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Excel templ: Protocols

From Bioblast

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Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.

This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.

Data for Excel template

Protocol name DLP# Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Step 14 Step 15 Step 16 Step 17 Step 18 Step 19 Step 20 # of steps State 1 State 2 State 3 State 4 State 5 State 6 State 7 State 8 State 9 State 10 State 11 State 12 State 13 State 14 State 15 State 16 State 17 State 18 State 19 State 20 Reference step Reference Mark Baseline step Baseline Mark Short name Notes Descriptions
SUIT-001_O2_mt_D001 D001 1mt 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 13 ROX N_L N_P N_P N_E N_E NS_E FNS_E S_E SGp_E ROX CIV ROX 7 5S 11 9Ama RP1_mt Doerrier 2018 Methods Mol Biol changed description
SUIT-001_O2_pfi_D002 D002 1pfi 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 13 ROX N_L N_P N_P N_E N_E NS_E FNS_E S_E SGp_E ROX CIV ROX 7 5S 11 9Ama RP1_pfi Doerrier 2018 Methods Mol Biol The SUIT-001 protocol in combination with SUIT-002 is specially designed to provide a common reference for comparison of respiratory control of mitochondrial preparations in a wide variety of species, tissues and cell types. SUIT-001 gives information of the linear coupling control (L- P- E) with NADH linked-substrates (PM). Moreover, the pathway control in ET state (N, NS, FNS, S and SGp pathways) can be evaluated by using this SUIT protocol. SUIT-001 can be extended with the CIV assay module.
SUIT-001_O2_ce-pce_D003 D003 ce1 1Dig 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 14 ce_R ROX N_L N_P N_P N_E N_E NS_E FNS_E S_E SGp_E ROX CIV ROX 8 5S 12 9Ama RP1_pce Doerrier 2018 Methods Mol Biol The SUIT-001 protocol in combination with SUIT-002 is specially designed to provide a common reference for comparison of respiratory control of mitochondrial preparations in a wide variety of species, tissues and cell types. SUIT-001 gives information of the linear coupling control (L- P- E) with NADH linked-substrates (PM). Moreover, the pathway control in ET state (N, NS, FNS, S and SGp pathways) can be evaluated by using this SUIT protocol. SUIT-001 can be extended with the CIV assay module.
SUIT-001_O2_PBMC-PLT_D004 D004 ce1 1Dig 1PM 2D 2c 3U 4G 5S (6Oct) 7Rot 8Gp 9Ama 10AsTm 11Azd 14 ce_R ROX N_L N_P N_P N_E N_E NS_E S_E SGp_E ROX CIV ROX 8 5S 12 9Ama RP1_PBMC-PLT Doerrier 2018 Methods Mol Biol The SUIT-001 protocol in combination with SUIT-002 is specially designed to provide a common reference for comparison of respiratory control of mitochondrial preparations in a wide variety of species, tissues and cell types. SUIT-001 gives information of the linear coupling control (L- P- E) with NADH linked-substrates (PM). Moreover, the pathway control in ET state (N, NS, FNS, S and SGp pathways) can be evaluated by using this SUIT protocol. SUIT-001 can be extended with the CIV assay module.
SUIT-002_O2_mt_D005 D005 1mt 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 15 ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX 11 9U 13 11Ama RP2_mt A: Doerrier 2018 Methods Mol Biol The SUIT-002 protocol in combination with SUIT-001 provides a common reference for comparison of respiratory control of mitochondrial preparations in a wide variety of species, tissues and cell types. SUIT-002 is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) can be evaluated by using this SUIT protocol. SUIT-002 can be extended with the CIV assay module.
SUIT-002_O2_pfi_D006 D006 1pfi 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 15 ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX 11 9U 13 11Ama RP2_pfi A: Doerrier 2018 Methods Mol Biol The SUIT-002 protocol in combination with SUIT-001 provides a common reference for comparison of respiratory control of mitochondrial preparations in a wide variety of species, tissues and cell types. SUIT-002 is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) can be evaluated by using this SUIT protocol. SUIT-002 can be extended with the CIV assay module.
