Difference between revisions of "ISE-Ca2+ Membranes"
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|description=[[Image:Ca2+ membranes.jpg|right|180px]]'''ISE-Ca2+ Membranes''': PVC, 4 mm diameter, box of 5 membranes. | |description=[[Image:Ca2+ membranes.jpg|right|180px]]'''ISE-Ca2+ Membranes''': PVC, 4 mm diameter, box of 5 membranes. | ||
To be used with the [[O2k-TPP+ ISE-Module]]. | To be used with the [[O2k-TPP+ ISE-Module]]. | ||
Revision as of 14:50, 2 November 2020
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ISE-Ca2+ Membranes |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes.
To be used with the O2k-TPP+ ISE-Module.
Reference: ISE Manual, O2k-TPP+ ISE-Module#Calcium_electrode, O2k-spares
Technical support
- The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al[1]. The limit of detection is less than 10 nM in Ca2+ buffered calibration solutions. The main difficulty in establishing reliable Ca2+ measurements is not the Ca2+ sensitive membrane but the methodology of its application in a medium simulating intracellular conditions. Further details: O2k-TPP+ ISE-Module#Calcium_electrode.
References
- ↑ Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca2+-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.
MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
