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K-Regio MitoFit

From Bioblast


MitoFit Preprints         MitoFit Preprints        
Gnaiger 2019 MitoFit Preprints
       
Gnaiger MitoFit Preprints 2020.4
        MitoFit DOI Data Center         MitoPedia: Preprints         Bioenergetics Communications


K-Regio MitoFit

The project MitoFit highlights the benefits of mitochondrial fitness.


* English - »German

MitoFit

K-Regio project MitoFit

The K-Regio project MitoFit aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. MitoFit will provide a signature to stimulate health tourism in the Alpine setting, introducing a scientific perspective on the benefits of mitochondrial fitness ranging from outdoor sports to quality of life and healthy ageing supported by physiotherapy.

MitoFit in health and preventive medicine: Physical inactivity is one of the greatest drivers of metabolic dysregulation and preventable diseases in modern societies, and has an impact on ageing populations in particular. It is becoming increasingly apparent that high and robust mitochondrial capacities are central for sustained metabolic health throughout life. Active lifestyles increase mitochondrial capacities and reduce the risk of degenerative diseases. Despite the clear role of high and efficient mitochondrial capacities in preventing metabolic diseases, promoting health and improving life quality in ageing populations, there is currently no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals, populations, or among populations. The O2k is the only instrument with sufficient stability and reliability for quantitative high-resolution respirometry (HRR). This world-leading technology offers the unique potential for developing the quality control tools required to establish the O2k as a calibrated and standardised in vitro diagnostic device. Overall, we aim to gain a CE Mark and FDA approval for the O2k, following the innovations on the level of a quality control system, and to transform this high-resolution research instrument into an essential medical device to monitor metabolic health – maintaining mitochondria fit and well to live a healthier, longer life.

The project MitoFit is funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Applicant: Medical University of Innsbruck, A.Univ.-Prof. Dr. Erich Gnaiger
Application: 2015-04-24; Granted: 2015-09-16
Project duration: 2015-05-01 to 2018-04-30
Total budget: € 1.26 Mill; financial support by Tyrolian Government: € 0.89 Mill.


» MitoFit Training Camp Kuehtai 2016


Concept

MitoFit will provide a unique opportunity within fields of recreation, sports, wellness, lifestyle monitoring and preventive medicine programs. Collaborations with established regional initiatives will leverage on current research initiatives and established expertise in the measurement of mitochondrial function and competence. This will translate to tangible outcomes for the MitoFit concept as a marketable health, sports performance and medical diagnostic device. The establishment of the MitoFit-Knowledge Management Platform with a database reposited in the MitoFit Centre of Excellence (Fig. 1) will also provide a keystone to support the leadership of the mitochondrial monitoring instruments developed in translational research on mitochondrial competence (e.g. previous K-Regio project MitoCom) in close collaboration with local industrial partners.

The O2k-Core and O2k-Fluorometer, which are the gold standard for generating reliable quantitative respirometric data, will be the instruments of choice to build the MitoFit database. Standardized protocols developed by the MitoFit project will be streamlined for applications of the O2k-MitoFit. This will lead to a significant expansion of the world-wide market for the O2k, and drive the transition of the O2k from a well established specialized research tool to a standard health and biomedical instrument.

Figure 1: Structure of the MitoFit project. The development of the MitoFit- Knowledge Management Platform as an integrated module of the O2k technology system is in the centre of the project is. Strategies will be developed to pave the way towards obtaining a CE Mark (EU) and gaining regulatory approval by the Food and Drug Administration (FDA, US). Strategy development for hardware and software will be complemented by innovations in laboratory standards. A quality control system unique in the field of mitochondrial respirometry will be developed, from standardized protocols and sample preparation to the world-wide innovation of a proficiency ring test. These developments will be tested and applied in work packages introducing the MitoFit approach into the fields of lifestyle analyses based on diagnostic assays of mitochondrial function in blood cells. Health, sport and training programmes will be developed to improve mitochondrial fitness, with a focus on protective medicine, well-being and healthy ageing. The experience gained in implementing laboratory quality controls and during application developments will provide feedback into the structure and function of the MitoFit-Knowledge Management Platform.


