Goo 2013 Clin Exp Pharmacol Physiol: Difference between revisions
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Latest revision as of 11:40, 9 February 2022
Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Multiscale measurement of cardiac energetics. Clin Exp Pharmacol Physiol 40:671-81. |
Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Clin Exp Pharmacol Physiol
Abstract: Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies. โข Keywords: ATP flux, Cardiac enthalpy production, Cardiac heat production, Mitochondrial membrane potential, Mitochondrial proton leak, Mitochondrial reactive oxygen species production
โข O2k-Network Lab: NZ Auckland Hickey AJ
Cited by
- Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Heart Preparation: Permeabilized cells, Homogenate, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, NS
HRR: Oxygraph-2k
Labels, MgG, MitoFit 2021 MgG-PW