Difference between revisions of "MiPNet17.03 Shredder vs Fibers"

Latest revision as of 16:58, 9 December 2020

MiPNet17.03 Shredder vs Fibers

Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.

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Oroboros (2015-06-18) Mitochondr Physiol Network

Abstract: Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E (2015) Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03(04):1-9.

In the present study we compared mitochondrial function of permeabilized fibers and homogenate of heart muscle of mice. In addition, respiration of trout heart homogenate preparations were compared with permeabilized fibers, and the PBI-Shredder was successfully tested with preparation of trout liver.

» Product: Oroboros O2k, O2k-Catalogue

• Keywords: Tissue homogenate, Shredder

• O2k-Network Lab: AT_Innsbruck_Oroboros, AT Innsbruck MitoCom

Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP

Organism: Fishes Tissue;cell: Heart, Liver Preparation: Homogenate

Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Protocol

O2k-Demo, Mt-Preparation, Auxiliary, PBI-Shredder, O2k-Core

Further information

» PBI-Shredder O2k-Set

@@ Line 1: / Line 1: @@
+{{OROBOROS header page name}}
 {{Publication
-|title=Draxl A, Eigentler A, Gnaiger E. Mitochondrial respiration in permeabilized fibres versus homogenate from mouse
-myocardium. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
-|info=[[PBI-Shredder HRR-Set]]
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/8/80/MiPNet17.03_PBI-Shredder_Fish-heart-liver.pdf|Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet17.03_PBI-Shredder_Fish-heart-liver.pdf Versions]
-|authors=Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E
+|authors=Oroboros
-|year=2012
+|year=2015-06-18
 |journal=Mitochondr Physiol Network
-|abstract=In the present study we compared mitochondrial function of permeabilized fibres and homogenate of heart muscle of mice. In addition, respiration of trout heart homogenate preparations were compared with permeabilized fibres, and the PBI-Shredder was successfully tested with preparation of trout liver.
+|abstract='''Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E (2015) Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03(04):1-9.'''
-|keywords=Tissue homogenate, shredder
-|mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E
+In the present study we compared mitochondrial function of permeabilized fibers and homogenate of heart muscle of mice. In addition, respiration of trout heart homogenate preparations were compared with permeabilized fibers, and the PBI-Shredder was successfully tested with preparation of trout liver.
-|articletype=Protocol; Manual, MiPNet-online Publication
+:» Product: [[Oroboros O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]]
+|keywords=Tissue homogenate, Shredder
+|mipnetlab=AT_Innsbruck_Oroboros, AT Innsbruck MitoCom
 }}
 {{Labeling
-|instruments=Oxygraph-2k, O2k-Manual, Protocol, O2k-Manuals & Protocols, MiPNet-Publication
+|area=Respiration, Instruments;methods, Comparative MiP;environmental MiP
+|organism=Fishes
+|tissues=Heart, Liver
 |preparations=Homogenate
-|additional=mt-Preparation
+|couplingstates=LEAK, OXPHOS, ET
-|articletype=Protocol; Manual, MiPNet-online Publication
+|pathways=N, S, NS
+|instruments=Oxygraph-2k, O2k-Protocol
+|additional=O2k-Demo, Mt-Preparation, Auxiliary, PBI-Shredder, O2k-Core
 }}
+== Further information ==
-== References ==
+:» [[PBI-Shredder_O2k-Set | PBI-Shredder O2k-Set]]
-* [[Gross_2011_Analyt Biochem|Gross VS, Greenberg HK, Baranov SV, Carlson GM, Stavrovskaya IG, Lazarev AV, Kristal BS (2011) Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization. Analyt Biochem 418: 213-223]].
-* Doerrier C, Draxl A, Wiethüchter A, Eigentler A, Gnaiger E (2012) Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. An application study with the PBI-Shredder. Mitocondr Physol Network 17.03: 1-12.
-* Pressure BioSciences Inc. The Shredder SG3 and Shredder PULSE Tubes: Product Specification Sheet: 1-2.
-* Pressure BioSciences Inc. The Shredder SG3: User Manual: 1-16.
-''Mitochondr Physiol Network – MiPNet Manuals and Protocols''
-== Author contributions and progress ==
-Prepared by Doerrier Velasco C, Draxl A, Eigentler A and Gnaiger E. DC, Wiethüchter A and DA performed the experiments.
-* First on-line version: 2012-05-14.
-[[Category:OroboroPedia]]
+[[Category:OroboPedia]]