Objectives: The objective is to achieve the transformation for the O2k from a research instrument to a medical device. The O2k is a high-end product for measuring mitochondrial function by high-resolution respirometry and is currently distributed in 44 countries world-wide. In an increasing number of cases, we are faced with demands on CE, FDA or equivalent approval for clinical diagnosis. Presently, there is no other instrument available on the market which meets such requirements. While continuing our strategy on Open innovation to maintain our technological leadership in mitochondrial respiratory research, the objective of the present project is to initiate an additional dimension of Strategic innovation towards standardization and comprehensive quality control as required for a medical device.


Technological and scientific innovation

The MitoFit-Knowledge Management Platform will be developed as an integral component of the O2k-technology. It will provide the first standardized measures to link human mitochondrial health to physiome data (heart rate, BMI, blood pressure, blood chemistry, VO2max). The technological ‘sole source’ status will be systematically secured. An increasing amount of respirometric data on mitochondrial physiology is generated world-wide, enlarging the theoretically available data base at an increasing speed. However, due to lacking standards as to how results are reported, most published data are not available for quantitative meta-analysis.
Even within research groups, data from different projects have to be tediously re-analyzed for quantitative comparison. By extending O2k applications to monitoring personal mitochondrial fitness and diagnosing an increasing range of health conditions, the development of a knowledge management platform will become even more important and mandatory for considerations on CE and FDA approval. The design of the MitoFit-Data Management System will provide the conceptual framework for strategic innovations related to a quality control system for standardization of instrumental quality tests and mitochondrial reference protocols, establishment of reference samples for proficiency tests, and guidelines for data analysis and presentation. Based on a collection of data obtained through this platform, users will be able to search from pre-defined fields or through full-text search for data, documents and a variety of areas of interest. In combination with web-based collaboration tools implemented in the software, the platform will both broaden the actually available data base for all researchers and stimulate the development of research networks.
Reference sample of cryopreserved mitochondria: The development of a reference sample for respirometry will provide enormous benefits for scientific research and open up new perspectives on clinical applications, enabling a presently unattainable level of quality control in respiratory studies which will (1) increase the scientific potential of on-going and newly initiated mitochondrial studies in basic and applied research. (2) The reference sample will provide the possibility for quality control of quantitative data for direct comparison and evaluation of functional mitochondrial diagnosis on patients monitored over the timecourse of their disease and treatment, and diagnosed by different clinical laboratories. (3) A reference sample can contribute significantly to the substitution and reduction of experiments with sacrificed animals, by (a) replacing animal tissue used for training and routine quality control, or (b) for methodological development, and (c) reducing the number of artefacts in experiments with animal or human tissue by detecting methodological deficiencies in routine quality control using the reference sample.
Cryopreservation of human blood cells: Obtaining tissue biopsies for the study of mitochondrial function is an invasive approach that requires a clinician to obtain the samples under strictly sterile conditions and that usually goes along with the need to immediately use the samples for measurements. The possibility to measure mitochondrial function in human blood cells will help to overcome these limitations by allowing a far less invasive sampling procedure applicable by anyone proficient in blood drawing and by enabling the collection and storage of samples for later measurements and analysis. It will also allow the repeated sampling of the same individual, as the burden of sampling is dramatically reduced. Altogether, the advantages of using cryopreserved human blood cells for respirometry will immensely widen the applicability of respirometry for the study of human physiology and will provide a significant stimulus for the entire research field.
MitoFit proficiency test: A proficiency ring test, probing the performance of participating laboratories, will be applied using the reference samples developed in a previous step. Measuring the respiration of reference samples at two pre-defined time points and following strictly defined experimental protocols, it will be possible for the first time to evaluate and compare the proficiency of different labs and to assess the reproducibility of measurements conducted, thereby having a measure of quality control at hand. Based on the outcome of the test, individual labs may set adequate steps to improve their performance and to develop lab SOPs supporting compliance with defined standard requirements.

