Difference between revisions of "MiPNet20.14 AmplexRed H2O2-production"
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|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/5/57/MiPNet20.14_AmplexRed_H2O2-production.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:MiPNet20.14_AmplexRed_H2O2-production.pdf Versions] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/5/57/MiPNet20.14_AmplexRed_H2O2-production.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:MiPNet20.14_AmplexRed_H2O2-production.pdf Versions] | ||
|authors=Oroboros | |authors=Oroboros | ||
|year=2018-11- | |year=2018-11-26 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Krumschnabel G, Fontana-Ayoub M, Fasching M, Gnaiger E (2018) O2k-Fluorometry: HRFR and simultaneous determination of H<sub>2</sub>O<sub>2</sub> production with Amplex UltraRed. Mitochondr Physiol Network 20.14(03):1-6.''' | |abstract='''Krumschnabel G, Fontana-Ayoub M, Fasching M, Gnaiger E (2018) O2k-Fluorometry: HRFR and simultaneous determination of H<sub>2</sub>O<sub>2</sub> production with Amplex UltraRed. Mitochondr Physiol Network 20.14(03):1-6.''' |
Revision as of 02:38, 26 November 2018
O2k-Fluorometry: HRFR and simultaneous determination of H2O2 production with Amplex UltraRed. |
» »Versions
Oroboros (2018-11-26) Mitochondr Physiol Network
Abstract: Krumschnabel G, Fontana-Ayoub M, Fasching M, Gnaiger E (2018) O2k-Fluorometry: HRFR and simultaneous determination of H2O2 production with Amplex UltraRed. Mitochondr Physiol Network 20.14(03):1-6.
High-Resolution FluoRespirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels. Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
- Quality control of preparations by respirometric performance.
- Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
- Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
- Additional information is acquired for a limited amount of sample.
- Oxygen dependence of parameters can be studied.
In the publication by Krumschnabel et al. 2015 we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H2O2 production of mouse brain mitochondria. The paper by Makrecka-Kuka et al. 2015 focuses on the application of the Amplex red assay for determining H2O2 production in three different models of mitochondrial preparations.
O2k-Publication:
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. »Bioblast link«
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. »Bioblast link«
• O2k-Network Lab: AT_Innsbruck_Oroboros
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart
Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: S
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor, MitoFitPublication