal-based research can be translated in order to complement the preclinical in vivo findings prior to implementation of a clinical trial. Obesity is associated with reduced circulating levels of Orexin-A (OX-A) in humans. OX-A increases thermogenesis in rodent brown adipose tissue (BAT), yet this phenomenon has not been explored in humans.
We established a cell-based model system of human brown and white adipocytes and tested the effects of OX-A on thermogenesis.
Contrary to published in vivo and in vitro reports in rodents, OX-A treatment alone or in combination with an adrenergic stimulus did not enhance thermogenesis nor its related transcriptional program in a human in vitro model of brown adipocytes nor adipose tissue explants.
Translating preclinical findings in human model systems poses a challenge that must be overcome for the development of effective therapeutic compounds and targets.
β’ Bioblast editor: Kandolf G β’ O2k-Network Lab: US FL Orlando Sparks LM, US FL Orlando Translational Research Institute
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Obesity
Organism: Human Tissue;cell: Fat Preparation: Intact cells
Coupling state: LEAK, ROUTINE
HRR: Oxygraph-2k
2017-07