Current state-of-the-art:

Figure 2: Substrate-uncoupler-inhibitor titration (SUIT) protocols.
A: Combined determination of oxygen consumption and H2O2 flux by O2k-Fluorometry in permeabilized HEK cells. Substrate and coupling states are shown in the upper and lower bars. Substrate states are limited to CI- and CI&II-linked respiration. From Makrecka- Kuka et al (2015).
B: Coupling/substrate control diagrams for experimental protocol design, including multiple substrate states for fatty acid oxidation, FAO (octanoylcarnitine & malate, OctM), CI-linked (addition of glutamate, G), CI&II-linked (addition of succinate, S), and CII-linked (addition of rotenone, Rot) respiration. Flux control ratios are normalized relative to ETS capacity with convergent CI&II (&FAO) electron input. Arrows 1, 2 and 3 indicate different SUIT protocols with partially overlapping respiratory states. From Gnaiger (2014).

Knowledge Management Platform: Many research groups are working on extensive documentation of genetic, epigenetic, transcriptomic, proteomic, lipidomic and metabolomic datasets in basic and clinical research, including mitochondrial medicine. Relevant techniques include next-generation sequencing to determine the genome (nDNA and mtDNA), methylome, and transcriptome (RNA), and mass spectrometry (proteome, lipidome, K-Regio: MitoFit 4 MitoFit www.mitofit.org metabolome). Such data are available online through various portals, some of which provide Open Access to allow researchers to use the data for addressing their specific interests and working hypotheses.

The capacity and efficiency of energy transformation through the mitochondrial pathways of core energy metabolism represent key features of the mitochondrial phenotype, but no corresponding data base is available for mapping mitochondrial health versus disease in humans. Performance parameters of OXPHOS are not available in a consistent format for cataloguing and, therefore, are not represented in presently available databases. Quantitative comparability of data generated by different labs on OXPHOS performance is restricted due to different protocols applied for preparing experimental solutions (solution protocols) and lack of standardization of procedure protocols including instrumental performance protocols, mitochondrial and cell preparation protocols and experimental titration protocols (SUIT protocols; Fig. 2).

With more than 280 and 150 O2k instruments sold in the EU and the US, respectively, it appears that we are not required to obtain a CE Mark or FDA clearance or approval for the O2k. However, this status will change when extending the applications from a research instrument to a medical device intended to report personal mitochondrial fitness disposition results for a wide range of health conditions, including the propensity for degenerative and other complex diseases. According to our knowledge of the available literature, the OROBOROS O2k is the only instrument on the world-wide market providing system accuracy criteria compatible with implementation of a Quality Control System for use as an In Vitro Diagnostic (IVD) device.

Cryopreservation: The current lack of a reference sample offering sufficient stability of respiratory parameters (intact mitochondrial membranes, preserved mitochondrial respiratory capacity, coupled oxidative phosphorylation) during storage has so far prevented implementation of proficiency testing. Application of stabilizers such as dimethylsulfoxide and polyethylene glycol yields controversial results on preserving mitochondrial function (Tab. 1). Mitochondrial respiratory competence has been reported in mononuclear human blood cells directly after storage at -80°C (Karabatsiakis et al 2014). Since cultured cells are routinely stored at -80 °C and cultured after thawing, it appears most promising to optimize conditions for cryopreservation of respiratory functions in a selected mammalian cell culture.
Assessing fitness and performance in humans: Standardized protocols are available to assess physical fitness by performance testing of athletes and patients, e.g. with spiroergometry for monitoring the success of training or therapeutic interventions (WP 7). In addition, biomarkers in blood samples are commonly monitored for risk evaluation (glucose tolerance test, HbA1c, ß-cell function and insulin resistance, triglycerids, cholesterol – TC, HDL, LDL), inflammatory processes (C-reactive protein, IL-6 and TNF-alpha) and disturbances of oxidative balance (derivatives of reactive oxygen metabolites; biological antioxidant potential test). Available blood tests providing an index of mitochondrial function are limited by over-simplified protocols, low resolution and controversial interpretation (Chacko et al 2014). Respirometric SUIT protocols for OXPHOS analysis (Sjoevall et al 2010, 2013, Pecina et al 2014) need to be extended by combined measurement of hydrogen peroxide production to provide additional information on mitochondrial-specific oxidative stress (De Lucas et al 2014).
Diagnostic evaluation of cryopreserved samples: Karabatsiakis et al (2014) collected blood samples from patients diagnosed for major depression, separated peripheral blood mononuclear cells and froze these cells in a standard culture freezing medium for cryopreservation at -80 °C. Cells were subsequently thawed and examined by HRR. ROUTINE respiration, electron transfer system capacity and coupling efficiency were diminished, in direct correlation with the severety of major depression. With this group as an International Strategic Partner, this approach will be extended for application to life style diagnostics in preventive medicine.