SUIT-002_O2_ce-pce_D007 D007 ce1 1Dig 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16 ce_R ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX 12 9U 14 11Ama RP2_pce A: Doerrier 2018 Methods Mol Biol The SUIT-002 protocol in combination with SUIT-001 provides a common reference for comparison of respiratory control of mitochondrial preparations in a wide variety of species, tissues and cell types. SUIT-002 is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) can be evaluated by using this SUIT protocol. SUIT-002 can be extended with the CIV assay module.
SUIT-002_O2_ce-pce-PBMC-PLT_D007a D007a ce1 1Dig 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16 ce_R ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX 12 9U 14 11Ama RP2_PBMC-PLT A: Doerrier 2018 Methods Mol Biol The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-010_O2_ce-pce_D008 D008 ce1 ce2Rot ce3S ce4D 1Dig 1c 6 ce_R ROX ceS_L ceS_P S_P S_P 5 1Dig 2 ce2Rot Digitonin test A: Optimization of digitonin concentration for pce The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-003_O2_ce_D009 D009 ce1 ce2Omy ce3U ce4Ama 4 ce_R ce_L ce_E ROX 3 ce3U 4 ce4Ama CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-004_O2_pfi_D010 D010 1pfi 1PM 2D 2c 3U 4S 5Rot 6Ama 7AsTm 8Azd 10 ROX N_L N_P N_P N_E NS_E S_E ROX CIV ROX 6 4S 8 6Ama RP1-short The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-005_O2_pfi_D011 D011 1pfi 1OctM 2D 2c 3P 4S 5U 6Rot 7Ama 8AsTm 9Azd 11 ROX F(N)_L F(N)_P F(N)_P FN_P FNS_P FNS_E S_E ROX CIV ROX 7 5U 9 7Ama RP2-short A: when malate-anaplerotic activity is zero The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-003_O2_ce_D012 D012 ce1 ce2P ce2Omy ce3U ce4Rot ce5Ama 6 ce_R ce_R ce_L ce_E ROX ROX 4 ce3U 6 ce5Ama CCP-ce_+P A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-003_O2_ce-pce_D013 D013 ce1 ce1P ce2Omy ce3U ce3Glc ce3M ce4Rot ce5S 1Dig 1U 1c 2Ama 3AsTm 4Azd 14 ce_R ce_R ce_L ce_E ce_E ce_E ROX ceS_E S_E S_E S_E ROX CIV ROX 4 ce3U 12 2Ama CCP-ce_+Glc+M+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-008_O2_pfi_D014 D014 1pfi 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 11 ROX N_L N_P N_P N_P NS_P NS_E S_E ROX CIV ROX 7 5U 9 7Ama PM+G+S_OXPHOS+Rot_ET_pfi A: Additivity between the N- and S-pathway in the Q-junction The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-009_O2_mt_D015 D015 1mt 1S 2D 2c 3P 4Rot 5Ama 7 ROX S_L S_P S_P NS_P S_P ROX 5 3P 7 5Ama S_OXPHOS_Rot_O2_mt The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-009_O2_ce-pce_D016 D016 ce1 1Dig 1S 2D 2c 3P 4Rot 5Ama 8 ce_R ROX S_L S_P S_P NS_P S_P ROX 6 3P 8 5Ama S_OXPHOS_Rot_O2_pce The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-003_O2_ce-pce_D018 D018 ce1 ce1P ce2Omy ce3U ce3Glc ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 12 ce_R ce_R ce_L ce_E ce_E ROX ceS_E S_E S_E ROX CIV ROX 4 ce3U 10 2Ama CCP-ce_+Glc+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-003_O2_ce-pce_D020 D020 ce1 ce1P ce2Omy ce3U ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 11 ce_R ce_R ce_L ce_E ROX ceS_E S_E S_E ROX CIV ROX 4 ce3U 9 2Ama CCP-ce_+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-006_O2_mt_D022 D022 1mt 1S(Rot) 2D 2c 3Omy 4U 5Ama 7 ROX S_L S_P S_P S_E ROX 6 4U 7 5Ama CCP-mtprep_Srot A: Coupling control protocol, mtprep The protocols recommended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the LEAK-respiration, we need to inhibit the phosphorylation system which in our protocol is achieved using oligomycin. However, the oligomycin concentration has to be carefully adjusted for different samples and the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET-capacity. SUIT-003 follows a modular approach and can be easily used in combination with the permeability test and/or the assessment of the complex IV capacity.
