MiPNet19.18E O2 flux analysis - DatLab 6

Gnaiger E. Oxygen flux analysis: DatLab On-Line. Mitochondr Physiol Network 12.09.

» MiPNet12.09

Gnaiger E (

) Mitochondr Physiol Network

Abstract: DatLab 4 sets a novel standard in high-resolution respirometry, for on-line analysis of oxygen flux measured in the OROBOROS Oxygraph-2k, and of other signals obtained in the O2k- MultiSensor System. Oxygen flux can be plotted with or without O2kbackground correction, is instantaneously expressed per mass of sample or per number of cells. Various sections on the plot of oxygen flux are marked, and corresponding average values are viewed in a table which can be simply exported into Excel or SigmaPlot. Instrumental and experimental parameters are summarized in a protocol, which can be printed or saved as a pdf file. These new features provide the basis for combining high-resolution with instant and user-friendly analysis. A demonstration experiment, performed during an Oxygraph-2k Workshop on high-resolution respirometry, is used as an example for application of DatLab 4 and DatLab-Excel templates MiPNet08.09. All analyses can be performed on-line or off-line. • Keywords: HRR-DatLab

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels:

Coupling state: OXPHOS 

HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-SOP 

Info

MiPNet09.09, MiPNet08.08 and MiPNet10.01 is integrated in MiPNet12.09_O2FluxAnalysis.