Difference between revisions of "MitoEAGLE protocols, terminology, documentation"

Latest revision as of 12:05, 19 January 2018

News and Events

Working Groups

Short-Term Scientific Missions

Management Committee

Members

COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

MitoEAGLE protocols, terminology, documentation

WG1 Project application

Standard operating procedures and user requirement document: Protocols, terminology, documentation

» WG1 Action

The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.

Respirometric reference protocols

A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).

Interlaboratory proficiency test

Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MitoEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.

Instrumental platforms

Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.

Nomenclature

Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MitoEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.

Knowledge management system

With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.

The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MitoEAGLE Knowledge Management System.

Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.

It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MitoEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.

Management

Tasks

Opening of the database to the research public for data search and moderated entry of new datasets.
Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MitoEAGLE proficiency training module.
Sample distribution, ring test experiments (MitoEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MitoEAGLE-proficiency test manager.
Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
Instrumental platform comparison with reference sample applying comparable measurements.
Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
MitoEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
MitoEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
Provide a summary on strategic dissemination and an education programme for MitoEAGLE.

Milestones

Data sets entered in standardized format and database opened for the public
Protocols for ring-tests and standard analysis and report scheme defined
MitoEAGLE proficiency training workshop finished
Analysis is completed and joint publication submitted
Publication of paper on concepts and terminology
Library of protocols online
Publication of recommended protocols and procedures
Guidelines for acquisition, evaluation and data documentation available
MitoEAGLE data management system finished
MitoEAGLE database ready for use
Software modifications incorporated following extensive tests
Publication of MitoEAGLE-KMP
Recommendations on data acquisition, evaluation and management published
Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published

Deliverables

Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
A database on mt fitness evaluated with human cells and tissues
Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
An expanded MitoEAGLE database
A joint publication documenting the results of the MitoEAGLE-PT
A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
A library of protocols for sample preparation and examination by respirometry
A multi-authored publication with recommendations of comparable standard protocols and procedures.
Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
The MitoEAGLE data management system.
A continuously updated MitoEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
A joint publication on the use of the MitoEAGLE database and management system.
An education programme for the use of MitoEAGLE database and management system.

