Difference between revisions of "OXPHOS capacity"
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|abbr=''P'' | |abbr=''P'' | ||
|description='''OXPHOS capacity''' (''P''; compare [[State 3]]) is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]], inorganic phosphate, oxygen, and defined reduced substrates. Since OXPHOS is partially coupled, intrinsic [[uncoupling]] and [[dyscoupling]] contribute to the control of flux in the OXPHOS state (state ''P''). OXPHOS capacity is expressed (i) per mt-marker (O<sub>2</sub> [[flux]] per mt-protein, [[CS]], etc); if ETS capacity, ''E'', is used as a functional mitochondrial marker, then OXPHOS capacity is expressed as the ''[[P/E]]'' ratio ([[flux control ratio]]). (ii) OXPHOS capacity is expressed per tissue or cell mass, integrating mt-quantity (density) and mt-quality (O<sub>2</sub> flux). (iii) OXPHOS capacity is expressed per cell (O<sub>2</sub> [[flow]]), which then is a function of mt-density, mt-quality, and cell size. | |description='''OXPHOS capacity''' (''P''; compare [[State 3]]) is the respiratory capacity of mitochondria in the ADP-activated state of [[oxidative phosphorylation]], at saturating concentrations of [[ADP]], inorganic phosphate, oxygen, and defined reduced substrates. Since OXPHOS is partially coupled, intrinsic [[uncoupling]] and [[dyscoupling]] contribute to the control of flux in the OXPHOS state (state ''P''). OXPHOS capacity is expressed (i) per mt-marker (O<sub>2</sub> [[flux]] per mt-protein, [[Citrate synthase|CS]], etc); if ETS capacity, ''E'', is used as a functional mitochondrial marker, then OXPHOS capacity is expressed as the ''[[P/E]]'' ratio ([[flux control ratio]]). (ii) OXPHOS capacity is expressed per tissue or cell mass, integrating mt-quantity (density) and mt-quality (O<sub>2</sub> flux). (iii) OXPHOS capacity is expressed per cell (O<sub>2</sub> [[flow]]), which then is a function of mt-density, mt-quality, and cell size. | ||
|info=[http://www.oroboros.at/?RespiratoryStates MiPNet12.15], [[Gnaiger 2009 Int J Biochem Cell Biol]] | |info=[http://www.oroboros.at/?RespiratoryStates MiPNet12.15], [[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
|type=Respiration | |type=Respiration | ||
Revision as of 10:39, 25 June 2012
- high-resolution terminology - matching measurements at high-resolution
OXPHOS capacity
Description
OXPHOS capacity (P; compare State 3) is the respiratory capacity of mitochondria in the ADP-activated state of oxidative phosphorylation, at saturating concentrations of ADP, inorganic phosphate, oxygen, and defined reduced substrates. Since OXPHOS is partially coupled, intrinsic uncoupling and dyscoupling contribute to the control of flux in the OXPHOS state (state P). OXPHOS capacity is expressed (i) per mt-marker (O2 flux per mt-protein, CS, etc); if ETS capacity, E, is used as a functional mitochondrial marker, then OXPHOS capacity is expressed as the P/E ratio (flux control ratio). (ii) OXPHOS capacity is expressed per tissue or cell mass, integrating mt-quantity (density) and mt-quality (O2 flux). (iii) OXPHOS capacity is expressed per cell (O2 flow), which then is a function of mt-density, mt-quality, and cell size.
Abbreviation: P
Reference: MiPNet12.15, Gnaiger 2009 Int J Biochem Cell Biol
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