SUIT-011_O2_pfi_D024 D024 1pfi 1GM 2D 2c 3S 4U 5Rot 6Ama 8 ROX N_L N_P N_P NS_P NS_E S_E ROX 6 4U 8 6Ama GM+S_OXPHOS+Rot_ET:pfi A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control The SUIT-004 protocols are designed to provide a quick assessment of the linear coupling control (L- P- E) with NADH-linked substrates (PM) and the contribution of the S-pathway to the ET state (N, NS, S). SUIT-004 can be extended with the CIV assay module.
SUIT-008_O2_ce-pce_D025 D025 ce1 1Dig 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 12 ce_R ROX N_L N_P N_P N_P NS_P NS_E S_E ROX CIV ROX 8 5U 10 7Ama PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction The SUIT-005 protocols provide information on the F-pathway, the combined FN pathway, and the convergence FNS pathways in the OXPHOS state. FNS comprises the most important pathways in many cell types and thus provides a physiologically relevant estimate of maximum OXPHOS- and ET-capacity. SUIT-005 can be extended with the CIV assay module.
SUIT-008_O2_mt_D026 D026 1mt 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 11 ROX N_L N_P N_P N_P NS_P NS_E S_E ROX CIV ROX 7 5U 9 7Ama PM+G+S_OXPHOS+Rot_ET_mt A: Additivity between the N- and S-pathway in the Q-junction The SUIT-006 protocol is a coupling control protocol for mitochondrial preparations. Depending on the aims of the researcher, different combinations of substrates can be used to assess coupling control (L- P- E or L- P-L(Omy)- E) at a defined electron transfer-pathway state. The use of oligomycin is optional, however, it provides important information when residual and endogenous adenylates are present (which may happen if ATPases are active in the sample). This situation may lead to overestimated LEAK respiration measured in the absence of adenylates - L(n). Therefore, oligomycin can be used to verify whether this occurs and obtain the LEAKstate appropriately. In the DatLab software, SUIT-006 DLP files are currently provided for the categories N(PM) and S. For using this protocol with other substrate/inhibitor combinations, a personalized DLPU can be created. For O2 application with ce-pce, choose SUIT-006_O2_ce-pce_D029 to create the DLPU, for mt, choose SUIT-006 O2 mt D047. For O2 and H2O2 measurements (AmR), choose SUIT-006 AmR mt D048, and for O2 and membrane potential measurements, choose SUIT-006 Fluo mt D034.
SUIT-012_O2_mt_D027 D027 1mt 1PM 2D 2c 3G 4U 5Ama 7 ROX N_L N_P N_P N_P N_E ROX 6 4U 7 5Ama PM+G_OXPHOS_mt A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-003_O2_ce_D028 D028 ce1 ce2Omy ce3U ce4Rot ce5S ce6Ama 6 ce_R ce_L ce_E ROX ceS_E ROX 3 ce3U 6 ce6Ama CCP-ce_+S A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-006_O2_ce-pce_D029 D029 ce1 1Dig 1PM 2D 2c 3Omy 4U 5Ama 8 ce_R ROX N_L N_P N_P N_L N_E ROX 7 4U 8 5Ama CCP-mtprep_pce A: Coupling control protocol, mtprep
SUIT-007_O2_ce-pce_D030 D030 ce1 1Dig 1G 2D 2c 3M 4U 5Ama 8 ce_R ROX N_L N_P N_P N_P N_E ROX 7 4U 8 5Ama Glutamate anaplerosis A: Glutamate anaplerotic pathway
