MitoKit-CII/Malonate-nv: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 7: | Line 7: | ||
|mitopedia topic=Medium | |mitopedia topic=Medium | ||
}} | }} | ||
* | ย | ||
== Application in HRR == | |||
:::: '''Mnanv: NV161''' 10 mg, 7.7 ยตL, 5.24 M, MW=248.1 g.mol<sup>-1</sup> | |||
:::: '''Preparation of 100 mM stock solution:''' | |||
::::# Add 395.78 ยตL DMSO in 7.7 ยตL NV161. | |||
::::# Divide into 40 ยตL portions. | |||
::::# Store at -20 ยฐC. | |||
ย | |||
:::: '''Preparation of 10 mM stock solution (from the 100 mM stock):''' | |||
::::# Dilute 40 ยตL 100 mM stock solution with 360 ยตL DMSO. | |||
::::# Divide into 100 ยตL portions.ย | |||
::::# Store at -20 ยฐC. | |||
ย | |||
:::ยป '''O2k manual titrations''' | |||
::::* Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber). | |||
::::* Final concentration: 5 ยตM steps | |||
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type. |
Revision as of 15:10, 11 April 2019
Description
NV161/MitoKit-CII (diacetoxymethyl malonate) is a plasma membrane-permeable (p) prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space.
Abbreviation: Mnanv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Mnanv: NV161 10 mg, 7.7 ยตL, 5.24 M, MW=248.1 g.mol-1
- Preparation of 100 mM stock solution:
- Add 395.78 ยตL DMSO in 7.7 ยตL NV161.
- Divide into 40 ยตL portions.
- Store at -20 ยฐC.
- Preparation of 10 mM stock solution (from the 100 mM stock):
- Dilute 40 ยตL 100 mM stock solution with 360 ยตL DMSO.
- Divide into 100 ยตL portions.
- Store at -20 ยฐC.
- Preparation of 10 mM stock solution (from the 100 mM stock):
- ยป O2k manual titrations
- Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 5 ยตM steps
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- ยป O2k manual titrations