OctPM pathway control state: Difference between revisions
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'''MitoPathway control:''' FAO<small>&</small>CI | '''MitoPathway control:''' FAO<small>&</small>CI | ||
'''SUIT protocol:''' [[SUIT RP1 | '''SUIT protocol:''' [[SUIT_FNSGp(PGM)01]] - SUIT RP1, [[SUIT_FNSGp(PGM)02]] - SUIT RP1 | ||
This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[Malate alone]], and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state. | This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[Malate alone]], and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state. | ||
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== OctPM(P) == | == OctPM(P) == | ||
# [[ | # [[SUIT_FNSGp(PGM)02]]_'''4P''': 1D 2Oct 3Mtit&Mc (&McNADH) '''<u>4P</u>''' 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd''' | ||
== OctPM(E) == | == OctPM(E) == | ||
# [[ | # [[SUIT_FNSGp(PGM)01]]_'''4Oct''': 1PM 2D&Dc (&DcNADH) 3U '''<u>4Oct</u>''' 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd | ||
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Revision as of 07:07, 9 September 2016
- high-resolution terminology - matching measurements at high-resolution
OctPM pathway control state
Description
OctPM: Octanoylcarnitine & Pyruvate & Malate.
MitoPathway control: FAO&CI
SUIT protocol: SUIT_FNSGp(PGM)01 - SUIT RP1, SUIT_FNSGp(PGM)02 - SUIT RP1
This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare Malate alone, and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state.
Abbreviation: PMOct
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
Respiratory state,
SUIT state
OctPM(L)
OctPM(P)
- SUIT_FNSGp(PGM)02_4P: 1D 2Oct 3Mtit&Mc (&McNADH) 4P 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd
OctPM(E)
- SUIT_FNSGp(PGM)01_4Oct: 1PM 2D&Dc (&DcNADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd
