Extrinsic fluorophores: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=n.a. | |abbr=n.a. | ||
|description='''Extrinsic fluorophores''' are available for a wide range of parameters including ROS (H<sub>2</sub>O<sub>2</sub>, [[Amplex red]]) (HOO<sup>-</sup>, MitoSOX) , mitochondrial membrane potential ([[Safranin]], JC1, TMRM, Rhodamin 123), Ca<sup>2+</sup> (Fura2, Indo 1, Calcium Green) , pH (Fluorescein, HPTS, SNAFL-1), Mg<sup>2+</sup> (Magnesium orange) and redox state (roGFP). | |description='''Extrinsic fluorophores''' are molecules labelled with a fluorescent dye (as opposed to intrinsic fluorescence or autofluorescence of molecules which does not require such labelling). They are available for a wide range of parameters including ROS (H<sub>2</sub>O<sub>2</sub>, [[Amplex red]]) (HOO<sup>-</sup>, MitoSOX) , mitochondrial membrane potential ([[Safranin]], JC1, TMRM, Rhodamin 123), Ca<sup>2+</sup> (Fura2, Indo 1, Calcium Green) , pH (Fluorescein, HPTS, SNAFL-1), Mg<sup>2+</sup> (Magnesium orange) and redox state (roGFP). | ||
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{{MitoPedia methods | {{MitoPedia methods |
Revision as of 14:27, 30 August 2013
Description
Extrinsic fluorophores are molecules labelled with a fluorescent dye (as opposed to intrinsic fluorescence or autofluorescence of molecules which does not require such labelling). They are available for a wide range of parameters including ROS (H2O2, Amplex red) (HOO-, MitoSOX) , mitochondrial membrane potential (Safranin, JC1, TMRM, Rhodamin 123), Ca2+ (Fura2, Indo 1, Calcium Green) , pH (Fluorescein, HPTS, SNAFL-1), Mg2+ (Magnesium orange) and redox state (roGFP).
Abbreviation: n.a.
MitoPedia methods:
Fluorometry