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Excel templ: Protocols

From Bioblast

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Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.

This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.

Protocol name D number Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Step 14 Step 15 Step 16 Step 17 Step 18 Step 19 Step 20 Number of steps
SUIT-001_O2_ce-pce_D003 D003 ce1 1Dig 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_PBMC-PLT_D004 D004 ce1 1Dig 1PM 2D 2c 3U 4G 5S (6Oct) 7Rot 8Gp 9Ama 10AsTm 11Azd 14
SUIT-001_O2_mt_D001 D001 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 12
SUIT-001_O2_pfi_D002 D002 1PM 2D 2c 3U 4G 5S 6Oct 7Rot 8Gp 9Ama 10AsTm 11Azd 12
SUIT-002_O2_ce-pce_D007 D007 ce1 1Dig 1D 2M 4Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_ce-pce-PBMC-PLT_D007a D007a ce1 1Dig 1D 2M 4Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 16
SUIT-002_O2_mt_D005 D005 1D 2M 3Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 14
SUIT-002_O2_pfi_D006 D006 1D 2M 3Oct 3c 4M 5P 6G 7S 8Gp 9U 10Rot 11Ama 12AsTm 13Azd 14
SUIT-003_AmR_ce_D017 D017 ce1 ce2P ce3Omy ce4U ce5Rot ce6S ce7Ama 7
SUIT-003_AmR_ce_D058 D058 ce1 ce1SOD ce1HRP ce1AmR ce2Omy ce3U ce4Rot ce5Ama 8
SUIT-003_AmR_ce_D059 D059 ce1 ce1H2O ce1MiR05 ce1DMSO ce2Omy ce3U ce4Rot ce5Ama 8
SUIT-003_O2_ce_D009 D009 ce1 ce2Omy ce3U ce4Rot ce5Ama 5
SUIT-003_O2_ce_D012 D012 ce1 ce1P ce2Omy ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D028 D028 ce1 ce2Omy ce3U ce4Rot ce5S ce6Ama 6
SUIT-003_O2_ce_D037 D037 ce1 ce1Glc (ce2Omy) ce3U ce4Ama 5
SUIT-003_O2_ce_D038 D038 ce1 (ce2Omy) ce3U ce3Glc ce3'U ce4Ama 6
SUIT-003_O2_ce_D039 D039 ce1 (ce2Omy) ce3U ce4Rot ce5Ama 5
SUIT-003_O2_ce_D050 D050 ce1 ce1Snv (ce2Omy) ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce_D060 D060 ce1 (ce2Omy) ce3U ce4Rot ce5Snv ce6Mnanv ce7Ama 7
SUIT-003_O2_ce_D061 D061 ce1 (ce2Omy) ce3U ce4Rot ce5DMSO ce6DMSO ce7Ama 7
SUIT-003_O2_ce_D062 D062 ce1 ce1DMSO (ce2Omy) ce3U ce4Rot ce5Ama 6
SUIT-003_O2_ce-pce_D013 D013 ce1 ce1P ce2Omy ce3U ce3Glc ce3M ce4Rot ce5S 1Dig 1U 1c 2Ama 3AsTm 4Azd 14
SUIT-003_O2_ce-pce_D018 D018 ce1 ce1P ce2Omy ce3U ce3Glc ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 12
SUIT-003_O2_ce-pce_D020 D020 ce1 ce1P ce2Omy ce3U ce4Rot ce5S 1Dig 1c 2Ama 3AsTm 4Azd 11
SUIT-004_O2_pfi_D010 D010 1PM 2D 2c 3U 4S 5Rot 6Ama 7AsTm 8Azd 9
SUIT-005_O2_pfi_D011 D011 1OctM 2D 3P 4S 5U 6Rot 7Ama 8AsTm 9Azd 9
SUIT-006_AmR_mt_D048 D048 1PM 2D 3Omy 4U 5Ama 5
SUIT-006_Fluo_mt_D034 D034 1PM 2D 3Omy 4U 5Ama 5
SUIT-006_MgG_mt_D055 D055 1PM 2D 3Cat 4U 5Ama 5
SUIT-006_O2_ce-pce_D029 D029 ce1 1Dig 1PM 2D 2c 3Omy 4U 5Ama 8
SUIT-006_O2_mt_D022 D022 1SRot 2D 2c (3Omy) 4U 5Ama 6
SUIT-006_O2_mt_D047 D047 1PM 2D 2c 3Omy 4U 5Ama 6
SUIT-007_O2_ce-pce_D030 D030 ce1 1Dig 1G 2D 2c 3M 4U 5Ama 8
SUIT-008_O2_ce-pce_D025 D025 ce1 1Dig 1PM 2D 2c 3G 4S 5U 6Rot 7Ama 8AsTm 9Azd 12
SUIT-008_O2_mt_D026 D026 1PM 2D 2c 3G 4S 4D 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-008_O2_pfi_D014 D014 1PM 2D 2c 3G 4S 4D 5U 6Rot 7Ama 8AsTm 9Azd 11
SUIT-009_AmR_ce-pce_D019 D019 ce1 1Dig 1S 2D 3P 4Rot 5Ama 7
SUIT-009_AmR_mt_D021 D021 1S 2D 3P 4Rot 5Ama 5
SUIT-009_O2_ce-pce_D016 D016 ce1 1Dig 1S 2D 3P 4Rot 5Ama 7
SUIT-009_O2_mt_D015 D015 1S 2D 3P 4Rot 5Ama 5
SUIT-010_O2_ce-pce_D008 D008 ce1 ce2Rot ce3S ce4D 1Dig 1c 6
SUIT-011_O2_pfi_D024 D024 1GM 2D 2c 3S 4U 5Rot 6Ama 7
SUIT-012_O2_ce-pce_D052 D052 ce1 1Dig 1PM 2D 2c 3G 4U 5Ama 8
SUIT-012_O2_mt_D027 D027 1PM 2D 2c 3G 4U 5Ama 6
SUIT-013_AmR_ce_D023 D023 ce1 1
SUIT-014_O2_pfi_D042 D042 1GM 2D 3P 4S 5U 6Rot 7Ama 7
SUIT-015_O2_pti_D043 D043 1OctM 2D 3G 4P 5S 6U 7Rot 8Ama 8
SUIT-016_O2_pfi_D044 D044 1OctM 2D 3G 4S 5Rot 6Omy 7U 8Ama 8
SUIT-017_O2_mt _D046 D046 1OctM 2D 3G 3c 4S 5U 6Rot 7Ama 8
SUIT-017_O2_pfi_D049 D049 1OctM 2D 2c 3G 4S 5U 6Rot 7Ama 8
SUIT-018_AmR_mt_D031 D031 1GMS 2D 3Ama 3
SUIT-018_AmR_mt_D040 D040 1GMS 2D 3Ama 3
SUIT-018_AmR_mt_D041 D041 1GMS 2D 3Ama 3
SUIT-018_O2_mt_D054 D054 1GMS 2D 3Ama 3
SUIT-019_O2_pfi_D045 D045 1PalM 2D 2c 03-Oct 4P 5G 6U 7S 8Rot 9Ama 10
SUIT-020_Fluo_mt_D033 D033 1PM 2D 3G 4S 5Rot 6Omy 7U 8Ama 8
SUIT-020_O2_mt_D032 D032 1PM 2D 2c 3G 4S 5Rot 6Omy 7U 8Ama 9
SUIT-021_Fluo_mt_D036 D036 1GM 2D 3S 4Rot 5Omy 6U 7Ama 7
SUIT-021_O2_mt_D035 D035 1GM 2D 2c 3S 4Rot 5Omy 6U 7Ama 8
SUIT-022 O2 ce D051 D051 ce1 ce2KCN ce3SHAM 3
SUIT-023 O2 ce D053 D053 ce1 ce2SHAM ce3KCN 3
SUIT-024_O2_ce-pce_D056 D056 ce1 1Dig 1PM 2T 2D 3Omy 4Ama 7
SUIT-025_O2_mt_D057 D057 1D 2M 03-Oct 3c 4M 5P 6G 7S 8Rot 9Ama 10
SUIT-026_AmR_mt_D064 D064 1S 2Rot 3D 4Ama 4
SUIT-026_O2_mt_D063 D063 1S 2Rot 3D 3c 4Ama 5
SUIT-027_O2_ce-pce_D065 D065 ce1 1Dig 1M 2D 3M 4P 5G 7
SUIT-006_Fluo_mt_D034_chemical background D034_chemical backgr. 0
SUIT-020_Fluo_mt_D033_chemical_background D033_chemical backgr. 0
SUIT-021_Fluo_mt_D036_chemical background D036_chemical backgr. 0


