MiPNet12.23 FiberRespiration: Difference between revisions
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|additional=O2k-Demo, O2k-Core, SUIT-011 | |additional=O2k-Demo, O2k-Core, SUIT-011, SUIT-011 O2 pfi D024, | ||
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== SUIT protocol == | == SUIT protocol == |
Revision as of 11:18, 14 February 2019
Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. |
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Oroboros (2019-01-10) Mitochondr Physiol Network
Abstract: Lemieux H, Votion DM, Doerrier C, Gnaiger E (2019) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23(08):1-4. Methodological and conceptual features of High-Resolution Respirometry (HRR) are illustrated in an experiment with permeabilized fibres in the Oroboros O2k. The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab software is shown for real-time data analysis (compare MiPNet12.09). The following guideline describes the SUIT-011 protocol and includes a short discussion of results (see Votion 2012 PLoS One). The experiment was carried out by participants of an O2k-Workshop on HRR in December 2007 (IOC44; Schroecken, Austria).
- Β» Product: Oroboros O2k, O2k-Catalogue
β’ O2k-Network Lab: AT_Innsbruck_Oroboros, BE_Liege_Votion DM
Labels: MiParea: Respiration, Instruments;methods
Organism: Horse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: Coupling efficiency;uncoupling, Cyt c Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Protocol
O2k-Demo, O2k-Core, SUIT-011, SUIT-011 O2 pfi D024
SUIT protocol
- SUIT protocol: SUIT-011
SUIT states: 1-2GM 3-4GMS 5S 6ROX
Step | Respiratory state | Pathway control | ET-Complex entry into Q-junction |
---|---|---|---|
1GM | GML | N | CI |
2D | GMP | N | CI |
2c | GMP | N | CI |
3S | GMSP | NS | CI&II |
4U | GMSE | NS | CI&II |
5Rot | SE | S | CII |
6Ama | ROX |
Figure 1. Oxygen concentration ([ΞΌM] blue line) and oxygen flux per mg wet weight of muscle ([pmolβs-1βmg-1] red lines) in O2k chamber B, in permeabilized fibres from horse skeletal muscle with the standard titration protocol.
- A substrate-uncoupler-inhibitor titration (SUIT) protocol (Fig. 1) was developed for respiratory studies of permeabilized muscle fibres. The protocol aims at
- an assessment of physiologically relevant maxumum mitochondrial respiratory capacity,
- additivity at the Q-junction
- coupling/pathway control.
- A substrate-uncoupler-inhibitor titration (SUIT) protocol (Fig. 1) was developed for respiratory studies of permeabilized muscle fibres. The protocol aims at
Reference
- Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. - Β»Bioblast linkΒ«