Difference between revisions of "MiPNet12.17 BovineHeartMito"
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|info=[[Media:MiPNet12.17 FrozenBovineHeartMito.pdf|'''Open Access-MiPNet12.17.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet12.17_FrozenBovineHeartMito.pdf Versions] | |info=[[Media:MiPNet12.17 FrozenBovineHeartMito.pdf|'''Open Access-MiPNet12.17.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet12.17_FrozenBovineHeartMito.pdf Versions] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year= | |year=2011-12 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Petrosyan S, Naviaux R, Haas RH, Gnaiger E ( | |abstract='''Petrosyan S, Naviaux R, Haas RH, Gnaiger E (2011) Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. Mitochondr Physiol Network 12.17(04).''' | ||
The following demo experiment was performed in preparation for the lecture hall experiment, presented at the Mitochondrial Medicine conference (Diagnosis of Disease: Workshop on HRR at Mitochondrial Medicine 2007, UMDF, San Diego, USA, June 2007 (IOC40). For explanation of symbols, see [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]. | The following demo experiment was performed in preparation for the lecture hall experiment, presented at the Mitochondrial Medicine conference (Diagnosis of Disease: Workshop on HRR at Mitochondrial Medicine 2007, UMDF, San Diego, USA, June 2007 (IOC40). For explanation of symbols, see [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]. | ||
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[[Image:MiPNet12.17.jpg|center|100px|frame|Figure 1. Simultaneous display of oxygen concentration (blue lines) and oxygen flux (respiratory rate, red lines; negative time derivative of oxygen concentration) in chamber A and B.]] | [[Image:MiPNet12.17.jpg|center|100px|frame|Figure 1. Simultaneous display of oxygen concentration (blue lines) and oxygen flux (respiratory rate, red lines; negative time derivative of oxygen concentration) in chamber A and B.]] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS}} | |mipnetlab=AT_Innsbruck_OROBOROS | ||
}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods | |area=Respiration, Instruments;methods | ||
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|substratestates=CII | |substratestates=CII | ||
|instruments=Oxygraph-2k, Protocol | |instruments=Oxygraph-2k, Protocol | ||
|additional=O2k-Demo, O2k-Core}} | |additional=O2k-Demo, O2k-Core | ||
}} | |||
== Further information == | == Further information == | ||
:'''>> [http://www.oroboros.at/index.php?id=protocols_mitos_heart Excel Templates and DatLab-Demo Files]''' | :'''>> [http://www.oroboros.at/index.php?id=protocols_mitos_heart Excel Templates and DatLab-Demo Files]''' |
Revision as of 19:21, 14 June 2014
Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. |
Β» Open Access-MiPNet12.17.pdf; Versions
OROBOROS (2011-12) Mitochondr Physiol Network
Abstract: Petrosyan S, Naviaux R, Haas RH, Gnaiger E (2011) Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. Mitochondr Physiol Network 12.17(04).
The following demo experiment was performed in preparation for the lecture hall experiment, presented at the Mitochondrial Medicine conference (Diagnosis of Disease: Workshop on HRR at Mitochondrial Medicine 2007, UMDF, San Diego, USA, June 2007 (IOC40). For explanation of symbols, see Gnaiger 2012 MitoPathways.
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Bovines
Tissue;cell: Heart
Preparation: SMP
Regulation: Coupling efficiency;uncoupling, Cyt c Coupling state: OXPHOS
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-Core