Difference between revisions of "Gaviraghi 2021 Methods Mol Biol"
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|journal=Methods Mol Biol | |journal=Methods Mol Biol | ||
|abstract=Respirometry analysis is an effective technique to assess mitochondrial physiology. Insects are valuable biochemical models to understand metabolism and human diseases. Insect flight muscle and brain have been extensively used to explore mitochondrial function due to dissection feasibility and the low sample effort to allow oxygen consumption measurements. However, adequate plasma membrane permeabilization is required for substrates/modulators to reach mitochondria. Here, we describe a new method for study of mitochondrial physiology in insect tissues based on mechanical permeabilization as a fast and reliable method that do not require the use of detergents for chemical permeabilization of plasma membrane, while preserves mitochondrial integrity. | |abstract=Respirometry analysis is an effective technique to assess mitochondrial physiology. Insects are valuable biochemical models to understand metabolism and human diseases. Insect flight muscle and brain have been extensively used to explore mitochondrial function due to dissection feasibility and the low sample effort to allow oxygen consumption measurements. However, adequate plasma membrane permeabilization is required for substrates/modulators to reach mitochondria. Here, we describe a new method for study of mitochondrial physiology in insect tissues based on mechanical permeabilization as a fast and reliable method that do not require the use of detergents for chemical permeabilization of plasma membrane, while preserves mitochondrial integrity. | ||
|keywords=Animal models, Bioenergetics, Insect, Metabolism, Mitochondria, Respiration | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
Revision as of 16:17, 12 September 2022
| Gaviraghi A, Aveiro Y, Carvalho SS, Rosa RS, Oliveira MP, Oliveira MF (2021) Mechanical permeabilization as a New method for assessment of mitochondrial function in insect tissues. |
» Methods Mol Biol 2276:67-85. PMID: 34060033
Gaviraghi A, Aveiro Y, Carvalho SS, Rosa RS, Oliveira MP, Oliveira MF (2021) Methods Mol Biol
Abstract: Respirometry analysis is an effective technique to assess mitochondrial physiology. Insects are valuable biochemical models to understand metabolism and human diseases. Insect flight muscle and brain have been extensively used to explore mitochondrial function due to dissection feasibility and the low sample effort to allow oxygen consumption measurements. However, adequate plasma membrane permeabilization is required for substrates/modulators to reach mitochondria. Here, we describe a new method for study of mitochondrial physiology in insect tissues based on mechanical permeabilization as a fast and reliable method that do not require the use of detergents for chemical permeabilization of plasma membrane, while preserves mitochondrial integrity. • Keywords: Animal models, Bioenergetics, Insect, Metabolism, Mitochondria, Respiration • Bioblast editor: Plangger M
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