Table 1 MitoFit Table 1.jpg


Partners

Medical University Innsbruck MUI: UniversitĂ€tsklinik fĂŒr Visceral-, Transplantations- und Thoraxchirurgie, D. Swarovski Forschungslabor, DSL (Ao. Univ.-Prof. Dr. Erich Gnaiger)

https://www.i-med.ac.at/patienten/ukl_transplant-chirurgie.html

Medical University Innsbruck MUI: Biozentrum Innsbruck - Sektion fĂŒr Bioinformatik, SBI (Univ.-Prof. Dr. Zlatko Trajanoski)

http://icbi.i-med.ac.at/

LF-University Innsbruck LFU: Institut fĂŒr Sportwissenschaften, ISW (Univ.-Prof. DDr. Martin Burtscher)

http://www.uibk.ac.at/isw/

OROBOROS SME: OROBOROS INSTRUMENTS GmbH, Innsbruck, ORO (Dr. Erich Gnaiger, Mag.a. Verena Laner)

http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS

WGT SME: WGT-Elektronik GmbH & Co KG, Kolsass, Tyrol, WGT (Philipp Gradl)

http://www.wgt.at

HLZ Kuehtai SME: Höhenleistungszentrum Betriebsg.m.b.H., Innsbruck, HLZ (GeschĂ€ftsfĂŒhrer Mag. Andreas Brix, Robert Valentini)

http://www.hoehentraining-kuehtai.at/

Sporttherapie Huber SME: Sporttherapie Mag. Huber GmbH, Innsbruck, STH (Mag. Reinhard Huber)

http://www.sporttherapie-huber.at

MUI = Medical University Innsbruck; LFU = Leopold-Franzens-University Innsbruck; SME = Small and medium-sized enterprise

External consultants

  • MUI: Department fĂŒr Medizinische Statistik, Informatik und Gesundheitsökonomie, MSIG (Univ.-Doz. Dr. Georg Göbel)
https://www.i-med.ac.at/msig/
  • software security networks - ssn (Lukas Gradl)
http://www.ssn.at/
  • fancy tree films (Johannes AitzetmĂŒller)
http://www.fancytreefilms.com/
  • en.co.tec Schmid KG, Technisches BĂŒro fĂŒr Elektrotechnik (Dipl.- Ing. Martin Schmid)
http://www.encotec.at/

International strategic partners - Europe

ES Barcelona Garcia-Roves PM
DE Ulm Karabatsiakis A
SK Bratislava Sumbalova Z

International strategic partners - overseas

  • Goodpaster Bret H, Prof. Dr: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Sanford/Burnham Institute, Orlando, FL, US
US FL Orlando Goodpaster BH
NZ Auckland Hickey AJ
  • Molina Anthony JA, PhD: J. Paul Sticht Center on Aging, Dept Internal Medicine, Wake Forest School of Medicine, Winston-Salem, NC, US
US NC Winston-Salem Molina AJA
US NC Greenville Neufer PD
ZA Cape Town Ojuka EO


Becoming ‘MitoFit’ – a trend towards a new lifestyle?

