Oroboros FAQ: Difference between revisions
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== O2k FAQ == | |||
== APPLICATIONS == | === APPLICATIONS AND SAMPLES === | ||
< | <div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Which are the most common applications of the [[Oxygraph-2k|OROBOROS Oxygraph-2k]] (O2k)?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | ''The most common applications for the O2k are the following'': | ||
* Diagnosis of acquired and genetic mitochondrial diseases | |||
* Diagnosis of myopathies and neuromuscular pathologies | |||
* Studies on cell function and cell death - apoptosis and necrosis | |||
* Measurement of oxidative stress | |||
* Studies on ischemia-reperfusion injury | |||
* Studies on aging and senescence | |||
* Pharmacological tests | |||
For more applications, browse through the [[O2k-Publications]]. | |||
<div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">What is high-resolution respirometry and what is so special about it?</div> | |||
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. | |||
O2k [[High-Resolution_Respirometry|high-resolution respirometry (HRR)]] is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features [[DatLab|(DatLab)]] to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation. | |||
<div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Is the O2k easy to use in standard applications of mitochondrial respiration?</div> | |||
The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: [[DatLab]] | The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: [[DatLab]] | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?</div> | ||
The O2k can be routinely applied for cultured cells, intact cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: [[Respirometry]] | The O2k can be routinely applied for cultured cells, [[Intact cells|intact cells]] and tissue biopsies, [[Permeabilized tissue or cells|permeabilized]] cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, [[Isolated mitochondria|isolated mitochondria]]. See: [[Respirometry]] and [[O2k-Publications]] | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?</div> | ||
The O2k is a | Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personell- and time-efficient. | ||
<div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Can the O2k be applied for measuring human biopsies?</div> | |||
When [[Permeabilized tissue or cells|permeabilized]] biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols. | |||
<div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Can the O2k be applied to measure tissue oxygen concentration?</div> | |||
The O2k is a [[O2k-Chamber|'chamber']] approach, for application with tissue biopsies, cultured cells or [[Isolated mitochondria|isolated mitochondria]]. Whereas HRR cannot be applied for measurement of tissue O<sup>2</sup> concentration, it is the method of choice for analysis of tissue O<sup>2</sup> consumption and functional diagnosis of mitochondrial injuries. | |||
<div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">Can the O2k be applied for the measurement of oxygen consumption in [[Intact cells|adherent cells]]?</div> | |||
O2k | Attached cells may be grown on disks before being introduced into the [[O2k-Chamber|cylindrical chamber]] of the O2k. Alternatively, cells attached to beads can be used. With this method, however, gentle stirring, with the use of specific small [[Stirrer-Bar\white PVDF\15x6 mm|stirring bars]], is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, 200,000 cells per chamber are required. | ||
<div style="border-bottom:1px solid #abd5f5; background:#CEF2E0; font-size:110%; font-weight:bold;">How many parameters can be measured simultaneously?</div> | |||
The O2k is designed to measure respiration plus one additional parameter at a time. | |||
=== SOFTWARE COMPATIBILITY === | |||
<div style="border-bottom:1px solid #abd5f5; background:#CEE0F2; font-size:110%; font-weight:bold;">Does [[DatLab]] software run on MACs?</div> | |||
No. As of now [[DatLab]] software is designed for Windows operating systems only. | |||
=== HOW AND WHERE TO BUY === | |||
<div style="border-bottom:1px solid #abd5f5; background:#E0CEF2; font-size:110%; font-weight:bold;">Do you have any distributors in my country?</div> | |||
(exclusive distributors in XXX, direct sales approach, if necessary cooperate with a distributor you choose) | |||
<div style="border-bottom:1px solid #abd5f5; background:#E0CEF2; font-size:110%; font-weight:bold;">How much is the O2k?</div> | |||
<div style="border-bottom:1px solid #abd5f5; background:#E0CEF2; font-size:110%; font-weight:bold;">Who do I contact to buy an O2k</div> | |||
=== O2K IN COMPARISSON WITH OTHER DEVICES === | |||
