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MitoFit K-Regio project report

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Bioblast 2022



MitoFit K-Regio project report

Mitochondrial fitness - a new O2k perspective


» MitoFit - mitochondria fit and well

MitoFit track record

  1. 2016-01-24 MitoPedia: Sample preparations
  2. 2016-01-24 MitoPedia: SUIT (update: 2016-05-13)
» O2k-Publications: Cryopreservation
» Microwell respirometry


MitoFit publications

   
MiPNet28.08 Oroboros O2k Series J manual2023-10-19
O2k-Manual
Oroboros O2k Series J manual
MiPNet28.07 NextGen-O2k Series XB manual2023-10-19
O2k-Manual
NextGen-O2k Series XB manual
MiPNet26.13 NextGen-O2k manual2022-07-25
O2k-Manual
NextGen-O2k manual
MiPNet26.12 NextGen-O2k: NADH-Module2022-05-17
O2k-Manual
NextGen-O2k: NADH-Module manual
MiPNet24.12 NextGen-O2k: Q-Module2021-10-29
O2k-Manual
NextGen-O2k: Q-Module manual
MiPNet26.11 NextGen-O2k: PB-Module2021-10-29
O2k-Manual
NextGen-O2k: PB-Module manual
MiPNet24.10 H2O2 flux analysis2021-10-22
O2k-Protocols
Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
MiPNet26.10 MgG data analysis2021-09-16
O2k-Protocols
Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4
BEC 2020.1 doi10.26124bec2020-0001.v12020Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
MitoEAGLE blood cells 12020Åsander Frostner Eleonor*, Aburel Oana M*, Avram Vlad F*, Calabria Elisa, Castelo Rueda Maria Paulina*, Chamkha Imen*, ČiĆŸmĂĄrovĂĄ Beata, Danila Maria-Daniela*, Doerrier Carolina*, Eckert Gunter P*, Ehinger Johannes K, Elmer Eskil*, Garcia-Souza Luiz F*, Gnaiger Erich*, Hoppel Florian*, Karabatsiakis Alexander*, Keppner Gloria, Kidere Dita*, Krako Jakovljević Nina, Labieniec-Watala Magdalena*, Lelcu Theia*, Micankova Petra, Michalak Slawomir*, Molina Anthony JA*, Pavlovic Kasja, Pichler Irene*, Piel Sarah, Rousar Tomas, Rybacka-Mossakowska Joanna, Schartner Melanie, Siewiera Karolina*, Silaidos Carmina*, Sjövall Fredrik*, Sobotka Ondrej*, Sumbalova Zuzana*, Swiniuch Daria, Vernerova Andrea*, Volani Chiara*, Vujacic-Mirski Ksenija*, Watala Cezary* (2020) Interlaboratory guide to mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets. - Updated: 2020-03-06 - *Confirmed
Gnaiger 2020 BEC MitoPathways2020Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
MitoEAGLE muscle 12020Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. - Updated: 2020-03-08
MiPNet24.11 mtMP calculation2019-08-05
O2k-Protocols
Mitochondrial membrane potential calculation
MiPNet20.14 AmplexRed H2O2-production2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
MiPNet20.13 Safranin mt-membranepotential2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
MiPNet21.06 SUIT RP2018-06-25
O2k-Protocols
SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MĂ©szĂĄros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3
Miller 2017 PLOS ONE2017Miller B, Hamilton K, Boushel R, Williamson K, Laner V, Gnaiger E, Davis M (2017) Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLOS ONE 12:e0174874.
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
Schoepf 2016 FEBS J2016Schöpf B, SchÀfer G, Weber A, Talasz H, Eder IE, Klocker H, Gnaiger E (2016) Oxidative phosphorylation and mitochondrial function differ between human prostate tissue and cultured cells. https://doi.org/10.1111/febs.13733
Harrison 2015 J Appl Physiol2015Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. https://doi.org/10.1152/japplphysiol.00146.2015
Gnaiger 2015 Scand J Med Sci Sports2015Gnaiger E, Boushel R, SĂžndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DĂ­ez-SĂĄnchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612
Boushel 2015 Scand J Med Sci Sports2015Boushel R, Gnaiger E, Larsen FJ, Helge JW, Gonzalez-Alonso J, Ara I, Munch-Andersen T, van Hall G, Sþndergaard H, Saltin B, Calbet JAL (2015) Maintained peak leg and pulmonary VO2 despite substantial reduction in muscle mitochondrial capacity. Scand J Med Sci Sports 25 (Suppl 4):135–43.


