Difference between revisions of "Klingenberg 1970 Eur J Biochem"
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{{Publication | {{Publication | ||
|title=Klingenberg M (1970) Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13: 247- | |title=Klingenberg M (1970) Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13:247-52. | ||
|info=PMID: 5439930 | |info=[http://www.ncbi.nlm.nih.gov/pubmed/5439930 PMID: 5439930] | ||
|authors=Klingenberg M | |authors=Klingenberg M | ||
|year=1970 | |year=1970 | ||
|journal=Eur J Biochem | |journal=Eur J Biochem | ||
|abstract=Evidence for the localization of the mitochondrial glycerol-phosphate dehydrogenase in the outer phase of the inner membrane is presented. | |abstract=Evidence for the localization of the [[Glycerophosphate dehydrogenase complex |mitochondrial glycerol-phosphate dehydrogenase]] in the outer phase of the inner membrane is presented. | ||
# Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate. | # Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate. | ||
# The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers. | # The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers. | ||
| Line 13: | Line 13: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |area=Respiration | ||
| | |preparations=Isolated mitochondria | ||
|enzymes=Complex II; | |enzymes=Complex II;succinate dehydrogenase | ||
|topics=Uncoupler | |||
|couplingstates=ET | |||
|pathways=Gp | |||
|additional=Glycerol-phosphate dehydrogenase, Uncoupler | |additional=Glycerol-phosphate dehydrogenase, Uncoupler | ||
}} | }} | ||
Latest revision as of 14:55, 13 November 2017
| Klingenberg M (1970) Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13:247-52. |
Klingenberg M (1970) Eur J Biochem
Abstract: Evidence for the localization of the mitochondrial glycerol-phosphate dehydrogenase in the outer phase of the inner membrane is presented.
- Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate.
- The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers.
- In mitochondria from various tissues ferricyanide can interact directly with glycerolphosphate dehydrogenase but not with succinate dehydrogenase.
- The oxidation of glycerol-phosphate, in contrast to that of succinate, is insensitive to substrate depletion brought about by uncouplers.
- The results are interpreted to show that both the substrate and the acceptor-site of glycerol-phosphate dehydrogenase are exposed to the outer side of the inner membrane. Thus, for the operation of the glycerol-phosphate dehydrogenase, mitochondria do not require translocases for glycerol-phosphate and dihydroxyacetone-phosphate.
Labels: MiParea: Respiration
Preparation: Isolated mitochondria
Enzyme: Complex II;succinate dehydrogenase
Regulation: Uncoupler
Coupling state: ET
Pathway: Gp
Glycerol-phosphate dehydrogenase, Uncoupler
