Difference between revisions of "Ouabain"
From Bioblast
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|description='''Ouabain''' (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase. | |description='''Ouabain''' (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase. | ||
|info=[[MiPNet03.02]] | |info=[[MiPNet03.02]] | ||
}} {{MitoPedia topics | }} | ||
{{MitoPedia methods}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77 | '''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77. | ||
<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | <span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | ||
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'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) | ::1) Weigh 7.3 mg ouabain. | ||
::2) | ::2) Dissolve in 1 ml H<sub>2</sub>O. | ||
::3) | ::3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution. | ||
::4) | ::4) Store in glass vials (recommendation) at -20 °C. | ||
Revision as of 15:55, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Ouabain
Description
Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
Abbreviation: Oua
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; FW = 728.77.
Caution: Light sensitive and toxic!
Preparation of 10 mM stock solution (dissolved in H2O):
- 1) Weigh 7.3 mg ouabain.
- 2) Dissolve in 1 ml H2O.
- 3) Divide into 0.2 ml portions or store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the storage solution.
- 4) Store in glass vials (recommendation) at -20 °C.