SUIT-020_O2_mt_D032 D032 ROX N_L N_P N_P N_P NS_P S_P S_L S_E ROX 10 ROX N_L N_P N_P N_P NS_P S_P S_L S_E ROX 9 7U 10 8Ama PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_O2_mt_D035 D035 1mt 1GM 2D 2c 3S 4Rot 5Omy 6U 7Ama 9 ROX N_L N_P N_P NS_P S_P S_L S_E ROX 8 6U 9 7Ama OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-003_O2_ce_D037 D037 ce1 ce1Glc ce2Omy ce3U ce4Ama 5 ce_R ce_R ce_E ROX 4 ce3U 5 ce4Ama CCP-ce_+Glc+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D038 D038 ce1 ce2Omy ce3U ce3Glc ce3'U ce4Ama 6 ce_R ce_E ce_E ce_E ROX 3 ce3U 6 ce4Ama CCP-ce_+(Omy)+Glc A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D039 D039 ce1 ce2Omy ce3U ce4Rot ce4Ama 5 ce_R ET ROX ROX 3 ce3U 4 ce4Rot CCP-ce_+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-014_O2_pfi_D042 D042 1pfi 1GM 2D 2c 3P 4S 5U 6Rot 7Ama 9 ROX N_L N_P N_P N_P NS_P NS_E S_E ROX 7 5U 9 7Ama GM+P+S_OXPHOS+Rot_ET_pfi A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
SUIT-015_O2_pti_D043 D043 1pti 1OctM 2D 3G 4P 5S 6U 7Rot 8Ama 9 ROX F(N)_L F(N)_P FN_P FN_P FNS_P FNS_E S_E ROX 7 6U 9 8Ama F+G+P+S_OXPHOS+Rot_ET A: F-pathway in LEAK state and OXPHOS state
SUIT-016_O2_pfi_D044 D044 1pfi 1OctM 2D 3G 4S 5Rot 6Omy 7U 7c 8Ama 10 ROX F(N)_L F(N)_P FN_P FNS_P S_P S_L S_E S_E ROX 8 7U 10 8Ama F+G+S+Rot_OXPHOS+Omy A: F-pathway in LEAK state and OXPHOS state
SUIT-019_O2_pfi_D045 D045 1pfi 1PalM 2D 2c 3Oct 4P 5G 6U 7S 8Rot 9Ama 11 F(N)_L F(N)_P F(N)_P F(N)_P FN_P FN_P FN_E FNS_E S_E ROX 9 7S 11 9Ama Pal+Oct+P+G_OXPHOS+S+Rot_ET A:
SUIT-017_O2_mt _D046 D046 1mt 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 9 ROX F(N)_L F(N)_P F(N)_P FN_P FNS_P FNS_E S_E ROX 7 5U 9 7Ama F+G+S_OXPHOS+Rot_ET_mt A:
SUIT-006_O2_mt_D047 D047 1mt 1PM 2D 2c 3Omy 4U 5Ama 7 ROX N_L N_P N_P N_L N_E ROX 6 4U 7 5Ama CCP-mtprep_PM A: Coupling control protocol, mtprep
SUIT-017_O2_pfi_D049 D049 1pfi 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 9 ROX F(N)_L F(N)_P F(N)_P FN_P FNS_P FNS_E S_E ROX 7 5U 9 7Ama F+G+S_OXPHOS+Rot_ET_pfi A:
SUIT-003_O2_ce_D050 D050 ce1 ce1Snv ce2Omy ce3U ce4Rot ce5Ama 6 ce_R ce_R ce_E ROX ROX 4 ce3U 6 ce5Ama CCP-ce_+Snv+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-022 O2 ce D051 D051 ce1 ce2KCN ce3SHAM 3 ce_R ce_R ROX 3 ce3SHAM 3 ce3SHAM AOX (ce CN+SHAM) A: Determination of the respiration due to the alternative oxidase pathway
SUIT-012_O2_ce-pce_D052 D052 ce1 1Dig 1PM 2D 2c 3G 4U 5Ama 8 ce_R ROX N_L N_P N_P N_P N_E ROX 8 5Ama 7 4U PM+G_OXPHOS_pce A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-023 O2 ce D053 D053 ce1 ce2SHAM ce3KCN 3 ce_R ce_R ROX 3 ce3KCN 3 ce3KCN AOX-ce SHAM+CN A: Determination of respiration through the CIII-CIV pathway
SUIT-018_O2_mt_D054 D054 1mt 1GMS 2D 3Ama 4 ROX NS_L NS_P ROX 3 2D 4 3Ama O2_GMS_mt4
SUIT-024_O2_ce-pce_D056 D056 ce1 1Dig 1PM 2T 2D 3Omy 4Ama 7 ce_R ROX N_L N_P N_P N_L ROX 5 2D 7 4Ama ATPase (PM) A: Determination of the presence of ATPases in mitochondrial preparations.
