Difference between revisions of "O2k signals and output"
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== Signal of the O2k-Core and add-on modules == | == Signal of the O2k-Core and add-on modules == | ||
Three channel types are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]: | Three electronic channel types are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold, for O2k-Chambers A and B (left and right): | ||
# O<sub>2</sub> channel with electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The O2k-Core supports not only the O<sub>2</sub> channel, but also the channel types 2 and 3 for the add-on modules. | # O<sub>2</sub> channel with electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The O2k-Core supports not only the O<sub>2</sub> channel (O2 plug), but also the channel types 2 and 3 for the add-on modules. | ||
# Amp channel: The amperometric channel of the O2k, has electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp-Channel is used with the [[O2k-Fluo LED2-Module]] and the [[O2k-NO Amp-Module]]. | # Amp channel: The amperometric channel of the O2k, has electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp-Channel is used with the [[O2k-Fluo LED2-Module]] and the [[O2k-NO Amp-Module]]. | ||
# pX channel with electric potential (volt [V]) as the primary and raw signal (BNC plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref). | # pX channel with electric potential (volt [V]) as the primary and raw signal (BNC plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref). | ||
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: 1. O<sub>2</sub> channel - [[O2k-Core]]: Output type A and B. | : 1. O<sub>2</sub> channel - [[O2k-Core]]: Output type '''A''' and '''B'''. | ||
: 2a. Amp channel - [[O2k-Fluo LED2-Module]]: The focus of the output depends on the specific fluorophore. Output type A, B, or C, or any combination may relate to the primary level of interest. | : 2a. Amp channel - [[O2k-Fluo LED2-Module]]: The focus of the output depends on the specific fluorophore. Output type '''A''', '''B''', or '''C''', or any combination may relate to the primary level of interest. | ||
: 2b. Amp channel - [[O2k-NO Amp-Module]]: Output type A and B. | : 2b. Amp channel - [[O2k-NO Amp-Module]]: Output type '''A''' and '''B'''. | ||
: 3a. pX channel - [[O2k-TPP+ ISE-Module]]: Output type C. | : 3a. pX channel - [[O2k-TPP+ ISE-Module]]: Output type '''C'''. | ||
: 3b. pX channel - [[O2k-pH ISE-Module]]: Output type A and B. | : 3b. pX channel - [[O2k-pH ISE-Module]]: Output type '''A''' and '''B'''. | ||
: 4. Spectrophotometric channel - [[NextGen-O2k]]: Output type D (three plots for redox states of cytochromes ''aa''<sub>3</sub>, ''b'', and ''c''. | : 4. Spectrophotometric channel - [[NextGen-O2k]]: Output type '''D''' (three plots for redox states of cytochromes ''aa''<sub>3</sub>, ''b'', and ''c''. | ||
: 5. Dual-fluo channel - [[NextGen-O2k]]: Plots of two selected wavelengths can be selected for simultaneous recording. Output type E1, E2, .. depends on the fluorophore. | : 5. Dual-fluo channel - [[NextGen-O2k]]: Plots of two selected wavelengths can be selected for simultaneous recording. Output type '''E1''', '''E2''', .. depends on the fluorophore. | ||
Revision as of 15:44, 28 July 2015
Signal of the O2k-Core and add-on modules
Three electronic channel types are available in the O2k-MultiSensor system. All channels are available twofold, for O2k-Chambers A and B (left and right):
- O2 channel with electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The O2k-Core supports not only the O2 channel (O2 plug), but also the channel types 2 and 3 for the add-on modules.
- Amp channel: The amperometric channel of the O2k, has electric current (ampere [Amp]) as the primary signal, which is converted to a voltage (volt [V]) as the raw signal. The Amp-Channel is used with the O2k-Fluo LED2-Module and the O2k-NO Amp-Module.
- pX channel with electric potential (volt [V]) as the primary and raw signal (BNC plug for the ion sensitive electrode, ISE; and separate plug for the pX reference electrode, Ref).
Additional channel types are available in the NextGen-O2k:
- 4. Spectrophotometric channel.
- 5. Dual-fluo channel.
Output
The 'output' of a channel is the finally important infomation, which may be summarized in a data base. Three types of output can be derived from the signal of the O2k channels:
- A: Concentration (e.g. O2; Ca2+).
- B: Flow, flux, rate (e.g. O2 consumption; H2O2 production).
- C: Force, potential (e.g. TMRM and Safranin for mt-membrane potential; redox potential from cytochrome or NADH redox states).
- 1. O2 channel - O2k-Core: Output type A and B.
- 2a. Amp channel - O2k-Fluo LED2-Module: The focus of the output depends on the specific fluorophore. Output type A, B, or C, or any combination may relate to the primary level of interest.
- 2b. Amp channel - O2k-NO Amp-Module: Output type A and B.
- 3a. pX channel - O2k-TPP+ ISE-Module: Output type C.
- 3b. pX channel - O2k-pH ISE-Module: Output type A and B.
- 4. Spectrophotometric channel - NextGen-O2k: Output type D (three plots for redox states of cytochromes aa3, b, and c.
- 5. Dual-fluo channel - NextGen-O2k: Plots of two selected wavelengths can be selected for simultaneous recording. Output type E1, E2, .. depends on the fluorophore.