@@ Line 2: / Line 2: @@
 __TOC__
 <br />
-<big><big><big>'''WG1'''
+<big><big><big>'''WG1 Project application'''
 :::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation'''
 </big></big></big>
+::::» [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]
+[[File:MITOEAGLE Working groups.jpg|right|400px|MitoEAGLE Working Groups]]
-[[File:MITOEAGLE Working groups.jpg|right|400px|MITOEAGLE Working Groups]]
-== WG1 Description ==
 :::: The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
@@ Line 14: / Line 13: @@
 === Respirometric reference protocols ===
-:::: A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
+:::: A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).
-[[File:MITOEAGLE Proficiency ring test.jpg|right|400px|MITOEAGLE Proficiency ring test]]
+[[File:MITOEAGLE Proficiency ring test.jpg|right|400px|MitoEAGLE Proficiency ring test]]
 === Interlaboratory proficiency test ===
-:::: Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the [[ISO/IEC 17043:2010 General requirements for proficiency testing |ISO 17043 and 13528]] standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
+:::: Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the [[ISO/IEC 17043:2010 General requirements for proficiency testing |ISO 17043 and 13528]] standards. A corresponding MitoEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
 === Instrumental platforms ===
@@ Line 24: / Line 23: @@
 === Nomenclature ===
-:::: Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
+:::: Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MitoEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
 === Knowledge management system ===
@@ Line 30: / Line 29: @@
 :::: With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
-:::: The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
+:::: The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MitoEAGLE Knowledge Management System.
 :::: Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
-:::: It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
+:::: It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MitoEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
-== WG1 Participants ==
+== Management ==
-{{#ask:[[MitoPedia topic::EAGLE]] [[In country::+]] [[Has COST WG1::+]]
-| mainlabel=-
-|?In country=Country
-|?In city=City
-|?=Contact
-|sort=In country
-|order=ascending
-|limit=5000
-|format=broadtable
-|offset=0
-}}
-== WG1 Management ==
 === Tasks ===
-:::# Opening of the database to the research public for data search and moderated entry of new data sets.
+:::# Opening of the database to the research public for data search and moderated entry of new datasets.
-:::# Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (''with WG5'').
+:::# Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MitoEAGLE proficiency training module.
-:::# Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
+:::# Sample distribution, ring test experiments (MitoEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MitoEAGLE-proficiency test manager.
-:::# Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report  (''move to WG5'').
+:::# Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
 :::# Instrumental platform comparison with reference sample applying comparable measurements.
-:::# Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (''with WG5'').
+:::# Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
 :::# Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
-:::# MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
+:::# MitoEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
-:::# MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
+:::# MitoEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
 :::# Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
 :::# Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
-:::# Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation  (''move to WG5'').
+:::# Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
-:::# Provide a summary on strategic dissemination and an education programme for MITOEAGLE  (''move to WG5'').
+:::# Provide a summary on strategic dissemination and an education programme for MitoEAGLE.
@@ Line 69: / Line 55: @@
 :::# Data sets entered in standardized format and database opened for the public
 :::# Protocols for ring-tests and standard analysis and report scheme defined
-:::# MITOEAGLE proficiency training workshop finished (''move to WG5'')
+:::# MitoEAGLE proficiency training workshop finished
-:::# Analysis is completed and joint publication submitted (''with WG5'')
+:::# Analysis is completed and joint publication submitted
-:::# Publication of paper on concepts and terminology (''with WG5'')
+:::# Publication of paper on concepts and terminology
 :::# Library of protocols online
 :::# Publication of recommended protocols and procedures
-:::# Guidelines for acquisition, evaluation and data documentation available (''with WG5'')
+:::# Guidelines for acquisition, evaluation and data documentation available
-:::# MITOEAGLE data management system finished
+:::# MitoEAGLE data management system finished
-:::# MITOEAGLE database ready for use
+:::# MitoEAGLE database ready for use
 :::# Software modifications incorporated following extensive tests
-:::# Publication of MITOEAGLE-KMP
+:::# Publication of MitoEAGLE-KMP
-:::# Recommendations on data acquisition, evaluation and management published (''with WG5'')
+:::# Recommendations on data acquisition, evaluation and management published
-:::# Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (''move to WG5'')
+:::# Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published
 === Deliverables ===
-:::# Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (''with WG5'')
+:::# Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
-:::# A  database on mt fitness evaluated with human cells and tissues
+:::# A database on mt fitness evaluated with human cells and tissues
-:::# Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (''move to WG5'')
+:::# Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
 :::# Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
-:::# An expanded MITOEAGLE database
+:::# An expanded MitoEAGLE database
-:::# A joint publication documenting the results of the MITOEAGLE-PT (''with WG5'')