SUIT-025_O2_mt_D057 D057 1mt 1D 2M.1 03-Oct 3c 4M2 5P 6G 7S 8Rot 9Ama 11 ROX ROX non F(N)_P F(N)_P F(N)_P F(N)_P FN_P FN_P FNS_P S_P ROX 9 7S 11 9Ama OXPHOS (F+M+P+G+S+Rot)
SUIT-003_O2_ce_D060 D060 ce1 ce2Omy ce3U ce4Rot ce5Snv ce6Mnanv ce7Ama 7 ce_R ceP_E ROX ceS_E ROX ROX 3 ce3U 7 ce7Ama CCP-ce_+(Omy)+Snv+Mnanv A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D061 D061 ce1 ce2Omy ce3U ce4Rot ce5DMSO ce6DMSO ce7Ama 7 ce_R ceP_E ROX ROX ROX ROX 3 ce3U 7 ce7Ama CCP-ce_+(Omy)+DMSO+DMSO A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D062 D062 ce1 ce1DMSO ce2Omy ce3U ce4Rot ce5Ama 6 ce_R ce_R ce_E ROX ROX 4 ce3U 6 ce5Ama CCP-ce_+DMSO+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-026_O2_mt_D063 D063 1mt 1S 2Rot 3D 3c 4Ama 6 ROX S_L S_L S_P S_P ROX 4 3D 6 4Ama S+Rot_OXPHOS
SUIT-027_O2_ce-pce_D065 D065 ce1 1Dig 1M.05 2D 3M 3M´ 4P 5G 8 ce_R ROX (N)_P (N)_P N_P N_P 8 5G 2 1Dig Malate anaplerosis A: Malate anaplerotic pathway

Protocol Marks

Protocol name DLP# Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Step 14 Step 15 Step 16 Step 17 Step 18 Step 19 Step 20 # of steps
SUIT-001_O2_ce-pce_D003 D003 ce1 1Dig 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_PBMC-PLT_D004 D004 ce1 1Dig 1PM 2D 2c 3U 4G 5S (6Oct) 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_mt_D001 D001 1mt 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 13
SUIT-001_O2_pfi_D002 D002 1pfi 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 13
SUIT-002_O2_ce-pce_D007 D007 ce1 1Dig 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_ce-pce-PBMC-PLT_D007a D007a ce1 1Dig 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_mt_D005 D005 1mt 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 15
SUIT-002_O2_pfi_D006 D006 1pfi 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 15
SUIT-003_O2_ce_D009 D009 ce1 ce2Omy ce3U ce4Ama 4
SUIT-003_O2_ce_D012 D012 ce1 ce2P ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D028 D028 ce1 ce2Omy ce3U ce4Rot ce5S ce6Ama 6
SUIT-003_O2_ce_D037 D037 ce1 ce1Glc ce2Omy ce3U ce4Ama 5
SUIT-003_O2_ce_D038 D038 ce1 ce2Omy ce3U ce3Glc ce3'U ce4Ama 6
SUIT-003_O2_ce_D039 D039 ce1 ce2Omy ce3U ce4Rot ce4Ama 5
SUIT-003_O2_ce_D050 D050 ce1 ce1Snv ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D060 D060 ce1 ce2Omy ce3U ce4Rot ce5Snv ce6Mnanv ce7Ama 7
SUIT-003_O2_ce_D061 D061 ce1 ce2Omy ce3U ce4Rot ce5DMSO ce6DMSO ce7Ama 7
SUIT-003_O2_ce_D062 D062 ce1 ce1DMSO ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce-pce_D013 D013 ce1 ce1P ce2Omy ce3U ce3Glc ce3M ce4Rot ce5S 1Dig 1U 1c 2Ama 3AsTm 4Azd 14
SUIT-003_O2_ce-pce_D018 D018 ce1 ce1P ce2Omy ce3U ce3Glc ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 12
SUIT-003_O2_ce-pce_D020 D020 ce1 ce1P ce2Omy ce3U ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 11
SUIT-004_O2_pfi_D010 D010 1pfi 1PM 2D 2c 3U 4S 5Rot 6Ama 7AsTm 8Azd 10
