O2k signals and output

Signal of the O2k-Core and add-on modules

Three channel types are available in the O2k-MultiSensor system:

1. O₂ channel with electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The O2k-Core supports not only the O₂ channel, but also the channel types 2 and 3 for the add-on modules.
2. Amp channel: The amperometric channel of the O2k, has electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp-Channel is used with the O2k-Fluo LED2-Module and the O2k-NO Amp-Module.
3. pX channel with electric potential (volt [V]) as the primary and raw signal (BNC plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref).

Additional channel types are available in the NextGen-O2k:

4. Spectrophotometric channel.

5. Dual-fluo channel.

Output

The 'output' of a channel is the finally important infomation, which may be summarized in a data base. Three types of output can be derived from the signal of the O2k channels:

A: Concentration (e.g. O₂; Ca²⁺).

B: Flow, flux, rate (e.g. O₂ consumption; H₂O₂ production).

C: Force, potential (e.g. TMRM and Safranin for mt-membrane potential; redox potential from cytochrome or NADH redox states).

1. O₂ channel - O2k-Core: Output type A and B.

2a. Amp channel - O2k-Fluo LED2-Module: The focus of the output depends on the specific fluorophore. Output type A, B, or C, or any combination may relate to the primary level of interest.

2b. Amp channel - O2k-NO Amp-Module: Output type A and B.

3a. pX channel - O2k-TPP+ ISE-Module: Output type C.

3b. pX channel - O2k-pH ISE-Module: Output type A and B.

4. Spectrophotometric channel - NextGen-O2k: Output type D (three plots for redox states of cytochromes aa₃, b, and c.

5. Dual-fluo channel - NextGen-O2k: Plots of two selected wavelengths can be selected for simultaneous recording. Output type E1, E2, .. depends on the fluorophore.