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MiPNet12.15 RespiratoryStates

From Bioblast
Publications in the MiPMap
Gnaiger E (2007-2011) MitoPathways: Respiratory states and flux control ratios. Mitochondr Physiol Network 12.15. - New edition: Gnaiger 2014.

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Abstract: In oxidative phosphorylation, the endergonic process of phosphorylation of ADP to ATP is coupled to the exergonic process of electron transfer to oxygen. Coupling is achieved through the proton pumps generating and utilizing the protonmotive force in a proton circuit across the inner mitochondrial membrane. This proton circuit is partially uncoupled by proton leaks. Three different meanings of uncoupling (or coupling) are distinguished by defining intrinsically uncoupled, pathologically dyscoupled, and experimentally non-coupled respiration.

Respiratory steady states have been clearly defined by Chance and Williams (1955) according to a protocol for oxygraphic experiments with isolated mitochondria. The present state of terminology, however (e.g. State 2, requires clarification, particularly for extending bioenergetics to mitochondrial respiratory physiology of the living cell. β€’ Keywords: Archive

β€’ O2k-Network Lab: AT_Innsbruck_Oroboros


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MitoPathways 


Further information

>>MiPNet12.15

In: Mitochondrial Pathways and Respiratory Control. Oroboros MiPNet Publ.

Abbreviations

A1.1. Abbreviations for substrates

of the TCA cycle and major entries (single capital letters for the most commonly used substrates)

A1.2. Other substrates and redox components of the respiratory system

A1.3. Phosphorylation system

(adenylates, Pi, uncouplers, downstream inhibitors of ATP synthase, ANT, or phosphate) are denoted by subscripts. If Pi is always present at saturating concentration, it does not have to be indicated in the titration protocols.

  • Pi Inorganic phosphate
  • N no adenylates added (state LN)
  • D ADP at saturating concentration (state P: saturating [ADP])
  • D0.2 ADP at specified concentration (saturating versus non-saturating ADP is frequently not specified in State 3)
  • T ATP (state LT)
  • TD ATP+ADP (state P, in the presence of physiological high (mM) ATP concentrations)
  • T[ADP] High ATP and varying ADP concentrations, in the range between states T and TD.
  • 0my Oligomycin (state LOmy)
  • Atr Atractyloside (state LAtr)
  • u Uncoupler at optimum concentration for maximum non-coupled flux (state E).

A1.4. Inhibitors

of respiratory complexes, dehydrogenases or transorters:

A1.5. Respiratory states and flux control ratios

 Coupling control states
 Coupling control ratios (CCR)

References