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Difference between revisions of "ISE-Ca2+ Membranes"

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{{Product
{{Technical support}}
|description=Membrane for Oroboros ISE Ca-electrode, PVC, 4 mm diameter, package of 5 membranes
__TOC__
|product id=32202-01
{{MitoPedia without banner
|product type=MultiSensor, Accessory, ISE, Consumables, Membranes
|abbr=
|info=[[Calcium electrode]], [http://www.oroboros.at/index.php=spares O2k-spares]
|description=[[Image:Ca2+ membranes.jpg|right|180px]]'''ISE-Ca2+ Membranes''': PVC, 4 mm diameter, box of 5 membranes.
To be used with the [[O2k-TPP+ ISE-Module]].
|info= [https://www.oroboros.at/index.php/product/ise-ca2-membranes/ '''Product details and purchase information''']
}}
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== Technical support ==
:::: The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al<ref>Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca<sup>2+</sup>-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.</ref>. The limit of detection is less than 10 nM in Ca<sup>2+</sup> buffered calibration solutions. The main difficulty in establishing reliable Ca<sup>2+</sup> measurements is not the Ca<sup>2+</sup> sensitive membrane but the methodology of its application in a medium simulating intracellular conditions.
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== Additional resources ==
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:::ยป [[MiPNet15.03 O2k-MultiSensor-ISE|ISE Manual]]
:::ยป [[O2k-TPP+ ISE-Module]]
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== References ==
<references/>
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{{MitoPedia O2k and high-resolution respirometry
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|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
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Latest revision as of 10:50, 11 November 2020


                  


O2k-Open Support

ISE-Ca2+ Membranes



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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

Ca2+ membranes.jpg

ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes.

To be used with the O2k-TPP+ ISE-Module.


Reference: Product details and purchase information


Technical support

The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al[1]. The limit of detection is less than 10 nM in Ca2+ buffered calibration solutions. The main difficulty in establishing reliable Ca2+ measurements is not the Ca2+ sensitive membrane but the methodology of its application in a medium simulating intracellular conditions.

Additional resources

ยป ISE Manual
ยป O2k-TPP+ ISE-Module

References

  1. โ†‘ Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca2+-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.


MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry