Difference between revisions of "MitoEAGLE data: blood and cultured cells"
From Bioblast
| Line 14: | Line 14: | ||
== WG4 Participants == | == WG4 Participants == | ||
{{#ask:[[MitoPedia topic::EAGLE]] [[In country::+]] [[Has COST WG4::+]] | |||
| mainlabel=- | | mainlabel=- | ||
|?In country=Country | |?In country=Country | ||
Revision as of 14:58, 27 April 2016
 |
News and Events |  |
 |
Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Hoppel Florian |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Detraux Damien |
| Belgium | Namur | Arnould Thierry |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Renard P |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Copenhagen | Desler C |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Copenhagen N | Mortensen OH |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Klepinina Lyudmila |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| Finland | Jyvaeskylae | Lehti Maarit |
| France | Bron | Rizwan Alam M |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| Germany | Leipzig | Ost Mario |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Freising | Keppner Gloria |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Ulm | Hartmann C |
| Germany | Giessen | Eckert Gunter P |
| Germany | Munich | Jastroch M |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Hamburg | Singer Dominique |
| Germany | Ulm | Kolassa Iris |
| Germany | Freising | Schoettl T |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Biberach an der Riß | Nold V |
| Germany | Ulm | Calzia Enrico |
| Germany | Duesseldorf | Eckers A |
| Germany | Freising | Fromme Tobias |
| Germany | Ulm | Schaefer PM |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Freising | Klingenspor Martin |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Ireland | Dublin | Porter Richard K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Zrifin | Sova Marina |
| Israel | Jerusalem | Saada Reisch Ann |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Ancona | Battino Maurizio |
| Italy | Bari | Agrimi G |
| Italy | Bologna | Porcelli AM |
| Italy | Milano | Bottoni P |
| Italy | Rome | Scatena Roberto |
| Italy | Bologna | Gasparre G |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Groningen | Rots MG |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Oslo | Lund Jenny |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Lodz | Watala Cezary |
| Poland | Poznań | Ludziejewska A |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Poznan | Swiniuch Daria |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Lighezan R |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Vukojevic M |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropcova B |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Spain | Lleida | Nogueras L |
| Spain | Madrid | De los Rios C |
| Spain | Valencia | Borras Consuelo |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Madrid | Cano-Abad MF |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | Cadenas S |
| Spain | Madrid | Monsalve Maria |
| Spain | Barcelona | Tura Ceide O |
| Spain | Madrid | Lopez MG |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Smolders V |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Gil J |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Juarez D |
| Spain | A Coruña | Mayan MD |
| Spain | Armilla, Granada | Escames Germaine |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden | Lund | Chamkha Imen |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Westerlund E |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Elmer Eskil |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| USA | Philadelphia | Starr Jonathan |
| USA | Fayetteville | Rao RR |
| USA | St Louis | Welch R |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Philadelphia | Janowska Joanna |
| USA | Orlando | Coen Paul M |
| USA | Baton Rouge | Irving Brian A |
| USA | Fayetteville | Iyer Shilpa |
| USA | Iowa City | Buettner Garry R |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