MitoFit events

 WhenWhere
MiPNet28.12 IOC167 Schroecken AT2024-09-30
O2k-Workshops.png
Schroecken AT, 2024 Sep 30 - Oct 05. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC167.
IOC166 Ljubljana SI2024-09-22
O2k-Workshops.png
Ljubljana SI, 2024 Sep 22 - Sep 26. Satellite symposium and workshop "Skeletal Muscle Research – from Cell to Human", IOC166.
MiPNet28.13 IOC164 Innsbruck AT2024-09-02
O2k-Workshops.png
Innsbruck AT, 2024 Sep 02-04. EBEC2024 Satellite Oroboros O2k-Workshop: Mito&Chlora High-Resolution Respirometry and PhotoBiology, IOC164.
MiPNet28.11 IOC163 Schroecken AT2024-06-17
O2k-Workshops.png
Schroecken AT, 2024 Jun 17-22. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC163.
MiPNet28.02 IOC162 Schroecken AT2023-10-02
O2k-Workshops.png
Schroecken AT, 2023 Oct 02-07. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC162.
MiPNet28.01 IOC160 Schroecken AT2023-06-19
O2k-Workshops.png
Schroecken AT, 2023 Jun 19-24. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC160.
MiPNet28.05 IOC158 Innsbruck AT2023-02-27
OROBOROS-solo.png
Innsbruck AT, 2023 Feb 27 - Mar 01. O2k-Coaching Days - Basic, IOC158.
MiPNet27.04 IOC155 Schroecken AT2022-10-03
O2k-Workshops.png
Schroecken AT, 2022 Oct 03-08. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC155.
MiPNet25.02 IOC Schroecken AT2020
O2k-Workshops.png
Schroecken AT, Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC. - Due to COVID-19 we change the strategy and provide the O2k-Workshop in a virtual form.
MiPNet24.15 IOC143 Haryana IN2019-11-15
O2k-Network
Haryana IN, 2019 Nov 15. Oroboros O2k-Workshop on high-resolution respirometry: Assessment of mitochondrial function in health and disease IOC143.
MiPNet24.02 IOC141 Schroecken AT2019-09-23
NextGenO2k logo.jpg
Schroecken AT, 2019 Sep 23-28 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC141.
MiPNet24.01 IOC139 Schroecken AT2019-06-17
O2k-Workshops.png
Schroecken AT, 2019 June 17-22 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139.
DSL Retreat 2019 Innsbruck AT2019-02-21Vill AT, 2019 Feb 21. Third retreat of the Daniel Swarovski Research Laboratory (DSL).
MitoFit Conference 2019 Innsbruck AT2019-01-29
Logo MitoFit.jpg
Innsbruck AT, 2019 Jan 29. MitoFit Conference: Mitochondrial fitness in cancer metabolism, exercise and aging.
MiPNet23.11 IOC137 Innsbruck AT2018-12-10
OROBOROS-solo.png
Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC137.
PartneringDay 2018 Innsbruck AT2018-10-11Innsbruck AT, 2018 Oct 11. Partnering Day 2018
Fokus Tag: Targeting Phospholipids 2018 Heidelberg DE2018-10-05Heidelberg DE, 2018 Oct 05. Fokus Tag: Targeting Phospholipids.
MiPNet23.08 IOC134 Schroecken AT2018-10-01
OROBOROS-solo.png
Schroecken AT, 2018 Oct 01-06 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC.
Fokus Tag: Labordiagnostik Intensiv 2018 Heidelberg DE2018-09-16Heidelberg DE, 2018 Sep 16. Fokus Tag: Labordiagnostik Intensiv.
MiPNet23.04 IOC133 Innsbruck AT2018-09-05
OROBOROS-solo.png
Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC 133.
MiPNet23.06 IOC130 Schroecken AT2018-06-18
OROBOROS-solo.png
Schroecken AT, 2018 Jun 18-23 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC130.
Long Night of Research 2018 Innsbruck AT2018-04-13
LNF.JPG
Innsbruck AT, 2018 Apr 13. Oroboros at Long Night of Research, CCB. The diagnostic bioenergetic report – a milestone on the way to mitochondrial fitness and physical well-being.
Clinical Mitochondria- and Environmental Medicine 2018 Heidelberg DE2018-03-02Heidelberg DE, 2018 Mar 02-03. Clinical Mitochondria- and Environmental Medicine.
DSL retreat Innsbruck AT2018-02-15Vill AT, 2018 Feb 15. Second retreat of the Daniel Swarovski Research Laboratory (DSL).
MitoEAGLE Innsbruck 2018-01-292018-01-29
COST Action MitoEAGLE
Innsbruck AT, 2018 Jan 29 - Feb 01. WG 4 blood cells workshop and retreat - COST Action MitoEAGLE.
MiPNet23.01 IOC126 Innsbruck AT2018-01-24
OROBOROS-solo.png
Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126.
MitoFit Workshop ATP 2017 Innsbruck AT2017-12-18
MitoFit
Innsbruck AT, 2017 Dec 18-20. MitoFit Workshop on adenosine triphosphate (ATP).
MitoFit Open Seminar 2017-10-232017-10-23
MitoFit