Lemawork Ketema, winner of the “Wings for Life – World Run“ 2014 and 2015, at the Höhenleistungszentrum in KĂŒhtai, where he has been training for the Rio marathon. From left to right: Lemawork Ketema, Erich Gnaiger (OROBOROS INSTRUMENTS), Verena Laner (OROBOROS INSTRUMENTS) and Andreas Brix (Höhenleistungszentrum KĂŒhtai)

With its innovative approach, MitoFit arouses a new awareness of preventive medicine, to which a physically active and calorie-balanced lifestyle can contribute significantly. Of particular importance is “healthy aging”, a generally improved quality of life and a reduced risk of a multiplicity of degenerative diseases influenced by the mitochondria: e.g. obesity, diabetes II, various forms of cancer and dementias. Mitochondrial competence will be made a reality to a larger proportion of the population and the regional identity strengthened both amongst our own population and amongst tourists through the offer of exercise-orientated leisure packages in the Alpine landscape of Tyrol. Making our mitochondria fit – MitoFit and well – may strengthen the trend to a less sedentary, healthier and balanced lifestyle, supported by the growing evidence of the central role of mitochondrial function for an improved and prolonged quality of life.


MitoFit topics

Related projects

  • Tiroler Innovationsförderung: Â» NextGen-O2k (Feb 2015 - Jan 2017)
  • K-Regio MitoCom: Â» MitoCom (completed 2014)

Relevant building blocks








MitoFit Publications

   
MiPNet28.08 Oroboros O2k Series J manual2023-10-19
O2k-Manual
Oroboros O2k Series J manual
MiPNet28.07 NextGen-O2k Series XB manual2023-10-19
O2k-Manual
NextGen-O2k Series XB manual
MiPNet26.13 NextGen-O2k manual2022-07-25
O2k-Manual
NextGen-O2k manual
MiPNet26.12 NextGen-O2k: NADH-Module2022-05-17
O2k-Manual
NextGen-O2k: NADH-Module manual
MiPNet26.11 NextGen-O2k: PB-Module2021-10-29
O2k-Manual
NextGen-O2k: PB-Module manual
MiPNet24.12 NextGen-O2k: Q-Module2021-10-29
O2k-Manual
NextGen-O2k: Q-Module manual
MiPNet24.10 H2O2 flux analysis2021-10-22
O2k-Protocols
Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
MiPNet26.10 MgG data analysis2021-09-16
O2k-Protocols
Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4
MitoEAGLE muscle 12020Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. - Updated: 2020-03-08
BEC 2020.1 doi10.26124bec2020-0001.v12020Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
MitoEAGLE blood cells 12020Åsander Frostner Eleonor*, Aburel Oana M*, Avram Vlad F*, Calabria Elisa, Castelo Rueda Maria Paulina*, Chamkha Imen*, ČiĆŸmĂĄrovĂĄ Beata, Danila Maria-Daniela*, Doerrier Carolina*, Eckert Gunter P*, Ehinger Johannes K, Elmer Eskil*, Garcia-Souza Luiz F*, Gnaiger Erich*, Hoppel Florian*, Karabatsiakis Alexander*, Keppner Gloria, Kidere Dita*, Krako Jakovljević Nina, Labieniec-Watala Magdalena*, Lelcu Theia*, Micankova Petra, Michalak Slawomir*, Molina Anthony JA*, Pavlovic Kasja, Pichler Irene*, Piel Sarah, Rousar Tomas, Rybacka-Mossakowska Joanna, Schartner Melanie, Siewiera Karolina*, Silaidos Carmina*, Sjövall Fredrik*, Sobotka Ondrej*, Sumbalova Zuzana*, Swiniuch Daria, Vernerova Andrea*, Volani Chiara*, Vujacic-Mirski Ksenija*, Watala Cezary* (2020) Interlaboratory guide to mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets. - Updated: 2020-03-06 - *Confirmed
Gnaiger 2020 BEC MitoPathways2020Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
MiPNet24.11 mtMP calculation2019-08-05
O2k-Protocols
Mitochondrial membrane potential calculation
MiPNet20.13 Safranin mt-membranepotential2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
MiPNet20.14 AmplexRed H2O2-production2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
MiPNet21.06 SUIT RP2018-06-25
O2k-Protocols
SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MĂ©szĂĄros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3
Miller 2017 PLOS ONE2017Miller B, Hamilton K, Boushel R, Williamson K, Laner V, Gnaiger E, Davis M (2017) Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLOS ONE 12:e0174874.
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
Schoepf 2016 FEBS J2016Schöpf B, SchÀfer G, Weber A, Talasz H, Eder IE, Klocker H, Gnaiger E (2016) Oxidative phosphorylation and mitochondrial function differ between human prostate tissue and cultured cells. https://doi.org/10.1111/febs.13733
Harrison 2015 J Appl Physiol2015Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. https://doi.org/10.1152/japplphysiol.00146.2015
Gnaiger 2015 Scand J Med Sci Sports2015Gnaiger E, Boushel R, SĂžndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DĂ­ez-SĂĄnchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612
Boushel 2015 Scand J Med Sci Sports2015Boushel R, Gnaiger E, Larsen FJ, Helge JW, Gonzalez-Alonso J, Ara I, Munch-Andersen T, van Hall G, Sþndergaard H, Saltin B, Calbet JAL (2015) Maintained peak leg and pulmonary VO2 despite substantial reduction in muscle mitochondrial capacity. Scand J Med Sci Sports 25 (Suppl 4):135–43.

