Palty 2010 Proc Natl Acad Sci U S A: Difference between revisions
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{{Publication | {{Publication | ||
|title=Palty R, Silverman WF, Hershfinkel M, Caporale T, Sensi SL, Parnis J, Nolte C, Fishman D, Shoshan-Barmatz V, Herrmann S, Khananshvili D, Sekler Iย (2010) NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc Natl Acad Sci U S A 107:436-41. | |title=Palty R, Silverman WF, Hershfinkel M, Caporale T, Sensi SL, Parnis J, Nolte C, Fishman D, Shoshan-Barmatz V, Herrmann S, Khananshvili D, Sekler Iย (2010) NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc Natl Acad Sci U S A 107:436-41. | ||
|info=[https://pubmed.ncbi.nlm.nih.gov/20018762/ PMID: 20018762] | |info=[https://pubmed.ncbi.nlm.nih.gov/20018762/ PMID: 20018762 Open Access] | ||
|authors=Palty Raz, Silverman William F, Hershfinkel Michal, Caporale Teresa, Sensi Stefano L, Parnis Julia, Nolte Christiane, Fishman Daniel, Shoshan-Barmatz Varda, Herrmann Sharon, Khananshvili Daniel, Sekler Israel | |authors=Palty Raz, Silverman William F, Hershfinkel Michal, Caporale Teresa, Sensi Stefano L, Parnis Julia, Nolte Christiane, Fishman Daniel, Shoshan-Barmatz Varda, Herrmann Sharon, Khananshvili Daniel, Sekler Israel | ||
|year=2010 | |year=2010 |
Latest revision as of 10:53, 16 February 2022
Palty R, Silverman WF, Hershfinkel M, Caporale T, Sensi SL, Parnis J, Nolte C, Fishman D, Shoshan-Barmatz V, Herrmann S, Khananshvili D, Sekler I (2010) NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc Natl Acad Sci U S A 107:436-41. |
Palty Raz, Silverman William F, Hershfinkel Michal, Caporale Teresa, Sensi Stefano L, Parnis Julia, Nolte Christiane, Fishman Daniel, Shoshan-Barmatz Varda, Herrmann Sharon, Khananshvili Daniel, Sekler Israel (2010) Proc Natl Acad Sci U S A
Abstract: Mitochondrial Ca(2+) efflux is linked to numerous cellular activities and pathophysiological processes. Although it is established that an Na(+)-dependent mechanism mediates mitochondrial Ca(2+) efflux, the molecular identity of this transporter has remained elusive. Here we show that the Na(+)/Ca(2+) exchanger NCLX is enriched in mitochondria, where it is localized to the cristae. Employing Ca(2+) and Na(+) fluorescent imaging, we demonstrate that mitochondrial Na(+)-dependent Ca(2+) efflux is enhanced upon overexpression of NCLX, is reduced by silencing of NCLX expression by siRNA, and is fully rescued by the concomitant expression of heterologous NCLX. NCLX-mediated mitochondrial Ca(2+) transport was inhibited, moreover, by CGP-37157 and exhibited Li(+) dependence, both hallmarks of mitochondrial Na(+)-dependent Ca(2+) efflux. Finally, NCLX-mediated mitochondrial Ca(2+) exchange is blocked in cells expressing a catalytically inactive NCLX mutant. Taken together, our results converge to the conclusion that NCLX is the long-sought mitochondrial Na(+)/Ca(2+) exchanger.
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Labels: MiParea: Genetic knockout;overexpression
Regulation: Calcium, Ion;substrate transport
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