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MitoEAGLE protocols, terminology, documentation

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


MitoEAGLE protocols, terminology, documentation



WG1

Standard operating procedures and user requirement document: Protocols, terminology, documentation


MITOEAGLE Working Groups

WG1 Description

The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.

Respirometric reference protocols

A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
MITOEAGLE Proficiency ring test

Interlaboratory proficiency test

Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.

Instrumental platforms

Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.

Nomenclature

Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.

Knowledge management system

With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.

WG1 Participants

CountryCityContact
ArmeniaYerevanTrchounian K
AustraliaQueenslandHolland Olivia J
AustraliaMelobourneBotella Javier
AustraliaNedlandsPerks Kara L
AustraliaSouthportVidimce Josif
AustraliaBurwoodTrewin Adam J
AustraliaMelbourneWhitfield Jamie
AustraliaMelbourneStepto Nigel K
AustraliaNewtown, SydneyStocker Roland
AustraliaGeelong, VictoriaMcKenzie Matthew
AustraliaMelbourne, VICMuccini Anna Maria
AustraliaParkvilleHardee Justin P
AustraliaMelbourneSaner Nicholas
AustraliaQueenslandDong Lan-Feng
AustraliaMelbourneFerri Alessandra
AustraliaVictoriaVan Bergen Nicole J
AustraliaVictoriaWinwood-Smith Hugh
AustraliaSyndeyTon Riccardo
AustraliaMelbourneHewakapuge Sudinna
AustraliaMelbourneKuang Jujiao
AustraliaMelbourneLionett S
AustraliaClayton VictoriaAlton Lesley
AustraliaMelbourneCoughlan Melinda T
AustraliaSydneyHoehn Kyle L
AustraliaMelbourneCassar Samantha
AustraliaMelbourneGenders Amanda J
AustraliaNedlandsFilipovska Aleksandra
AustraliaQueenslandRadenkovic Filip
AustraliaQueenslandFisher Joshua J
AustraliaClaytonWolff Jonci Nikolai
AustraliaSydneyKrycer James R
AustriaInnsbruckPlangger Mario
AustriaInnsbruckSobotka Ondrej
AustriaInnsbruckThapa Maheshwor
AustriaInnsbruckMeszaros Andras T
AustriaInnsbruckSchartner Melanie
AustriaInnsbruckGarcia-Souza Luiz F
AustriaInnsbruckGoebel G
AustriaInnsbruckBergmeister Lisa
AustriaInnsbruckDi Marcello Marco
AustriaInnsbruckLaner Verena
AustriaInnsbruckAntunes Diana
AustriaInnsbruckKlocker Helmut
AustriaKapferer Werner
AustriaInnsbruckGnaiger Erich
AustriaInnsbruckGallee Leon
AustriaInnsbruckKarabatsiakis Alexander
AustriaInnsbruckPerez Valencia Juan Alberto
AustriaInnsbruckSchmitt Sabine
AustriaInnsbruckSchwarzer Christoph
AustriaAldransHaider Markus
AustriaInnsbruckCardoso Luiza HD
AustriaInnsbruckPassrugger Manuela
AustriaSalzburgKofler B
AustriaInnsbruckCecatto Cristiane
AustriaKlosterneuburgSazanov Leonid A
AustriaInnsbruckNindl Veronika
AustriaViennaKozlov Andrey V
AustriaInnsbruckBurtscher Martin
AustriaInnsbruckHoppel Florian
AustriaInnsbruckKeller Markus A
AustriaInnsbruckDoerrier Carolina
BelgiumLeuven, FlandersBaekelandt V
BelgiumNamurArnould Thierry
BelgiumLiegeVotion Dominique-Marie
BelgiumBrusselsSonveaux P
BelguimSart Tilman (Liège)Kruse C
BrasilPorto AlegreHaas Clarissa
BrasiliaLimeiraRopelle Eduardo R
BrazilRio de JaneiroGalina Antonio
BrazilSão PauloCrispim Marcell
BrazilSao PauloVercesi Anibal E
