Difference between revisions of "Ouabain"
From Bioblast
(replaced FW by M and added units g*mol-1) |
|||
| (8 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=Oua | |abbr=Oua | ||
|description=Ouabain (G-strophantin octahydrate | |description='''Ouabain''' (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase. | ||
|info=[[MiPNet03.02]] | |info=[[MiPNet03.02]] | ||
}} {{MitoPedia topics | }} | ||
__TOC__ | |||
== Application in [[HRR]] == | |||
:::'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; M = 728.77 g·mol<sup>-1</sup>. | |||
::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | |||
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# Weigh 7.3 mg ouabain. | |||
::::# Dissolve in 1 mL H<sub>2</sub>O. | |||
::::# Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution. | |||
::::# Store in glass vials (recommendation) at -20 °C. | |||
{{MitoPedia methods}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Latest revision as of 13:05, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Ouabain
Description
Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
Abbreviation: Oua
Reference: MiPNet03.02
Application in HRR
- Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; M = 728.77 g·mol-1.
- Caution: Light sensitive and toxic!
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 7.3 mg ouabain.
- Dissolve in 1 mL H2O.
- Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
- Store in glass vials (recommendation) at -20 °C.
MitoPedia topics:
Inhibitor
