Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MiPNet08.18 LactateDehydrogenase"

From Bioblast
 
Line 1: Line 1:
{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]]Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme.  
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]]Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme.  
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/4/47/MiPNet08.18_LDH.pdf |Bioblast pdf]]  
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/4/47/MiPNet08.18_LDH.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet08.18_LDH.pdf Versions]  
|authors=Oroboros
|authors=Oroboros
|year=2010-08-06
|year=2010-08-06
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18.''' »[http://www.bioblast.at/index.php/File:MiPNet08.18_LDH.pdf Versions]
|abstract='''Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18.'''  


Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test.
Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test.

Latest revision as of 10:03, 11 January 2018

Publications in the MiPMap
O2k-Protocols contents
Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme.

» Bioblast pdf »Versions

Oroboros (2010-08-06) Mitochondr Physiol Network

Abstract: Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18.

Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test.

>> Product: Oroboros O2k, Oroboros O2k-Catalogue

Keywords: Enzymes

O2k-Network Lab: AT_Innsbruck_Oroboros


Labels: MiParea: Instruments;methods 



Preparation: Enzyme  Enzyme: Marker enzyme 


HRR: O2k-Protocol 

Mt- and marker-enzymes