BrazilRio de JaneiroVieyra Adalberto
BrazilSanta Maria - RSGoncalves Debora Farina
BrazilSanta Maria - Rio Grande do SulSoares Felix Alexandre Antunes
BrazilSão PauloKowaltowski Alicia J
BrazilJaboticabalOliveira Marcos Tulio
BrazilSao PauloSilber Ariel Mariano
BrazilRio de JaneiroOliveira Marcus F
CanadaMontreal, QuebecBergdahl Andreas
CanadaOttawaHarper Mary-Ellen
CanadaEdmonton (Alberta)Lemieux Helene
CanadaEdmontonZaugg Michael
CanadaRimouskiMunro Daniel
CanadaSaint JohnPulinilkunnil Thomas
CanadaEdmontonHan Woo Hyun
CanadaVancouver, BCTausan Daniel
CanadaEdmontonLucchinetti Eliana
CanadaQuebecJoseph Vincent
CanadaWinnipegChowdhury Subir Roy
CanadaHamiltonTarnopolsky Mark A
CanadaGuelphHolloway Graham P
CanadaCharlottetownAdiele Reginald C
CanadaRimouskiBlier Pierre U
CanadaOttawaDarveau Charles-A
CanadaAntigonishWeir M
CanadaVancouverBovard Josh
CanadaMontréalBreton Sophie
CanadaMoncton, NBPichaud Nicolas
CanadaHamiltonScott Graham R
ChileCoyhaiqueJana Prado Fabian
ChinaXi`anLiu Jiankang
ChinaTianjinZhang Yong
ChinaPukou, Nanjing,Gan Zhenji
ChinaFuzhou, Fujian ProvinceChen Qi
CroatiaSplitLjubkovic M
CroatiaZagrebSaric A
CroatiaZagrebCurik I
CroatiaZagrebRubelj Ivica
Czech RepublicPlzen -Severni predmestiJedlicka Jan
Czech RepublicCeske BudejoviceZikova Alena
Czech RepublicPardubiceMajtnerova Pavlina
Czech RepublicPardubiceHandl Jiri
Czech RepublicPraha 2Danhelovska Tereza
Czech RepublicHradec KraloveStankova Pavla
Czech RepublicHradec KraloveCervinkova Zuzana
Czech RepublicVestecEzrova Zuzana
Czech RepublicPraha 2Zdrazilova Lucie
Czech RepublicVestecRohlena Jakub
Czech RepublicPardubiceRousar Tomas
Czech RepublicPragueDrahota Zdenek
Czech RepublicVestecDavidova Eliska
Czech RepublicPragueKrizova J
Czech RepublicPragueHoustek Josef
Czech RepublicHradec KraloveEndlicher Rene
Czech RepublicPrague 10Urban Tomas
Czech RepublicHradec KraloveKucera Otto
Czech repHradec KraloveFalaye T
DenmarkCopenhagenDela Flemming
DenmarkCopenhagenNielsen B
DenmarkAarhus NBoetker Hans Erik
DenmarkHvidovreNehlin Jan O
DenmarkCopenhagen NScheibye-Knudsen Morten
DenmarkCopenhagenLarsen Steen
DenmarkCopenhagenSoendergaard SD
DenmarkCopenhagen NMaise Chroeis K
DenmarkCopenhagenGonzalez-Franquesa Alba
DenmarkCopenhagenKraunsoee R
DenmarkRoskildeDalgaard Louise T
DenmarkAarhus NJespersen Nichlas Riise
DenmarkCopenhagen NMortensen OH
EgyptCairoAbdel-Rahman Engy Ali
EgyptCairoAli Sameh S
EstoniaTallinnPuurand Marju
EstoniaTallinnKaambre Tuuli
EstoniaTallinnKlepinin Aleksandr
EstoniaTallinnLaasmaa Martin
EstoniaTallinnTepp Kersti
EstoniaTallinnShevchuk Igor
EstoniaTallinnKarro N
EstoniaTallinnTruu Laura
EstoniaTallinnKlepinina Lyudmila
EstoniaTallinnVendelin Marko
EstoniaTartuPaju Kalju
FinlandHelsinkiSuomalainen Wartiovaara Anu
FinlandTurkuStier Antoine
FinlandHelsinkiJackson Christopher Benjamin
FinlandJyvaeskylaeKainulainen Heikki
FranceGueguen Naig
FranceBordeauxSarlak Saharnaz
FranceParisBouillaud Frederic
FranceAngersProcaccio Vincent
FranceParisArmand Anne-Sophie
FrancePlouzanéSalin Karine
FranceMaisons-Alfort CedexProla Alexandre
FranceToursDumas Jean-Francois
FranceBordeaux cedexMourier A
FranceNouzillyCollin-Chenot Anne
FranceAngersSpinazzi Marco
FranceMontpellierWrutniak-Cabello Chantal
FranceBordeaux cedexRossignol Rodrigue
FranceLilleMontaigne David
