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Excel templ: Protocols

From Bioblast

Attention! Attention!

Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.

This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.

Protocol Marks

Protocol name DLP# Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Step 14 Step 15 Step 16 Step 17 Step 18 Step 19 Step 20 # of steps
SUIT-001_O2_ce-pce_D003 D003 ce1 1Dig 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_PBMC-PLT_D004 D004 ce1 1Dig 1PM 2D 2c 3U 4G 5S (6Oct) 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_mt_D001 D001 1mt 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 13
SUIT-001_O2_pfi_D002 D002 1pfi 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 13
SUIT-002_O2_ce-pce_D007 D007 ce1 1Dig 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_ce-pce-PBMC-PLT_D007a D007a ce1 1Dig 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_mt_D005 D005 1mt 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 15
SUIT-002_O2_pfi_D006 D006 1pfi 1D 2M.1 3Oct 3c 4M2 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 15
SUIT-003_O2_ce_D009 D009 ce1 ce2Omy ce3U ce4Ama 4
SUIT-003_O2_ce_D012 D012 ce1 ce2P ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D028 D028 ce1 ce2Omy ce3U ce4Rot ce5S ce6Ama 6
SUIT-003_O2_ce_D037 D037 ce1 ce1Glc ce2Omy ce3U ce4Ama 5
SUIT-003_O2_ce_D038 D038 ce1 ce2Omy ce3U ce3Glc ce3'U ce4Ama 6
SUIT-003_O2_ce_D039 D039 ce1 ce2Omy ce3U ce4Rot ce4Ama 5
SUIT-003_O2_ce_D050 D050 ce1 ce1Snv ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D060 D060 ce1 ce2Omy ce3U ce4Rot ce5Snv ce6Mnanv ce7Ama 7
SUIT-003_O2_ce_D061 D061 ce1 ce2Omy ce3U ce4Rot ce5DMSO ce6DMSO ce7Ama 7
SUIT-003_O2_ce_D062 D062 ce1 ce1DMSO ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce-pce_D013 D013 ce1 ce1P ce2Omy ce3U ce3Glc ce3M ce4Rot ce5S 1Dig 1U 1c 2Ama 3AsTm 4Azd 14
SUIT-003_O2_ce-pce_D018 D018 ce1 ce1P ce2Omy ce3U ce3Glc ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 12
SUIT-003_O2_ce-pce_D020 D020 ce1 ce1P ce2Omy ce3U ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 11
SUIT-004_O2_pfi_D010 D010 1pfi 1PM 2D 2c 3U 4S 5Rot 6Ama 7AsTm 8Azd 10
SUIT-005_O2_pfi_D011 D011 1pfi 1OctM 2D 2c 3P 4S 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-006_O2_ce-pce_D029 D029 ce1 1Dig 1PM 2D 2c 3Omy 4U 5Ama 8
SUIT-006_O2_mt_D022 D022 1mt 1S(Rot) 2D 2c 3Omy 4U 5Ama 7
SUIT-006_O2_mt_D047 D047 1mt 1PM 2D 2c 3Omy 4U 5Ama 7
SUIT-007_O2_ce-pce_D030 D030 ce1 1Dig 1G 2D 2c 3M 4U 5Ama 8
SUIT-008_O2_ce-pce_D025 D025 ce1 1Dig 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 12
SUIT-008_O2_mt_D026 D026 1mt 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-008_O2_pfi_D014 D014 1pfi 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-009_O2_ce-pce_D016 D016 ce1 1Dig 1S 2D 2c 3P 4Rot 5Ama 8
SUIT-009_O2_mt_D015 D015 1mt 1S 2D 2c 3P 4Rot 5Ama 7
SUIT-010_O2_ce-pce_D008 D008 ce1 ce2Rot ce3S ce4D 1Dig 1c 6
SUIT-011_O2_pfi_D024 D024 1pfi 1GM 2D 2c 3S 4U 5Rot 6Ama 8
SUIT-012_O2_ce-pce_D052 D052 ce1 1Dig 1PM 2D 2c 3G 4U 5Ama 8
SUIT-012_O2_mt_D027 D027 1mt 1PM 2D 2c 3G 4U 5Ama 7
SUIT-014_O2_pfi_D042 D042 1pfi 1GM 2D 2c 3P 4S 5U 6Rot 7Ama 9
SUIT-015_O2_pti_D043 D043 1pti 1OctM 2D 3G 4P 5S 6U 7Rot 8Ama 9
SUIT-016_O2_pfi_D044 D044 1pfi 1OctM 2D 3G 4S 5Rot 6Omy 7U 7c 8Ama 10
SUIT-017_O2_mt _D046 D046 1mt 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 9
SUIT-017_O2_pfi_D049 D049 1pfi 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 9
SUIT-018_O2_mt_D054 D054 1mt 1GMS 2D 3Ama 4
SUIT-019_O2_pfi_D045 D045 1pfi 1PalM 2D 2c 3Oct 4P 5G 6U 7S 8Rot 9Ama 11
SUIT-020_O2_mt_D032 D032 ROX N_L N_P N_P N_P NS_P S_P S_L S_E ROX 10
SUIT-021_O2_mt_D035 D035 1mt 1GM 2D 2c 3S 4Rot 5Omy 6U 7Ama 9
SUIT-022 O2 ce D051 D051 ce1 ce2KCN ce3SHAM 3
SUIT-023 O2 ce D053 D053 ce1 ce2SHAM ce3KCN 3
SUIT-024_O2_ce-pce_D056 D056 ce1 1Dig 1PM 2T 2D 3Omy 4Ama 7
SUIT-025_O2_mt_D057 D057 1mt 1D 2M.1 03-Oct 3c 4M2 5P 6G 7S 8Rot 9Ama 11
SUIT-026_O2_mt_D063 D063 1mt 1S 2Rot 3D 3c 4Ama 6
SUIT-027_O2_ce-pce_D065 D065 ce1 1Dig 1M.05 2D 3M 3M´ 4P 5G 8