SUIT-005_O2_pfi_D011 D011 1pfi 1OctM 2D 2c 3P 4S 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-006_O2_ce-pce_D029 D029 ce1 1Dig 1PM 2D 2c 3Omy 4U 5Ama 8
SUIT-006_O2_mt_D022 D022 1mt 1S(Rot) 2D 2c 3Omy 4U 5Ama 7
SUIT-006_O2_mt_D047 D047 1mt 1PM 2D 2c 3Omy 4U 5Ama 7
SUIT-007_O2_ce-pce_D030 D030 ce1 1Dig 1G 2D 2c 3M 4U 5Ama 8
SUIT-008_O2_ce-pce_D025 D025 ce1 1Dig 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 12
SUIT-008_O2_mt_D026 D026 1mt 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-008_O2_pfi_D014 D014 1pfi 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-009_O2_ce-pce_D016 D016 ce1 1Dig 1S 2D 2c 3P 4Rot 5Ama 8
SUIT-009_O2_mt_D015 D015 1mt 1S 2D 2c 3P 4Rot 5Ama 7
SUIT-010_O2_ce-pce_D008 D008 ce1 ce2Rot ce3S ce4D 1Dig 1c 6
SUIT-011_O2_pfi_D024 D024 1pfi 1GM 2D 2c 3S 4U 5Rot 6Ama 8
SUIT-012_O2_ce-pce_D052 D052 ce1 1Dig 1PM 2D 2c 3G 4U 5Ama 8
SUIT-012_O2_mt_D027 D027 1mt 1PM 2D 2c 3G 4U 5Ama 7
SUIT-014_O2_pfi_D042 D042 1pfi 1GM 2D 2c 3P 4S 5U 6Rot 7Ama 9
SUIT-015_O2_pti_D043 D043 1pti 1OctM 2D 3G 4P 5S 6U 7Rot 8Ama 9
SUIT-016_O2_pfi_D044 D044 1pfi 1OctM 2D 3G 4S 5Rot 6Omy 7U 7c 8Ama 10
SUIT-017_O2_mt _D046 D046 1mt 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 9
SUIT-017_O2_pfi_D049 D049 1pfi 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 9
SUIT-018_O2_mt_D054 D054 1mt 1GMS 2D 3Ama 4
SUIT-019_O2_pfi_D045 D045 1pfi 1PalM 2D 2c 3Oct 4P 5G 6U 7S 8Rot 9Ama 11
SUIT-020_O2_mt_D032 D032 ROX N_L N_P N_P N_P NS_P S_P S_L S_E ROX 10
SUIT-021_O2_mt_D035 D035 1mt 1GM 2D 2c 3S 4Rot 5Omy 6U 7Ama 9
SUIT-022 O2 ce D051 D051 ce1 ce2KCN ce3SHAM 3
SUIT-023 O2 ce D053 D053 ce1 ce2SHAM ce3KCN 3
SUIT-024_O2_ce-pce_D056 D056 ce1 1Dig 1PM 2T 2D 3Omy 4Ama 7
SUIT-025_O2_mt_D057 D057 1mt 1D 2M.1 03-Oct 3c 4M2 5P 6G 7S 8Rot 9Ama 11
SUIT-026_O2_mt_D063 D063 1mt 1S 2Rot 3D 3c 4Ama 6
SUIT-027_O2_ce-pce_D065 D065 ce1 1Dig 1M.05 2D 3M 3M´ 4P 5G 8

Respiratory states

DLP# State 1 State 2 State 3 State 4 State 5 State 6 State 7 State 8 State 9 State 10 State 11 State 12 State 13 State 14 State 15 State 16 State 17 State 18 State 19 State 20
D003 ce_R ROX N_L N_P N_P N_E N_E NS_E FNS_E S_E SGp_E ROX CIV ROX
D004 ce_R ROX N_L N_P N_P N_E N_E NS_E S_E SGp_E ROX CIV ROX
D001 ROX N_L N_P N_P N_E N_E NS_E FNS_E S_E SGp_E ROX CIV ROX
D002 ROX N_L N_P N_P N_E N_E NS_E FNS_E S_E SGp_E ROX CIV ROX
D007 ce_R ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX
D007a ce_R ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX
D005 ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX
D006 ROX ROX F_P F_P F(N)_P FN_P FN_P FNS_P FNSGp_P FNSGp_E SGp_E ROX CIV ROX
D009 ce_R ce_L ce_E ROX
D012 ce_R ce_R ce_L ce_E ROX ROX
D028 ce_R ce_L ce_E ROX ceS_E ROX
D037 ce_R ce_R ce_E ROX
D038 ce_R ce_E ce_E ce_E ROX
D039 ce_R ET ROX ROX
D050 ce_R ce_R ce_E ROX ROX