FranceMartiniqueCano Sanchez Maria Consolacion
FranceMartiniqueNeviere Remi
FranceBordeauxAmoedo Nivea Dias
GermanyGiessenSommer Natascha
GermanyCologneHo Dieu Hien
GermanyUlmSchaefer PM
GermanyGiessenSchulz Rainer
GermanyMainzMethner Axel
GermanyJenaSzibor Marten
GermanyDuesseldorfJelenik T
GermanyHannoverDas Anibh Martin
GermanyBremerhavenMark Felix Christopher
GermanyCologneMaciej Sarah
GermanyColognePesta Dominik
GermanyMunichZischka Hans
GermanyMagdeburgSchoenfeld Peter
GermanyMarburgVogt Sebastian
GermanyDuesseldorfPiel Sarah
GermanyGießenGrewal Rekha
GermanyFrankfurtHamann Andrea
GermanyCologneWiesner Rudolf J
GermanyWickert Anika
GermanyRegensburgRenner-Sattler Kathrin
GermanyGießenSilaidos Carmina
GermanyMarburgRamzan Rabia
GermanyFreiburgSchuele R
GermanyWilhelmshavenSalmon Pablo
GermanyCologneTrifunovic Aleksandra
GermanyDuesseldorfHaendeler Judith
GermanyDoermann Niklas
GermanyFrankfurt am MainViel Christian
GermanyRostockSokolova Inna
GermanyMainzMaull Felicia
GermanyDüsseldorfGoy C
GermanyFrankfurtOsiewacz Heinz D
GermanyDuesseldorfRoden Michael
GermanyNeuherbergEiner C
GermanyBiberach an der RißNold V
GermanyTuebingenKappler Lisa
GermanyFrankfurtWarnsmann Verena
Greece15771Andreadou Ioanna
GreeceThessalonikiLazou Antigone
GreeceAthensTrougakos Ioannis P
GreeceHeraklionTavernarakis Nektarios
GreeceAthensGumeni Sentiljana
HungaryBudapestHorvath G
HungaryDebrecenVerebne Tar K
HungaryBudapestChinopoulos Christos
HungarySzegedFerdinandy Peter
HungaryBudapestTretter Laszlo
HungaryBudapestKomlodi Timea
HungarySzegedBoros Mihaly
IndiaLucknowGayen Jiaur
IndiaHaryanaChakrabarti Sasanka
IndiaKollamSuravajhala Prashanth
IndiaVaranasiSonkar Vijay K
IndiaNew DelhiThakkar Himani
IndiaNew DelhiVincent Vinnyfred
IndiaHyderabadJha Rajan Kumar
IranTehranShirazi Reza
IranTehranSafaei Zahra
IrelandDublinPorter Richard K
IrelandDublinO'Gorman Donal
IsraelRishon Le ZionHachmo Yafit
IsraelZrifinSova Marina
IsraelBeer ShevaMishmar Dan
IsraelJerusalemSaada Reisch Ann
ItaliaUdineSalvadego Desy
ItalienPadova PDSzabo Ildiko
ItalyCampobassoPallotta Maria Luigia
ItalyBolognaGenova Maria Luisa
ItalyRomeScatena Roberto
ItalySt. LorenzenHarrison David K
ItalyMilanoBottoni P
ItalyAnconaBattino Maurizio
ItalyBolognaPaterlini S
ItalyUdineGrassi B
ItalyBolognaPorcelli AM
ItalyPadovaViscomi Carlo
ItalyMilanClementi Emilio
ItalyCataniaMessina Angela
ItalyBolognaGasparre G
ItalyPadovaBernardi Paolo
ItalyBolzanoZanon Alessandra
ItalyBolzano/BozenVolani Chiara
ItalyCataniaDe Pinto Vito
ItalyPadovaFernandez-Vizarra Erika
ItalyMonserratoIsola Raffaella
ItalyBariAttimonelli M
ItalyCataniaMagri Andrea
JapanSapporoMatsumoto J
JapanItabashi-ku TokyoTanaka Masashi
KitzbühelInnsbruckFischer Michael J
KoreaSeoulPak Youngmi Kim
KoreaIncheonKwak Hyo Bum
Korea (South)SeoulLee Hong Kyu
LatviaRigaSvalbe Baiba
LatviaRigaZvejniece Liga
LatviaRigaStelfa Gundega
LatviaRigaLiepins Edgars
LatviaRigaVolska Kristine
LatviaRigaInashkina I
LatviaRigaJansone Baiba
LatviaRigaDambrova Maija
LatviaRigaVilks Karlis
LuxembourgStrassenDevaux Yvan
MalaysiaKuala LumpurHassan Hazirah
MaltaMsidaVella Joanna
MexicoMexico CityAparicio Trejo OE
MexicoTlalpanRodriguez-Enriquez Sara
MéxicoSan Pedro Garza García, Nuevo LeonGarcia-Rivas Gerardo
NetherlandsMaastrichtGrevendonk L
NetherlandsGA WageningenGrefte Sander