MitoFit Events

 WhenWhere
MiPNet28.12 IOC164 Schroecken AT2024-09-30
O2k-Workshops.png
Schroecken AT, 2024 Sep 30 - Oct 05. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC164.
MiPNet28.13 IOC165 Innsbruck AT2024-09-02
O2k-Workshops.png
Innsbruck AT, 2024 Sep 02-04. EBEC2024 Satellite Oroboros O2k-Workshop: Mito&Chlora High-Resolution Respirometry and PhotoBiology, IOC165.
MiPNet28.11 IOC163 Schroecken AT2024-06-17
O2k-Workshops.png
Schroecken AT, 2024 Jun 17-22. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC163.
MiPNet28.02 IOC162 Schroecken AT2023-10-02
O2k-Workshops.png
Schroecken AT, 2023 Oct 02-07. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC162.
MiPNet28.01 IOC160 Schroecken AT2023-06-19
O2k-Workshops.png
Schroecken AT, 2023 Jun 19-24. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC160.
MiPNet28.05 IOC158 Innsbruck AT2023-02-27
OROBOROS-solo.png
Innsbruck AT, 2023 Feb 27 - Mar 01. O2k-Coaching Days - Basic, IOC158.
MiPNet27.04 IOC155 Schroecken AT2022-10-03
O2k-Workshops.png
Schroecken AT, 2022 Oct 03-08. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC155.
MiPNet25.02 IOC Schroecken AT2020
O2k-Workshops.png
Schroecken AT, Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC. - Due to COVID-19 we change the strategy and provide the O2k-Workshop in a virtual form.
MiPNet24.15 IOC143 Haryana IN2019-11-15
O2k-Network
Haryana IN, 2019 Nov 15. Oroboros O2k-Workshop on high-resolution respirometry: Assessment of mitochondrial function in health and disease IOC143.
MiPNet24.02 IOC141 Schroecken AT2019-09-23
NextGenO2k logo.jpg
Schroecken AT, 2019 Sep 23-28 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC141.
MiPNet24.01 IOC139 Schroecken AT2019-06-17
O2k-Workshops.png
Schroecken AT, 2019 June 17-22 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139.
DSL Retreat 2019 Innsbruck AT2019-02-21Vill AT, 2019 Feb 21. Third retreat of the Daniel Swarovski Research Laboratory (DSL).
MitoFit Conference 2019 Innsbruck AT2019-01-29
Logo MitoFit.jpg
Innsbruck AT, 2019 Jan 29. MitoFit Conference: Mitochondrial fitness in cancer metabolism, exercise and aging.
MiPNet23.11 IOC137 Innsbruck AT2018-12-10
OROBOROS-solo.png
Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC137.
PartneringDay 2018 Innsbruck AT2018-10-11Innsbruck AT, 2018 Oct 11. Partnering Day 2018
Fokus Tag: Targeting Phospholipids 2018 Heidelberg DE2018-10-05Heidelberg DE, 2018 Oct 05. Fokus Tag: Targeting Phospholipids.
MiPNet23.08 IOC134 Schroecken AT2018-10-01
OROBOROS-solo.png
Schroecken AT, 2018 Oct 01-06 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC.
Fokus Tag: Labordiagnostik Intensiv 2018 Heidelberg DE2018-09-16Heidelberg DE, 2018 Sep 16. Fokus Tag: Labordiagnostik Intensiv.
MiPNet23.04 IOC133 Innsbruck AT2018-09-05