D060 ce_R ceP_E ROX ceS_E ROX ROX
D061 ce_R ceP_E ROX ROX ROX ROX
D062 ce_R ce_R ce_E ROX ROX
D013 ce_R ce_R ce_L ce_E ce_E ce_E ROX ceS_E S_E S_E S_E ROX CIV ROX
D018 ce_R ce_R ce_L ce_E ce_E ROX ceS_E S_E S_E ROX CIV ROX
D020 ce_R ce_R ce_L ce_E ROX ceS_E S_E S_E ROX CIV ROX
D010 ROX N_L N_P N_P N_E NS_E S_E ROX CIV ROX
D011 ROX F(N)_L F(N)_P F(N)_P FN_P FNS_P FNS_E S_E ROX CIV ROX
D029 ce_R ROX N_L N_P N_P N_L N_E ROX
D022 ROX S_L S_P S_P S_E ROX
D047 ROX N_L N_P N_P N_L N_E ROX
D030 ce_R ROX N_L N_P N_P N_P N_E ROX
D025 ce_R ROX N_L N_P N_P N_P NS_P NS_E S_E ROX CIV ROX
D026 ROX N_L N_P N_P N_P NS_P NS_E S_E ROX CIV ROX
D014 ROX N_L N_P N_P N_P NS_P NS_E S_E ROX CIV ROX
D016 ce_R ROX S_L S_P S_P NS_P S_P ROX
D015 ROX S_L S_P S_P NS_P S_P ROX
D008 ce_R ROX ceS_L ceS_P S_P S_P
D024 ROX N_L N_P N_P NS_P NS_E S_E ROX
D052 ce_R ROX N_L N_P N_P N_P N_E ROX
D027 ROX N_L N_P N_P N_P N_E ROX
D042 ROX N_L N_P N_P N_P NS_P NS_E S_E ROX
D043 ROX F(N)_L F(N)_P FN_P FN_P FNS_P FNS_E S_E ROX
D044 ROX F(N)_L F(N)_P FN_P FNS_P S_P S_L S_E S_E ROX
D046 ROX F(N)_L F(N)_P F(N)_P FN_P FNS_P FNS_E S_E ROX
D049 ROX F(N)_L F(N)_P F(N)_P FN_P FNS_P FNS_E S_E ROX
D054 ROX NS_L NS_P ROX
D045 F(N)_L F(N)_P F(N)_P F(N)_P FN_P FN_P FN_E FNS_E S_E ROX
D032 ROX N_L N_P N_P N_P NS_P S_P S_L S_E ROX
D035 ROX N_L N_P N_P NS_P S_P S_L S_E ROX
D051 ce_R ce_R ROX
D053 ce_R ce_R ROX
D056 ce_R ROX N_L N_P N_P N_L ROX
D057 ROX ROX non F(N)_P F(N)_P F(N)_P F(N)_P FN_P FN_P FNS_P S_P ROX
D063 ROX S_L S_L S_P S_P ROX
D065 ce_R ROX (N)_P (N)_P N_P N_P

Short names and notes

DLP# Short name Notes
D003 RP1_pce Doerrier 2018 Methods Mol Biol
D004 RP1_PBMC-PLT Doerrier 2018 Methods Mol Biol
D001 RP1_mt Doerrier 2018 Methods Mol Biol
D002 RP1_pfi Doerrier 2018 Methods Mol Biol
D007 RP2_pce A: Doerrier 2018 Methods Mol Biol
D007a RP2_PBMC-PLT A: Doerrier 2018 Methods Mol Biol
D005 RP2_mt A: Doerrier 2018 Methods Mol Biol
D006 RP2_pfi A: Doerrier 2018 Methods Mol Biol
D009 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D012 CCP-ce_+P A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D028 CCP-ce_+S A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D037 CCP-ce_+Glc+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D038 CCP-ce_+(Omy)+Glc A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D039 CCP-ce_+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D050 CCP-ce_+Snv+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D060 CCP-ce_+(Omy)+Snv+Mnanv A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D061 CCP-ce_+(Omy)+DMSO+DMSO A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D062 CCP-ce_+DMSO+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D013 CCP-ce_+Glc+M+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D018 CCP-ce_+Glc+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D020 CCP-ce_+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D010 RP1-short
