Difference between revisions of "Permeabilized tissue or cells"
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|abbr=pti, pce | |abbr=pti, pce | ||
|description='''Permeabilized tissue''' (see [[permeabilized muscle fibres]]) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | |description='''Permeabilized tissue''' (pti, see also [[permeabilized muscle fibres]], pfi) or cells (pce) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | ||
'''Permeabilized cells''' (pce) are, therefore, not any longer [[intact cells]] | '''Permeabilized cells''' (pce) are, therefore, not any longer viable or [[intact cells]] (ce), since the intactness of cells implies the intactness of the plasma membrane. Any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. | ||
|info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized cells]] | |info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized cells]] | ||
}}{{MitoPedia topics | }} | ||
{{MitoPedia topics | |||
|mitopedia topic=Sample preparation | |mitopedia topic=Sample preparation | ||
}} | }} | ||
Revision as of 05:13, 17 August 2016
- high-resolution terminology - matching measurements at high-resolution
Permeabilized tissue or cells
Description
Permeabilized tissue (pti, see also permeabilized muscle fibres, pfi) or cells (pce) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically, for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Permeabilized cells (pce) are, therefore, not any longer viable or intact cells (ce), since the intactness of cells implies the intactness of the plasma membrane. Any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells.
Abbreviation: pti, pce
Reference: MiPNet11.05; O2k-Publications: Permeabilized cells
MitoPedia topics:
Sample preparation