New ZealandAucklandHickey Anthony J
New ZealandWellingtonBerridge Michael V
New ZealandAucklandKaur Sarbjot
New ZealandAucklandWard Marie Louise
NorwayTrondheimLerfall Joergen
NorwayBergenRoesland Gro Vatne
NorwayBergenPettersen Nitschke Ina Katrine
NorwayBergenHoel Fredrik
NorwayTrondheimGrill V
NorwayBergenTronstad Karl Johan
NorwayBergenBerge Rolf K
NorwayTromsoeBoardman Neoma T
NorwayBergenBlindheim Dan Filip
NorwayOsloEide L
NorwayBergenHaavik Jan
NorwayTrondheimSmenes Benedikte Therese
NorwayTrondheimRognmo O
NorwayBergenDyrstad Sissel E
PolandLodzLabieniec-Watala Magdalena
PolandPoznanJarmuszkiewicz Wieslawa
PolandGdańskKaczor Jan Jacek
PolandWarsawSzewczyk Adam
PolandWarsawSek Aleksandra
PolandWarsawKarkucinska-Wieckowska Agnieszka
PolandWarsawBednarczyk Piotr
PolandWarsawWasilewski M
PolandWarsawWieckowski Mariusz R
PolandWarsawKampa Rafal Pawel
PolandWarsawZablocki Krzysztof
PolandWarszawaJaskiewicz Anna
PolandKrakowDembinska-Kiec Aldona
PolandLodzWatala Cezary
PolandWarsawDymkowska Dorota
PortugalPortoMoreira Bruno P
PortugalCoimbraSantos Diana
PortugalPortoSilva Ana Maria
PortugalAveiroVitorino Rui Miguel Pinheiro
PortugalCantanhedeAmorim Ricardo
PortugalCoimbraDias Candida
PortugalPortoBeleza Jorge
PortugalCoimbraDuarte Filipe Valente
PortugalPortoAlves Marco G
PortugalCoimbraRolo Anabela Pinto
PortugalCoimbraOliveira Paulo J
PortugalCoimbraGrilo Luis
PortugalCoimbraMinuzzi Luciele M
PortugalLisboaMacedo Maria Paula
PortugalAveiroFerreira Rita Maria P
PortugalPortoOliveira Jorge
PortugalCoimbraPalmeira Carlos
PortugalPortoOliveira Pedro Fontes
PortugalCantanhedeTeodoro Joao Soeiro
PortugalCantanhedeRodrigues Ana Sofia
PortugalPortoCrisostomo Luis
QatarDohaGuarch Meritxell Espino
Republic of GhanaCape CoastTei BN
Republic of KoreaBusanKim Hyoung Kyu
Republic of KoreaBusanHan Jin
Republic of KoreaYuseong-gu DaejeonQuang Don T
Republic of KoreaDaejeonPark SH
Republic of SerbiaBelgradeLalic Nebojsa M
Republic of SerbiaNovi SadAndric Silvana
Republic of SerbiaBelgradeKrako Jakovljevic Nina
RussiaYoshkar-OlaDubinin M
RussiaMoscowVinogradov Andrey D
RussiaMoscowPavlova Nadia
SerbiaBelgradeSavkovic U
SerbiaBelgradeMisirkic Marjanovic Maja
SerbiaBelgradeMandic M
SerbiaBelgradeDjordjevic M
SingaporeSingaporeHausenloy Derek J
SingaporeSingaporeSingh Brijesh Kumar
SlovakiaBratislava 45Ferko Miroslav
SlovakiaKošiceCizmarova Beata
SlovakiaBratislavaSumbalova Zuzana
SpainBarcelonaBravo-Sagua Roberto
SpainA CoruñaBlanco FJ
SpainGranadaHidalgo-Gutierrez A
SpainMadridMoran M
SpainValenciaCasado Pinna Marta
SpainBarcelonaGil J
SpainBarcelonaGama Perez Pau
SpainGranadaQuiles JL
SpainBarcelonaGarcia-Roves Pablo Miguel
SpainSevillaBustos Matilde
SpainBarcelonaZorzano Antonio
SpainMadridHernansanz-Agustin Pablo
SpainBarcelonaDe la Torre Lara J
SpainA CoruñaMayan MD
SpainSalamancaAlmeida Angeles
SpainMadridAragones Lopez J
SpainArmilla (Granada)Fernandez-Ortiz Marisol
SpainLas Palmas de Gran CanariaCalbet Jose AL
SpainBarcelonaGoncalo Teixeira da Silva Rui
SpainMadridRial Eduardo
SpainBarcelonaPerales Jose Carles
SwedenStockholmCardinale Daniele A
SwedenStockholmNedergaard J
SwedenStockholmLarsen Filip J
SwedenStockholmShabalina Irina G
SwedenGothenburgBhuvanachandran NS
SwedenLundEhinger Johannes K
SwitzerlandLausanneGebara E
SwitzerlandBaselBouitbir Jamal