Respiratory states

Short names and notes

DLP# Short name Notes
D003 RP1_pce Doerrier 2018 Methods Mol Biol
D004 RP1_PBMC-PLT Doerrier 2018 Methods Mol Biol
D001 RP1_mt Doerrier 2018 Methods Mol Biol
D002 RP1_pfi Doerrier 2018 Methods Mol Biol
D007 RP2_pce A: Doerrier 2018 Methods Mol Biol
D007a RP2_PBMC-PLT A: Doerrier 2018 Methods Mol Biol
D005 RP2_mt A: Doerrier 2018 Methods Mol Biol
D006 RP2_pfi A: Doerrier 2018 Methods Mol Biol
D009 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D012 CCP-ce_+P A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D028 CCP-ce_+S A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D037 CCP-ce_+Glc+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D038 CCP-ce_+(Omy)+Glc A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D039 CCP-ce_+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D050 CCP-ce_+Snv+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D060 CCP-ce_+(Omy)+Snv+Mnanv A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D061 CCP-ce_+(Omy)+DMSO+DMSO A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D062 CCP-ce_+DMSO+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D013 CCP-ce_+Glc+M+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D018 CCP-ce_+Glc+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D020 CCP-ce_+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
D010 RP1-short
D011 RP2-short A: when malate-anaplerotic activity is zero
D029 CCP-mtprep_pce A: Coupling control protocol, mtprep
D022 CCP-mtprep_Srot A: Coupling control protocol, mtprep
D047 CCP-mtprep_PM A: Coupling control protocol, mtprep
D030 Glutamate anaplerosis A: Glutamate anaplerotic pathway
D025 PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction
D026 PM+G+S_OXPHOS+Rot_ET_mt A: Additivity between the N- and S-pathway in the Q-junction
D014 PM+G+S_OXPHOS+Rot_ET_pfi A: Additivity between the N- and S-pathway in the Q-junction
D016 S_OXPHOS_Rot_O2_pce
D015 S_OXPHOS_Rot_O2_mt
D008 Digitonin test A: Optimization of digitonin concentration for pce
D024 GM+S_OXPHOS+Rot_ET:pfi A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
D052 PM+G_OXPHOS_pce A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
D027 PM+G_OXPHOS_mt A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
D042 GM+P+S_OXPHOS+Rot_ET_pfi A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
D043 F+G+P+S_OXPHOS+Rot_ET A: F-pathway in LEAK state and OXPHOS state
D044 F+G+S+Rot_OXPHOS+Omy A: F-pathway in LEAK state and OXPHOS state
D046 F+G+S_OXPHOS+Rot_ET_mt A:
D049 F+G+S_OXPHOS+Rot_ET_pfi A:
D054 O2_GMS_mt4
D045 Pal+Oct+P+G_OXPHOS+S+Rot_ET A:
D032 PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
D035 OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
D051 AOX (ce CN+SHAM) A: Determination of the respiration due to the alternative oxidase pathway
D053 AOX-ce SHAM+CN A: Determination of respiration through the CIII-CIV pathway
D056 ATPase (PM) A: Determination of the presence of ATPases in mitochondrial preparations.
D057 OXPHOS (F+M+P+G+S+Rot)
D063 S+Rot_OXPHOS
D065 Malate anaplerosis A: Malate anaplerotic pathway

Reference States

DLP# Reference st. Mark Baseline st. Mark
D003 8 5S 12 9Ama
D004 8 5S 12 9Ama
D001 7 5S 11 9Ama
D002 7 5S 11 9Ama
D007 12 9U 14 11Ama
D007a 12 9U 14 11Ama
D005 11 9U 13 11Ama
D006 11 9U 13 11Ama
D009 3 ce3U 4 ce4Ama
D012 4 ce3U 6 ce5Ama
D028 3 ce3U 6 ce6Ama
D037 4 ce3U 5 ce4Ama
D038 3 ce3U 6 ce4Ama
D039 3 ce3U 4 ce4Rot
D050 4 ce3U 6 ce5Ama
D060 3 ce3U 7 ce7Ama
D061 3 ce3U 7 ce7Ama
D062 4 ce3U 6 ce5Ama
D013 4 ce3U 12 2Ama
D018 4 ce3U 10 2Ama
D020 4 ce3U 9 2Ama
D010 6 4S 8 6Ama
D011 7 5U 9 7Ama
D029 7 4U 8 5Ama
D022 6 4U 7 5Ama
D047 6 4U 7 5Ama
D030 7 4U 8 5Ama
D025 8 5U 10 7Ama
D026 7 5U 9 7Ama
D014 7 5U 9 7Ama
D016 6 3P 8 5Ama
D015 5 3P 7 5Ama
D008 5 1Dig 2 ce2Rot
D024 6 4U 8 6Ama
D052 8 5Ama 7 4U
D027 6 4U 7 5Ama
D042 7 5U 9 7Ama
D043 7 6U 9 8Ama
D044 8 7U 10 8Ama
D046 7 5U 9 7Ama
D049 7 5U 9 7Ama
D054 3 2D 4 3Ama
D045 9 7S 11 9Ama
D032 9 7U 10 8Ama
D035 8 6U 9 7Ama
D051 3 ce3SHAM 3 ce3SHAM
D053 3 ce3KCN 3 ce3KCN
D056 5 2D 7 4Ama
D057 9 7S 11 9Ama
D063 4 3D 6 4Ama
D065 8 5G 2 1Dig