D011 RP2-short A: when malate-anaplerotic activity is zero
D029 CCP-mtprep_pce A: Coupling control protocol, mtprep
D022 CCP-mtprep_Srot A: Coupling control protocol, mtprep
D047 CCP-mtprep_PM A: Coupling control protocol, mtprep
D030 Glutamate anaplerosis A: Glutamate anaplerotic pathway
D025 PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction
D026 PM+G+S_OXPHOS+Rot_ET_mt A: Additivity between the N- and S-pathway in the Q-junction
D014 PM+G+S_OXPHOS+Rot_ET_pfi A: Additivity between the N- and S-pathway in the Q-junction
D016 S_OXPHOS_Rot_O2_pce
D015 S_OXPHOS_Rot_O2_mt
D008 Digitonin test A: Optimization of digitonin concentration for pce
D024 GM+S_OXPHOS+Rot_ET:pfi A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
D052 PM+G_OXPHOS_pce A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
D027 PM+G_OXPHOS_mt A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
D042 GM+P+S_OXPHOS+Rot_ET_pfi A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
D043 F+G+P+S_OXPHOS+Rot_ET A: F-pathway in LEAK state and OXPHOS state
D044 F+G+S+Rot_OXPHOS+Omy A: F-pathway in LEAK state and OXPHOS state
D046 F+G+S_OXPHOS+Rot_ET_mt A:
D049 F+G+S_OXPHOS+Rot_ET_pfi A:
D054 O2_GMS_mt4
D045 Pal+Oct+P+G_OXPHOS+S+Rot_ET A:
D032 PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
D035 OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
D051 AOX (ce CN+SHAM) A: Determination of the respiration due to the alternative oxidase pathway
D053 AOX-ce SHAM+CN A: Determination of respiration through the CIII-CIV pathway
D056 ATPase (PM) A: Determination of the presence of ATPases in mitochondrial preparations.
D057 OXPHOS (F+M+P+G+S+Rot)
D063 S+Rot_OXPHOS
D065 Malate anaplerosis A: Malate anaplerotic pathway

Reference States

DLP# Reference st. Mark Baseline st. Mark
D003 8 5S 12 9Ama
D004 8 5S 12 9Ama
D001 7 5S 11 9Ama
D002 7 5S 11 9Ama
D007 12 9U 14 11Ama
D007a 12 9U 14 11Ama
D005 11 9U 13 11Ama
D006 11 9U 13 11Ama
D009 3 ce3U 4 ce4Ama
D012 4 ce3U 6 ce5Ama
D028 3 ce3U 6 ce6Ama
D037 4 ce3U 5 ce4Ama
D038 3 ce3U 6 ce4Ama
D039 3 ce3U 4 ce4Rot
D050 4 ce3U 6 ce5Ama
D060 3 ce3U 7 ce7Ama
D061 3 ce3U 7 ce7Ama
D062 4 ce3U 6 ce5Ama
D013 4 ce3U 12 2Ama
D018 4 ce3U 10 2Ama
D020 4 ce3U 9 2Ama
D010 6 4S 8 6Ama
D011 7 5U 9 7Ama
D029 7 4U 8 5Ama
D022 6 4U 7 5Ama
D047 6 4U 7 5Ama
D030 7 4U 8 5Ama
D025 8 5U 10 7Ama
D026 7 5U 9 7Ama
D014 7 5U 9 7Ama
D016 6 3P 8 5Ama
D015 5 3P 7 5Ama
D008 5 1Dig 2 ce2Rot
D024 6 4U 8 6Ama
D052 8 5Ama 7 4U
D027 6 4U 7 5Ama
D042 7 5U 9 7Ama
D043 7 6U 9 8Ama
D044 8 7U 10 8Ama
D046 7 5U 9 7Ama
D049 7 5U 9 7Ama
D054 3 2D 4 3Ama
D045 9 7S 11 9Ama
D032 9 7U 10 8Ama
D035 8 6U 9 7Ama
D051 1 ce1 3 ce3SHAM
D053 1 ce1 3 ce3KCN
D056 5 2D 7 4Ama
D057 9 7S 11 9Ama
D063 4 3D 6 4Ama
D065 8 5G 2 1Dig
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