SwitzerlandBernWyss RK
SwitzerlandBaselFornaro Mara
SwitzerlandLausanneLagarrigue Sylviane
SwitzerlandZurichGorr Thomas A
SwitzerlandLausanneMorato Fornaguera L
SwitzerlandLausannePlace Nicolas
SwitzerlandBernNuoffer Jean-Marc
SwitzerlandLausanneDonnelly Chris
SwitzerlandZürichZaugg Kathrin
SwitzerlandLausanneWeger M
SwitzerlandLausanneZanou Nadege
SwitzerlandLausanneBurtscher Johannes
SwitzerlandLausanneZalachoras I
SwitzerlandLausanneAmati Francesca
SwitzerlandLausanneCanto Alvarez Carles
SwitzerlandLausanneSandi Carmen
TaiwanChanghua CityWei Yau-Huei
The NetherlandsMaastrichtNabben Miranda
The NetherlandsBT AmsterdamWuest Rob CI
The NetherlandsGroningenBakker Barbara M
The NetherlandsMaastrichtSchrauwen Patrick
The NetherlandsDelftMcMillan Duncan GG
UKCanterbury, KentGourlay Campbell W
UKLondonLane Nick
UKLondonRodriguez Enrique
UKLondonBettinazzi Stefano
UKCambridgeBrown Guy C
USAWakefieldVarricchio Frederick
USACambridgeWohlwend Martin
USAFort CollinsLi Puma Lance C
USANeedhamBrown David A
USALittle RockParajuli Nirmala
USAEast LansingBazil Jason N
USAMiamiFontanesi F
USAKansas CityThyfault John P
USABostonKristal Bruce S
USANew HavenCavalcanti-de-Albuquerque Joao Paulo
USAPhiladelphiaTomar Dhanendra
USAChicagoDayanidhi Sudarshan
USAIndianapolisBrozinick Joseph T
USANew YorkGalkin Alexander
USAFort CollinsChicco Adam J
USACorvallisBatterson Philip M
USAAtlantaJang Young Charles
USATorranceRossiter Harry B
USAWinston-SalemGonzalez-Armenta Jenny L
USAClevelandHoppel Charles L
USASt. LouisAbumrad Nada A
USAFairbanksO'Brien Kristin
USAOrlandoSparks Lauren M
USAWinston-SalemAhn Bumsoo
USASan DiegoPatel Hemal H
USARochesterNair K Sreekumaran
USAIowa CityChaurasia Bhagirath
USABaltimoreGonzalez-Freire Marta
USAStillwaterDavis Michael S
USAGainesvilleLeeuwenburgh Christiaan
USABirminghamMoellering Douglas R
USASeattleMarcinek DJ
USAIowa CityWagner Brett A
USAPhiladelphiaGanetzky Rebecca
USAPittsburghJurczak Michael J
USAPhiladelphiaTowheed Atif
USASan Diego; La JollaMolina Anthony JA
USANorfolkLai Nicola
USAJupiterTrivigno Catherine
USASt. LouisPietka Terri A
USAEast LansingZhang Yizhu
USADavisHellgren Kim T
USAPhiladelphiaJang David H
USADavisAdams Sean H
USARochesterLanza Ian R
USAProvoHancock Chad R
USAAkronCohen Bruce H
USAPittsburghProchownik Edward V
USALos AngelesKwast Kurt E
USAAuroraFord Ellen
USAKansas CitySwerdlow Russell H
USASan DiegoCannon Daniel T
USAColorado SpringsJacobs RA
USAGalvestonDurham William J
USAAnn ArborTyrrell Daniel J
USACorvallisNewsom Sean A
USAFayettevilleIyer Shilpa
USAColumbusSims Carrie A
USAMilwaukeeDash Ranjan K
USADentonDzialowski Edward M
USAOrlandoCoen Paul M
USAOmahaKim Julian KS
USABaton RougeIrving Brian A
USABethesdaSharma Pushpa
USAPortlandGarlid Keith D
USADenverVan Hove Johan
USAFayettevilleRao RR
USABaton RougeHand Steven C
USAPuskarich Michael A
USAOrlandoGoodpaster Bret H
USAClevelandTandler Bernard
USADurhamLi Pingan Andy
USANashvilleWasserman David H
USABerkeleyWilliams Caroline M
USAAuroraSparagna Genevieve C
USAPhiladelphiaFalk Marni J
USAEl PasoBajpeyi Sudip
USAStanfordStary Creed
USALa JollaValentine Joseph Marco
USAFairbanksCoker Robert H
USAGreenvilleNeufer P Darrell
USACorvallisRobinson Matthew M
USAGainesvilleNeyroud D
USABethesdaFessel Joshua Patrick
... further results