OROBOROS-solo.png
Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC 133.
MiPNet23.06 IOC130 Schroecken AT2018-06-18
OROBOROS-solo.png
Schroecken AT, 2018 Jun 18-23 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC130.
Long Night of Research 2018 Innsbruck AT2018-04-13
LNF.JPG
Innsbruck AT, 2018 Apr 13. Oroboros at Long Night of Research, CCB. The diagnostic bioenergetic report – a milestone on the way to mitochondrial fitness and physical well-being.
Clinical Mitochondria- and Environmental Medicine 2018 Heidelberg DE2018-03-02Heidelberg DE, 2018 Mar 02-03. Clinical Mitochondria- and Environmental Medicine.
DSL retreat Innsbruck AT2018-02-15Vill AT, 2018 Feb 15. Second retreat of the Daniel Swarovski Research Laboratory (DSL).
MitoEAGLE Innsbruck 2018-01-292018-01-29
COST Action MitoEAGLE
Innsbruck AT, 2018 Jan 29 - Feb 01. WG 4 blood cells workshop and retreat - COST Action MitoEAGLE.
MiPNet23.01 IOC126 Innsbruck AT2018-01-24
OROBOROS-solo.png
Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126.
MitoFit Workshop ATP 2017 Innsbruck AT2017-12-18
MitoFit
Innsbruck AT, 2017 Dec 18-20. MitoFit Workshop on adenosine triphosphate (ATP).
MitoFit Open Seminar 2017-10-232017-10-23
MitoFit
Innsbruck AT, 2017 Oct 23. Daniel-Swarovski-Research Laboratory Research Seminar WS 2017/2018 - MitoFit Open Seminar on Respiration, cryopreservation and viability test in human blood cells: peripheral blood mononuclear cells (PBMC) and platelets.
MiPNet22.07 IOC124 Schroecken AT2017-10-03
OROBOROS-solo.png
Schroecken AT, 2017 Oct 03-08 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC124.
MitoEAGLE Obergurgl 20172017-07-27
COST Action MitoEAGLE
Obergurgl AT, 2017 Jul 27-30. 10th MitoEAGLE Workshop WG1-4.
MiPschool Obergurgl 20172017-07-23
COST Action MitoEAGLE
MiPsociety
Obergurgl AT, 2017 Jul 23-30. 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4.
MitoFit Open Seminar 2017-07-142017-07-14
MitoFit
Innsbruck AT, 2017 Jul 14. MitoFit Open Seminar on immune cell bioenergetics.
Investigating the role of mitochondria in healthy and psychopathological aging.
MiPNet22.01 IOC122 Schroecken AT2017-06-26
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Schroecken AT, 2017 Jun 26-Jul 01 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC122.
MiPNet22.06 IOC121 Cologne DE2017-06-12
Oroboros Instruments
Cologne DE, 2017 Jun 12. Oroboros O2k-Lunch Workshop on high-resolution respirometry (HRR), IOC121 at EUROMIT2017 Cologne DE.
Life Science PhD Meeting 2017 Innsbruck AT2017-04-18Innsbruck AT, 2017 Apr 18-19. Careers in Industry and PhD LifeScience Symposium.
Klinische Mitochondrienmedizin und Umweltmedizin 2017 Heidelberg DE2017-03-11Heidelberg DE, 2017 Mar 11-12. Klinische Mitochondrienmedizin und Umweltmedizin.
MiPNet22.03 IOC119 Innsbruck AT2017-02-27
