Difference between revisions of "Mitochondrial preparations"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=mtprep | |abbr=mtprep | ||
|description='''Mitochondrial preparations''' (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the | |description='''Mitochondrial preparations''' (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent the mt-structure is maintained. According to this definition, submitochondrial particles (smtp) are not a mtprep. | ||
}} | }} | ||
Β Communicated by [[Gnaiger E]] 2011-11-15 (last edit 2022-12-11). | |||
Β Communicated by [[Gnaiger E]] 2011-11-15 | |||
== MitoPedia: mt-preparations == | == MitoPedia: mt-preparations == | ||
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{{Labeling | {{Labeling | ||
|preparations=Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate | |preparations=Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate | ||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Sample preparation | |||
}} | }} |
Revision as of 13:51, 11 December 2022
Description
Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent the mt-structure is maintained. According to this definition, submitochondrial particles (smtp) are not a mtprep.
Abbreviation: mtprep
Communicated by Gnaiger E 2011-11-15 (last edit 2022-12-11).
MitoPedia: mt-preparations
- Isolated mitochondria, imt
- Tissue homogenate, thom
- Permeabilized tissue, pti
- Permeabilized muscle fibers, pfi
- Permeabilized cells, pce
- Β» Compare: Submitochondrial particles, smtp
- O2k-Protocols for mt-preparations: O2k-mitochondrial preparations
References
Bioblast link | Reference | Year |
---|---|---|
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
Labels:
Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate
MitoPedia topics: Sample preparation