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{{Technical support}} __TOC__ {{MitoPedia without banner |abbr= |description=[[Image:O2k-Titration Set.JPG|right|180px]] The '''O2k-Titration Set''' consists of Hamilton microsyringes (6 x 10 mm<sup>3</sup> and 3 spare plungers, 6 x 25 mm<sup>3</sup>, 1 x 50 mm<sup>3</sup>, 1 x 100 mm<sup>3</sup>, 1 x 500 mm<sup>3</sup>; fixed needles with rounded tips), provided in the [[Syringe Storage Box]] with [[Syringe Labels]], a set of two [[Syringe Racks]] with [[Syringe Collars]], and a set of two [[Tube Racks]]. |info= [https://www.oroboros.at/index.php/product/o2k-titration-set/ '''Product details and purchasing information'''] }} == Additional resources == :::: Β»[[MiPNet09.12 O2k-Titrations]] </br> :::: Β»[[MiPNet19.14 SOP Hamilton microsyringes]] == Note: Titration of chemicals in respirometry == :::: Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water. :::: After a titration, it is common to observe liquid accumulated on top of the stopper receptacle. When this happens, we recommend removing this excess of liquid as it can lead to O<sub>2</sub> backdiffusion into the chamber. For this, we recommend using the [[ISS]], placing the tip close to the capillary, but not directly on top of it. === Further information === :::* Safety information: fixed needles are used with rounded tips, not usable for injection into tissues. :::* Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions. :::* Visit also: [http://www.hamiltoncompany.com/products/syringes-and-needles Hamilton]. :::* Eliminate rough edges with 3Mβ’ Wetordryβ’ Paper Sheet P1000 by gentle rubbing. :::* Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H<sub>2</sub>O and try to press the plunger gently down.
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