Difference between revisions of "Ouabain"

Latest revision as of 13:05, 12 February 2021

high-resolution terminology - matching measurements at high-resolution

Ouabain

Description

Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.

Abbreviation: Oua

Reference: MiPNet03.02

Application in HRR

Oua: Ouabain octahydrate (≥95% (HPLC), powder; C₂₉H₄₄O₁₂_*8H₂0); Sigma O 3125, store at R.T.; M = 728.77 g·mol^-1.

Caution: Light sensitive and toxic!

Preparation of 10 mM stock solution (dissolved in H₂O):

Weigh 7.3 mg ouabain.
Dissolve in 1 mL H₂O.
Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
Store in glass vials (recommendation) at -20 °C.

MitoPedia topics: Inhibitor

@@ Line 3: / Line 3: @@
 |description='''Ouabain''' (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
 |info=[[MiPNet03.02]]
-}} {{MitoPedia topics
-|mitopedia topic=Inhibitor
 }}
+__TOC__
 == Application in [[HRR]] ==
-'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77
+:::'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; M = 728.77 g·mol<sup>-1</sup>.
-<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>
+::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>
+::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
-'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::::# Weigh 7.3 mg ouabain.
+::::# Dissolve in 1 mL H<sub>2</sub>O.
-::1) weigh 7.3 mg of Ouabain
+::::# Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
-::2) dissolve in 1 ml H<sub>2</sub>O
+::::# Store in glass vials (recommendation) at -20 °C.
-::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock  solution
+{{MitoPedia methods}}
-::4) store frozen in glass vials (recommendation) at -20 °C
+{{MitoPedia topics
+|mitopedia topic=Inhibitor
+}}