+:::# A joint publication documenting the results of the MitoEAGLE-PT
-:::# A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (''with WG5'')
+:::# A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
 :::# A library of protocols for sample preparation and examination by respirometry
-:::# A multi-authored publication with recommendations of comparable standard protocols and procedures (''with WG5'')
+:::# A multi-authored publication with recommendations of comparable standard protocols and procedures.
-:::# Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
+:::# Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
-:::# The MITOEAGLE data management system
+:::# The MitoEAGLE data management system.
-:::# A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
+:::# A continuously updated MitoEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
-:::# A joint publication on the use of the MITOEAGLE database and management system (''with WG5'')
+:::# A joint publication on the use of the MitoEAGLE database and management system.
-:::# An education programme for the use of MITOEAGLE database and management system (''move to WG5'').
+:::# An education programme for the use of MitoEAGLE database and management system.
-== WG1 Feedback ==
-::::# '' We are mostly interested in two WG: (1) Since we are working mostly  with heart and muscles, we are interested in WG 2 - MITOEAGLE data  repository in muscle and other tissues. (2) WG 1 - Standard operating  procedures and user requirement document: Protocols, terminology,  documentation. Since we have an expertise in fatty acid metabolism, we  are specially interested in experimental protocols for the evaluation of  mitochondrial capacities regarding fatty acid metabolism. Of course our  parts of the WG are also in our interest.'' - '''[[Makrecka-Kuka M| Marina Makrecka-Kuka]], [[Liepinsh E|Edgars Liepinsh]]''' (2016).
-::::# '' My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on  skeletal and cardiac muscle and cultured cardiomyocytes and endothelial  cells. I would particularly like to bridge the gap between the group  leaders and young investigators, trying to represent the young people  who are the active users of the OROBOROS equipment. This will fit in  nicely with the goals in WG1. ... Major congratulations on obtaining  this beautiful collaborative grant! Very glad to see this network  getting the recognition it deserves! Looking forward to being part of  this!'' - '''[[Wuest RC| Rob C Wuest]]''' (2016).
-::::# ''Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
-::::## ''Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
-::::## ''Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
-::::## ''Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - '''[[Watala C| Cezary Watala]]''' (2016).
-::::# ''I have already contacted the portuguese CNC to nominate me for  the MC of theaction. Concerning my active participation, taking into  account the type of work we do in my lab I could be included in all WGs.  As you know we work with mitochondria from liver, muscle and fat, as  well as cultured cells from different origins (WG2-4). The elaboration  of SOPs (WG1) we can certainly contribute (see the book I edited on  Mitochondrial Bioenergetics and in which you contribute a chapter). I  believe that everybody can be part of the WG5, but as the coordinators  of the action, you and Sandra should decide what is best for the success  of the action.'' - '''[[Palmeira C |Carlos Palmeira]]''' (2016).
-::::# ''I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
-::::##''Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
-::::##''Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
-::::##''Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory  ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU,  researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining  and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability.'' - '''[[Lalic Nebojsa|Nebojsa Lalic]]''' (2016).
-::::# ''Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:''
-::::## ''stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
-::::## ''hypogonadal-hypogonadism -/+ testosterone-replacement therapy
-::::## ''androgens anabolics treatment -/+ blockade od AR
-::::## ''aged rats -/+ Viagra-treatment
-::::## ''biological clock'' - '''[[Andric S|Silvana Andric]]''' (2016).
-::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation.  Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
-::::# ''I will be pleased to contribute to the tasks of WG1 by developing  interlaboratory proficiency tests and instrumental platform comparison.'' - '''[[Genova ML| Maria Luisa Genova]]''' (2016).
-== Projects in progress ==
-::: '''Interlaboratory proficiency ring test'''
-::::» [[MitoFit proficiency test]]
-::: '''Testing of software components'''
-::::» [[MiPNet21.07 MitoFit DatLab proficiency test]]
-::: '''Concepts and terminology on mitochondrial physiology'''
-::::» [[MitoPedia: Respiratory states]]
-::::» [[MitoPedia: Respiratory control ratios]]
-::::» [[MitoPedia: SUIT]]
-::: '''Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols'''
-::::» [[MitoPedia: Sample preparations]]
-::::» [[MitoPedia: Media for respirometry]]
-::::» [[MitoPedia: Substrates and metabolites]]
-::::» [[MitoPedia: SUIT]]
-::::» [[MiPNet21.06 SUIT reference protocol]]
-::::» [[MitoFit-QCS]]
-::: '''Data management system (DMS)'''
-::::» [[MitoFit-KMP]]
-::: '''Database'''
-::::» [[MitoFit Quality Control System]]
-::::» [[MiPMap]]
-=== Next steps ===
-::: '''Best practices''' for implementation of analytical and process standards.
-=== References ===
-::::» [[ISO/IEC 17043:2010 General requirements for proficiency testing]]
-::::» [[ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison]]
-::::» [[ISO 15189:2012 Medical laboratories — Particular requirements for quality and competence]]
-::::» [[ISO 17511:2003 In vitro diagnostic medical devices]]
-::::» [[ISO 9001:2015 Quality management systems - requirements]]
-::::» [[ISO 10012:2003 Measurement management systems]]
-:::: General requirements for the competence of testing and calibration laboratories » [http://www.iso.org/iso/catalogue_detail.htm?csnumber=39883 ISO/IEC 17025:2005]
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