Innsbruck AT, 2017 Oct 23. Daniel-Swarovski-Research Laboratory Research Seminar WS 2017/2018 - MitoFit Open Seminar on Respiration, cryopreservation and viability test in human blood cells: peripheral blood mononuclear cells (PBMC) and platelets.
MiPNet22.07 IOC124 Schroecken AT2017-10-03
OROBOROS-solo.png
Schroecken AT, 2017 Oct 03-08 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC124.
MitoEAGLE Obergurgl 20172017-07-27
COST Action MitoEAGLE
Obergurgl AT, 2017 Jul 27-30. 10th MitoEAGLE Workshop WG1-4.
MiPschool Obergurgl 20172017-07-23
COST Action MitoEAGLE
MiPsociety
Obergurgl AT, 2017 Jul 23-30. 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4.
MitoFit Open Seminar 2017-07-142017-07-14
MitoFit
Innsbruck AT, 2017 Jul 14. MitoFit Open Seminar on immune cell bioenergetics.
Investigating the role of mitochondria in healthy and psychopathological aging.
MiPNet22.01 IOC122 Schroecken AT2017-06-26
OROBOROS-solo.png
Schroecken AT, 2017 Jun 26-Jul 01 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC122.
MiPNet22.06 IOC121 Cologne DE2017-06-12
Oroboros Instruments
Cologne DE, 2017 Jun 12. Oroboros O2k-Lunch Workshop on high-resolution respirometry (HRR), IOC121 at EUROMIT2017 Cologne DE.
Life Science PhD Meeting 2017 Innsbruck AT2017-04-18Innsbruck AT, 2017 Apr 18-19. Careers in Industry and PhD LifeScience Symposium.
Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE2017-03-11Heidelberg DE, 2017 Mar 11-12. Klinische Mitochondrienmedizin und Umweltmedizin.
MiPNet22.03 IOC119 Innsbruck AT2017-02-27
OROBOROS-solo.png
Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.
MiPNet21.19 IOC114 Innsbruck AT2016-12-12
OROBOROS-solo.png
Innsbruck AT, 2016 Dec 12-15. MitoFit Coaching Days - Basic IOC114.
MiPNet21.11 IOC116 Innsbruck AT2016-11-21
OROBOROS-solo.png
Innsbruck AT, 2016 Nov 21-25. Oroboros O2k-Workshop on O2k-Fluorometry - advanced, IOC116.
MiPNet21.10 IOC115 Schroecken AT2016-10-03
OROBOROS-solo.png
Schroecken AT, 2016 Oct 03-08. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC115.
MiPNet21.18 IOC117 Kuala Lumpur MY2016-09-26
O2k-Network
Kuala Lumpur MY, 2016 Sep 26-30. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC117.
MiPNet21.09 IOC113 Havana CU2016-08-08
O2k-Network
Havana CU, 2016 Aug 08-09. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC113. O2k-Network Lab Â» CU Havana Pardo Andreu GL
MitoFit Science Camp 2016 Kuehtai AT2016-07-07
MitoFit
Kuehtai AT, 2016 Jul 07-13. MitoFit Science Camp and IOC112.
MiPNet21.15 IOC112 Kuehtai AT2016-07-07
O2k-Network
Kuehtai AT, 2016 Jul 07-13. MitoFit Science Camp and IOC112.
Long Night of Research 2016 Innsbruck AT2016-04-22
LNF.JPG
Innsbruck AT, 2016 Apr 22. OROBOROS at Long Night of Research, CCB. MitoFit – Training for the powerhouses of your blood- and muscle cells.
MiPNet21.01 IOC109 Schroecken AT2016-04-03
OROBOROS-solo.png
Schroecken AT, 2016 Apr 03-08. OroborosO2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC109.
Expert/inn/en-Workshop Medizintechnik Innsbruck AT2016-02-29
MitoFit
Innsbruck AT, 2016 Feb 29. Expert/inn/en-Workshop Medizintechnik
MitoFit Workshop en.co.tec medical device 2016-02-042016-02-04
MitoFit
Innsbruck AT, 2016 Feb 04. MitoFit Workshop en.co.tec medical device.
MitoFit Workshop Blood Cells 2016-01-082016-01-08
MitoFit
Innsbruck AT, 2016 Jan 08. MitoFit Workshop on blood cell respirometry for mitochondrial research and diagnostic applications.
MiPNet20.10 IOC106 Schroecken2015-10-06
OROBOROS-solo.png
Schroecken AT, 2015 Oct 06-11. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC106.
MiPNet20.16 IOC105 Riga2015-08-27
O2k-Network
Riga LV, 2015 Aug 27. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - innovabalt, IOC105.
MiPNet20.05 IOC104 Greenville2015-08-09
O2k-Network
Greenville NC US, 2015 Aug 09-10. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC104.
QualitÀtszirkel 20152015-06-30
MitoFit
Innsbruck AT, 2015 Jun 30. QualitÀtszirkel Programm pdf
Wissenschaft im Gespraech2015-06-10
MitoFit
Bregenz AT, 2015 Jun 10. Wissenschaft im GesprÀch: Erich Gnaiger, Zellforscher. Wissenschaft im GesprÀch


Dissemination

MitoFit press releases


MitoFit on the radio

Transmission:
- 6.7.2015, 21:00 to 22:00
- 9.8.2015, 11.00 to 12.00


MitoFit news by Oroboros

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