Difference between revisions of "Permeabilized tissue or cells"
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|abbr=P<sub>tic</sub> | |abbr=P<sub>tic</sub> | ||
|description=Permeabilized tissue (see [[permeabilized muscle fibres]]) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | |description='''Permeabilized tissue''' (see [[permeabilized muscle fibres]]) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | ||
Permeabilized cells are, therefore, not any longer [[intact cells]] or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. | '''Permeabilized cells''' are, therefore, not any longer [[intact cells]] or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. | ||
|info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized cells]] | |info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized cells]] | ||
|type=Method | |type=Method | ||
Revision as of 11:32, 31 May 2012
- high-resolution terminology - matching measurements at high-resolution
Permeabilized tissue or cells
Description
Permeabilized tissue (see permeabilized muscle fibres) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically, for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Permeabilized cells are, therefore, not any longer intact cells or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells.
Abbreviation: Ptic
Reference: MiPNet11.05; O2k-Publications: Permeabilized cells
MitoPedia methods:
Respirometry
Labels:
Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