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Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.
MiPNet21.19 IOC114 Innsbruck AT2016-12-12
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Innsbruck AT, 2016 Dec 12-15. MitoFit Coaching Days - Basic IOC114.
MiPNet21.11 IOC116 Innsbruck AT2016-11-21
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Innsbruck AT, 2016 Nov 21-25. Oroboros O2k-Workshop on O2k-Fluorometry - advanced, IOC116.
MiPNet21.10 IOC115 Schroecken AT2016-10-03
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Schroecken AT, 2016 Oct 03-08. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC115.
MiPNet21.18 IOC117 Kuala Lumpur MY2016-09-26
O2k-Network
Kuala Lumpur MY, 2016 Sep 26-30. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC117.
MiPNet21.09 IOC113 Havana CU2016-08-08
O2k-Network
Havana CU, 2016 Aug 08-09. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC113. O2k-Network Lab Â» CU Havana Pardo Andreu GL
MiPNet21.15 IOC112 Kuehtai AT2016-07-07
O2k-Network
Kuehtai AT, 2016 Jul 07-13. MitoFit Science Camp and IOC112.
MitoFit Science Camp 2016 Kuehtai AT2016-07-07
MitoFit
Kuehtai AT, 2016 Jul 07-13. MitoFit Science Camp and IOC112.
Long Night of Research 2016 Innsbruck AT2016-04-22
LNF.JPG
Innsbruck AT, 2016 Apr 22. OROBOROS at Long Night of Research, CCB. MitoFit – Training for the powerhouses of your blood- and muscle cells.
MiPNet21.01 IOC109 Schroecken AT2016-04-03
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Schroecken AT, 2016 Apr 03-08. OroborosO2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC109.
Expert/inn/en-Workshop Medizintechnik Innsbruck AT2016-02-29
MitoFit
Innsbruck AT, 2016 Feb 29. Expert/inn/en-Workshop Medizintechnik
MitoFit Workshop en.co.tec medical device 2016-02-042016-02-04
MitoFit
Innsbruck AT, 2016 Feb 04. MitoFit Workshop en.co.tec medical device.
MitoFit Workshop Blood Cells 2016-01-082016-01-08
MitoFit
Innsbruck AT, 2016 Jan 08. MitoFit Workshop on blood cell respirometry for mitochondrial research and diagnostic applications.
MiPNet20.10 IOC106 Schroecken2015-10-06
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Schroecken AT, 2015 Oct 06-11. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC106.
MiPNet20.16 IOC105 Riga2015-08-27
O2k-Network
Riga LV, 2015 Aug 27. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - innovabalt, IOC105.
MiPNet20.05 IOC104 Greenville2015-08-09
O2k-Network
Greenville NC US, 2015 Aug 09-10. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC104.
QualitÀtszirkel 20152015-06-30
MitoFit
Innsbruck AT, 2015 Jun 30. QualitÀtszirkel Programm pdf
Wissenschaft im Gespraech2015-06-10
MitoFit
Bregenz AT, 2015 Jun 10. Wissenschaft im GesprÀch: Erich Gnaiger, Zellforscher. Wissenschaft im GesprÀch