- high-resolution terminology - matching measurements at high-resolution
Ouabain
Description
Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. At low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
Abbreviation: Oua
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; FW = 728.77
Caution: Light sensitive and toxic!.
Preparation of 10 mM stock solution (dissolved in H2O):
- 1) weigh 7.3 mg of Ouabain
- 2) dissolve in 1 ml H2O
- 3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store frozen in glass vials (recommendation) at -20 °C
