Difference between revisions of "Layout for DatLab graphs"

Revision as of 15:58, 17 May 2016

high-resolution terminology - matching measurements at high-resolution

Layout for DatLab graphs

Description

A Layout in DatLab is a predefined selection of Plots to be displayed at specific Scalings and in a specific order. Any defined graphics Layout can be selected from the Layout menu for defining initial settings for the Plots [Ctrl+F6] and Scaling [F6]. A Layout can be modified and saved under a specific name. In typical standard DatLab graph layouts plots are shown for the left chamber in Graph 1 (top), and for the right chamber in Graph 2 below). During data acquisition, a 30 min time range is frequently used and adjusted to 1 h during the experiment [F6].

Reference: MiPNet19.18C DatLab Guide

MitoPedia O2k and high-resolution respirometry: DatLab

Layout: O2k-Core

Version: DatLab 7

1 Calibration show temp

2a Calibration and background

2b Calibration and background (high O2)

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3a Flux per volume

3b Flux per volume (high O2)

3c Flux per volume overlap

3d Flux per volume overlap (high O2)

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4a Specific flux

4b Specific flux (high O2)

4c Specific flux overlap

4d Specific flux overlap (high O2)

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5a Flux control ratio

5b Flux control ratio (high O2)

5c Flux control ratio overlap

5d Flux control ratio overlap (high O2)

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Raw signal

1 Calibration show temp

General information

Layouts 1 ff. are considered for O2k-Core applications.

Layouts Amp1 ff. or pX1 ff. are selected for O2k-MultiSensor applications.

O2 calibration experiment with temperature and Peltier power in Graph 3: This is the default layout used after switching on the O2k.

Graph 1 & 2: O2 concentration (blue lines, left Y-axis) and O2 slope neg. (uncorrected; red lines, right Y-axis) are displayed, in the top graph for the left chamber (A), and below for the right chamber (B).

Graph 3: The third graph (bottom) shows the block temperature on the left Y axis (red) and the Peltier power on the right Y axis (green). Only when both temperature and Peltier power are constant, the chambers have reached thermal equilibrium.

During this phase a stirrer test can be performed [F9].
The next step is to observe equilibration of the oxygen signal with a defined gas phase above the stirred aqueous phase ('open') chamber; usually with air as the first step to perform an oxygen sensor calibration.

» O2k-Manual: MiPNet19.18A_O2k-Start

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 == Layout: O2k-Core ==
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 :::: ----
 :::: Raw signal
+=== 1 Calibration show temp ===
+::: '''General information'''
+:::: Layouts 1 ff. are considered for O2k-Core applications.
+:::: Layouts Amp1 ff. or pX1 ff. are selected for O2k-MultiSensor applications.
+::: '''O2 calibration experiment''' with temperature and Peltier power in Graph 3: This is the default layout used after switching on the O2k.
+:::: Graph 1 & 2: O2 concentration (blue lines, left Y-axis) and O2 slope neg. (uncorrected; red lines, right Y-axis) are displayed, in the top graph for the left chamber (A), and below for the right chamber (B).
+:::: Graph 3: The third graph (bottom) shows the block temperature on the left Y axis (red) and the Peltier power on the right Y axis (green).  Only when both temperature and Peltier power are constant, the chambers have reached thermal equilibrium.
+::::# During this phase a stirrer test can be performed [F9].
+::::# The next step is to observe equilibration of the oxygen signal with a defined gas phase above the stirred aqueous phase ('open') chamber; usually with air as the first step to perform an oxygen sensor calibration.
+::::» O2k-Manual:   [[MiPNet19.18A_O2k-Start]]