Difference between revisions of "ISE-Ca2+ Membranes"
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== Technical support == | == Technical support == | ||
:::: The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al<ref>Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca<sup>2+</sup>-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.</ref>. The limit of detection is less than 10 nM in Ca<sup>2+</sup> buffered calibration solutions. The main difficulty in establishing reliable Ca<sup>2+</sup> measurements is not the Ca<sup>2+</sup> sensitive membrane but the methodology of its application in a medium simulating intracellular conditions. Further details: [[O2k-TPP+ ISE-Module#Calcium_electrode]]. | :::: The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al<ref>Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca<sup>2+</sup>-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.</ref>. The limit of detection is less than 10 nM in Ca<sup>2+</sup> buffered calibration solutions. The main difficulty in establishing reliable Ca<sup>2+</sup> measurements is not the Ca<sup>2+</sup> sensitive membrane but the methodology of its application in a medium simulating intracellular conditions. Further details: [[O2k-TPP+ ISE-Module#Calcium_electrode]]. | ||
== References == | |||
<references/> | |||
Revision as of 14:50, 2 November 2020
ISE-Ca2+ Membranes
| Description |  To be used with the O2k-TPP+ ISE-Module. |
|---|---|
| Product ID | |
| Type | |
| Link | ISE Manual, O2k-TPP+ ISE-Module#Calcium_electrode, O2k-spares |
| Image |
Technical support
- The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al[1]. The limit of detection is less than 10 nM in Ca2+ buffered calibration solutions. The main difficulty in establishing reliable Ca2+ measurements is not the Ca2+ sensitive membrane but the methodology of its application in a medium simulating intracellular conditions. Further details: O2k-TPP+ ISE-Module#Calcium_electrode.
References
- ↑ Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca2+-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.
MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