WG1 Management

Tasks

  1. Opening of the database to the research public for data search and moderated entry of new data sets.
  2. Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
  3. Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
  4. Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
  5. Instrumental platform comparison with reference sample applying comparable measurements.
  6. Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
  7. Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
  8. MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
  9. MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
  10. Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
  11. Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
  12. Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
  13. Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).


Milestones

  1. Data sets entered in standardized format and database opened for the public
  2. Protocols for ring-tests and standard analysis and report scheme defined
  3. MITOEAGLE proficiency training workshop finished (move to WG5)
  4. Analysis is completed and joint publication submitted (with WG5)
  5. Publication of paper on concepts and terminology (with WG5)
  6. Library of protocols online
  7. Publication of recommended protocols and procedures
  8. Guidelines for acquisition, evaluation and data documentation available (with WG5)
  9. MITOEAGLE data management system finished
  10. MITOEAGLE database ready for use
  11. Software modifications incorporated following extensive tests
  12. Publication of MITOEAGLE-KMP
  13. Recommendations on data acquisition, evaluation and management published (with WG5)
  14. Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)


Deliverables

  1. Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
  2. A database on mt fitness evaluated with human cells and tissues
  3. Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
  4. Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
  5. An expanded MITOEAGLE database
  6. A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
  7. A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
  8. A library of protocols for sample preparation and examination by respirometry
  9. A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
  10. Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
  11. The MITOEAGLE data management system
  12. A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
  13. A joint publication on the use of the MITOEAGLE database and management system (with WG5)
  14. An education programme for the use of MITOEAGLE database and management system (move to WG5).

WG1 Feedback

  1. We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
  2. My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
  3. Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
    1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
    2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
    3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
  4. I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
  5. I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
    1. Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
    2. Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
    3. Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
  6. Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
    1. stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
    2. hypogonadal-hypogonadism -/+ testosterone-replacement therapy
    3. androgens anabolics treatment -/+ blockade od AR
    4. aged rats -/+ Viagra-treatment
    5. biological clock - Silvana Andric (2016).
  7. I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
  8. I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. - Maria Luisa Genova (2016).

Projects in progress

Interlaboratory proficiency ring test
» MitoFit proficiency test
Testing of software components
» MiPNet21.07 MitoFit DatLab proficiency test
Concepts and terminology on mitochondrial physiology
» MitoPedia: Respiratory states
» MitoPedia: Respiratory control ratios
» MitoPedia: SUIT
Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
» MitoPedia: Sample preparations
» MitoPedia: Media for respirometry
» MitoPedia: Substrates and metabolites
» MitoPedia: SUIT
» MiPNet21.06 SUIT reference protocol
» MitoFit-QCS
Data management system (DMS)
» MitoFit-KMP
Database
» MitoFit Quality Control System
» MiPMap


Next steps

Best practices for implementation of analytical and process standards.


References

» ISO/IEC 17043:2010 General requirements for proficiency testing
» ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison
» ISO 15189:2012 Medical laboratories — Particular requirements for quality and competence
» ISO 17511:2003 In vitro diagnostic medical devices
» ISO 9001:2015 Quality management systems - requirements
» ISO 10012:2003 Measurement management systems
General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005