= | <div style="border-bottom:1px solid #abd5f5; background:#F2CEE0; font-size:110%; font-weight:bold;">Why buy an O2k and not a different oxygraph?</div> | ||
[http://wiki.oroboros.at/index.php/File:MiPNet14.10_O2k-Top10.pdf Top 10 reasons for the OROBOROS Oxygraph-2k] | |||
=== DELIVERY CONTENT, WARRANTY AND SUPPORT === | |||
= | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">How long from order till delivery?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Does the purchase include installation and setup and training?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Do you have an after-sales service contract?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Does the [[O2k-Core|O2k-Core]] include a PC or do we have to purchase it separately?</div> | ||
Verlinkung zu PC requirements und dann auch electrical requirements | Verlinkung zu PC requirements und dann auch electrical requirements | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Is it possible to extend the warranty period?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Are taxes and duty fees included in the price?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Do you offer the option to lease or rent your instruments?</div> | ||
<div style="border-bottom:1px solid #abd5f5; background:# | <div style="border-bottom:1px solid #abd5f5; background:#F2E0CE; font-size:110%; font-weight:bold;">Are you registered with System for Award Management (SAM)?</div> | ||
Revision as of 15:33, 21 December 2015
O2k FAQ
APPLICATIONS AND SAMPLES
Which are the most common applications of the OROBOROS Oxygraph-2k (O2k)?
The most common applications for the O2k are the following:
- Diagnosis of acquired and genetic mitochondrial diseases
- Diagnosis of myopathies and neuromuscular pathologies
- Studies on cell function and cell death - apoptosis and necrosis
- Measurement of oxidative stress
- Studies on ischemia-reperfusion injury
- Studies on aging and senescence
- Pharmacological tests
For more applications, browse through the O2k-Publications.
What is high-resolution respirometry and what is so special about it?
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry.
O2k high-resolution respirometry (HRR) is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation.
Is the O2k easy to use in standard applications of mitochondrial respiration?
The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: DatLab
Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?
The O2k can be routinely applied for cultured cells, intact cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: Respirometry and O2k-Publications
Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?
Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personell- and time-efficient.
Can the O2k be applied for measuring human biopsies?
When permeabilized biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols.
Can the O2k be applied to measure tissue oxygen concentration?
The O2k is a 'chamber' approach, for application with tissue biopsies, cultured cells or isolated mitochondria. Whereas HRR cannot be applied for measurement of tissue O2 concentration, it is the method of choice for analysis of tissue O2 consumption and functional diagnosis of mitochondrial injuries.
Can the O2k be applied for the measurement of oxygen consumption in adherent cells?
Attached cells may be grown on disks before being introduced into the cylindrical chamber of the O2k. Alternatively, cells attached to beads can be used. With this method, however, gentle stirring, with the use of specific small stirring bars, is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, 200,000 cells per chamber are required.
How many parameters can be measured simultaneously?
The O2k is designed to measure respiration plus one additional parameter at a time.
SOFTWARE COMPATIBILITY
Does DatLab software run on MACs?
No. As of now DatLab software is designed for Windows operating systems only.
HOW AND WHERE TO BUY
Do you have any distributors in my country?
(exclusive distributors in XXX, direct sales approach, if necessary cooperate with a distributor you choose)
How much is the O2k?
Who do I contact to buy an O2k
O2K IN COMPARISSON WITH OTHER DEVICES
Why buy an O2k and not a different oxygraph?
Top 10 reasons for the OROBOROS Oxygraph-2k
DELIVERY CONTENT, WARRANTY AND SUPPORT
How long from order till delivery?
Does the purchase include installation and setup and training?
Do you have an after-sales service contract?
Does the O2k-Core include a PC or do we have to purchase it separately?
Verlinkung zu PC requirements und dann auch electrical requirements
Is it possible to extend the warranty period?
Are taxes and duty fees included in the price?
Do you offer the option to lease or rent your instruments?
Are you registered with System for Award Management (SAM)?
