MitoPediaAlert2020 Archive: Difference between revisions
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== | == Jul 2020 == | ||
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* '''Jul-02 Th''' | |||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br> | |||
- loss of oxidative capacity during the time course of the respirometric assay,<br> | |||
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> | |||
- over titration of an uncoupler, which inhibits ET capacity,<br> | |||
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity. | |||
Information from: Gnaiger Erich ''et al'' β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Β»[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]Β« | |||
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* '''Jul-09 Th''' | |||
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:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br> | |||
- Β»'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''Β« | |||
|} | |||
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== Previous MitoPedia Alert 2020 == | |||
[[File:Expand.png|right|35px |Click to expand or collaps]] | |||
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"> | |||
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span> | |||
<div class="mw-collapsible-content"> | |||
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== Jun 2020 == | |||
* '''Jun-04 Th''' | |||
:{| | |||
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | |||
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br> | |||
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallelΒ to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br> | |||
-Β»'''[[SUIT-008]]'''Β« and Β»'''[[SUIT-014]]'''Β« | |||
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* '''Jun-08 Mo''' Β | * '''Jun-08 Mo''' Β | ||
:{| | :{| | ||
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- Β» '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''Β« | - Β» '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''Β« | ||
|} | |} | ||
=== May 2020 === | === May 2020 === |
Revision as of 08:15, 6 July 2020
- Currently Answered Questions from the Β»Oroboros Ecosystem AgendaΒ«
Jul 2020
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Β»Bioblast linkΒ«
- Jul-09 Th
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?