Gnaiger 2008 POS
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Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [pdf]
Contents
- Introduction
- Polarographic oxygen sensor and traditional oxygraphy
- High-resolution respirometry: the Oxygraph-2k
- Calibration of polarographic oxygen sensors and oxygen concentration in respiration media at air saturation
- From oxygraph slopes to respiratory flux corrected for background effects
- Phosphorylation control protocol with intact cells
- Titration steps of the PC protocol
- Experimental example for the PC protocol
- Flux control ratios from the PC protocol
- Intact cells, permeabilized cells and tissue, or isolated mitochondria
- Intact cells
- Permeabilized cells and tissue
- Isolated mitochondria
- Titration protocols in permeabilized cells, permeabilized tissue preparations, and isolated mitochondria
- Multisensor applications