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Difference between revisions of "Pham 2014 Am J Physiol"

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{{Publication
{{Publication
|title=Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.
|title=Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/24920675 PMID: 24920675]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/24920675 PMID: 24920675 Open Access]
|authors=Pham T, Loiselle D, Power A, Hickey AJ
|authors=Pham T, Loiselle D, Power A, Hickey AJ
|year=2014
|year=2014
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|mipnetlab=NZ Auckland Hickey AJ
|mipnetlab=NZ Auckland Hickey AJ
}}
}}
Demonstration of using Amplex Red, Safranin and Magnesium green methods with the O2k-Fluorescence Module.
== Correction ==
An Oroboros Oxygraph-2k was used in this publication, whereas the Anton Paar/Oroboros Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the Oxygraph-2k in 2002.
* ''Further details'': [[Gnaiger 2012 Abstract Bioblast-Gentle Science]]
== Cited by ==
{{Template:Cited by Cardoso 2021 MitoFit MgG}}
{{Labeling
{{Labeling
|area=Respiration, mt-Medicine
|area=Respiration, mt-Medicine
|diseases=Diabetes, Myopathy
|injuries=Ischemia-reperfusion, Oxidative stress;RONS, Mitochondrial disease
|organism=Rat
|organism=Rat
|tissues=Heart
|tissues=Heart
|preparations=Homogenate
|preparations=Homogenate
|injuries=Ischemia-reperfusion, Oxidative stress;RONS, Mitochondrial disease
|diseases=Diabetes, Myopathy
|topics=Coupling efficiency;uncoupling, mt-Membrane potential
|topics=Coupling efficiency;uncoupling, mt-Membrane potential
|couplingstates=LEAK, OXPHOS, ETS
|couplingstates=LEAK, OXPHOS, ET
|substratestates=CI, CI&II, ROX
|pathways=N, NS, ROX
|instruments=Oxygraph-2k, O2k-Fluorometer
|instruments=Oxygraph-2k, O2k-Fluorometer
|additional=Amplex Red, Safranin, Magnesium Green
|additional=Safranin, MgG, AmR, MitoFit 2021 MgG
}}
}}
Demonstration of using Amplex Red, Safranin and Magnesium green methods with the O2k-Fluoresececen Module.
== Correction ==
An OROBOROS Oxygraph-2k was used in this publication, whereas the Anton Paar/OROBOROS Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the Oxygraph-2k in 2002.
* ''Further details'': [[Gnaiger 2012 Abstract Bioblast-Gentle Science]]

Revision as of 15:47, 25 January 2021

Publications in the MiPMap
Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.

Β» PMID: 24920675 Open Access

Pham T, Loiselle D, Power A, Hickey AJ (2014) Am J Physiol

Abstract: As approximately 80% of diabetics die from heart failure, understanding diabetic cardiomyopathy is crucial. Mitochondria occupy 35-40% of mammalian cardiomyocyte volume, supply 95% of the hearts' ATP, and diabetic heart mitochondria show impaired structure, arrangement and function. We predict that bioenergetic efficiencies are present in diabetic heart mitochondria; therefore we explored mitochondrial proton and electron handling by linking oxygen flux within streptozotocin (STZ)-induced-diabetic Sprague-Dawley rat heart tissues, to steady-state ATP synthesis, Reactive Oxygen Species (ROS) production, and mitochondrial membrane potential (ΔΨ). By coupling high-resolution respirometers with purpose-built fluorometers, we followed Magnesium Green (ATP synthesis), Amplex Ultra Red (ROS production), and safranin-O (ΔΨ). Relative to control rats, the mass-specific respiration of STZ-diabetic hearts was depressed in oxidative phosphorylating (OXPHOS) states. Steady-state ATP synthesis capacity was almost a third lower in STZ-diabetic heart and relative to O2 flux, this equates to an estimated 12% depression in OXPHOS efficiency. However, with anoxic transition, STZ-diabetic and control heart tissues showed similar ATP hydrolysis capacities through reversal of the F1/F0 ATP-synthase. STZ-diabetic cardiac mitochondria also produced more net ROS relative to oxygen flux (ROS/O) in OXP. While ΔΨ did not differ between groups, the time to develop ΔΨ with the onset of OXPHOS was protracted in STZ-diabetic mitochondria. ROS/O is higher in life-like OXPHOS states and potential delays in the time to develop ΔΨ may delay ATP synthesis with inter-beat fluctuations in ADP concentrations. Whereas diabetic cardiac mitochondria produce less ATP in normoxia, they consume as much ATP in anoxic infarct-like states. β€’ Keywords: Diabetic cardiomypathy, Anoxia, Efficiency, Mitochondria, Oxidative phosphorylation, Safranin, Amplex Red, Magnesium Green

β€’ O2k-Network Lab: NZ Auckland Hickey AJ


Demonstration of using Amplex Red, Safranin and Magnesium green methods with the O2k-Fluorescence Module.

Correction

An Oroboros Oxygraph-2k was used in this publication, whereas the Anton Paar/Oroboros Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the Oxygraph-2k in 2002.

Cited by

  • Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001


Labels: MiParea: Respiration, mt-Medicine  Pathology: Diabetes, Myopathy  Stress:Ischemia-reperfusion, Oxidative stress;RONS, Mitochondrial disease  Organism: Rat  Tissue;cell: Heart  Preparation: Homogenate 

Regulation: Coupling efficiency;uncoupling, mt-Membrane potential  Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

Safranin, MgG, AmR, MitoFit 2021 MgG