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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Gnaiger Erich |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Arnould Thierry |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Renard P |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen | Desler C |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Aarhus | Rattan Suresh IS |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Klepinina Lyudmila |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Helsinki | Pirinen E |
| Finland | Turku | Stier Antoine |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| Germany | Ulm | Hartmann C |
| Germany | Munich | Jastroch M |
| Germany | Hamburg | Singer Dominique |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Freising | Schoettl T |
| Germany | Ulm | Kolassa Iris |
| Germany | Biberach an der Riß | Nold V |
| Germany | Ulm | Calzia Enrico |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Gießen | Silaidos Carmina |
| Germany | Duesseldorf | Eckers A |
| Germany | Freising | Fromme Tobias |
| Germany | Ulm | Schaefer PM |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Freising | Klingenspor Martin |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Leipzig | Ost Mario |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Freising | Keppner Gloria |
| Germany | Giessen | Eckert Gunter P |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Dublin | Porter Richard K |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Bologna | Porcelli AM |
| Italy | Ancona | Battino Maurizio |
| Italy | Bari | Agrimi G |
| Italy | Milano | Bottoni P |
| Italy | Bologna | Gasparre G |
| Italy | Rome | Scatena Roberto |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Nijmegen | Koopman Werner JH |
| Netherlands | Groningen | Rots MG |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Lodz | Watala Cezary |
| Poland | Poznań | Ludziejewska A |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Poznan | Swiniuch Daria |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Sek Aleksandra |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Covilhã | Dias Tania R |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Danila Maria-Daniela |
| Russia | Moscow | Krylova T |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrade | Vukojevic M |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Maderova D |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Madrid | Cadenas S |
| Spain | Madrid | Monsalve Maria |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | Lopez MG |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Arago Marc |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Smolders V |
| Spain | Barcelona | Gil J |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Granada | Quiles JL |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Barcelona | Juarez D |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | A Coruña | Mayan MD |
| Spain | Madrid | De los Rios C |
| Spain | Valencia | Borras Consuelo |
| Spain | Lleida | Nogueras L |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Madrid | Cano-Abad MF |
| Sweden | Lund | Chamkha Imen |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Westerlund E |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Elmer Eskil |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Izmir | Aydin HH |
| Turkey | Istanbul | Baykal AT |
| USA | Philadelphia | Starr Jonathan |
| USA | Fayetteville | Rao RR |
| USA | St Louis | Welch R |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Philadelphia | Janowska Joanna |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| USA | Fayetteville | Iyer Shilpa |
| USA | Iowa City | Buettner Garry R |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