Protocol name D number Short name Notes
SUIT-001_O2_ce-pce_D003 D003 RP1_pce Doerrier 2018 Methods Mol Biol
SUIT-001_O2_PBMC-PLT_D004 D004 RP1_PBMC-PLT Doerrier 2018 Methods Mol Biol
SUIT-001_O2_mt_D001 D001 RP1_mt Doerrier 2018 Methods Mol Biol
SUIT-001_O2_pfi_D002 D002 RP1_pfi Doerrier 2018 Methods Mol Biol
SUIT-002_O2_ce-pce_D007 D007 RP2_pce A: Doerrier 2018 Methods Mol Biol
SUIT-002_O2_ce-pce-PBMC-PLT_D007a D007a RP2_PBMC-PLT A: Doerrier 2018 Methods Mol Biol
SUIT-002_O2_mt_D005 D005 RP2_mt A: Doerrier 2018 Methods Mol Biol
SUIT-002_O2_pfi_D006 D006 RP2_pfi A: Doerrier 2018 Methods Mol Biol
SUIT-003_AmR_ce_D017 D017 CCP-ce_+P+S A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_AmR_ce_D058 D058 CCP-ce_+AmR A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_AmR_ce_D059 D059 CCP-ce_+DMSO A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D009 D009 CCP-ce A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D012 D012 CCP-ce_+P A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D028 D028 CCP-ce_+S A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D037 D037 CCP-ce_+Glc+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D038 D038 CCP-ce_+(Omy)+Glc A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D039 D039 CCP-ce_+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D050 D050 CCP-ce_+Snv+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D060 D060 CCP-ce_+(Omy)+Snv+Mnanv A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D061 D061 CCP-ce_+(Omy)+DMSO+DMSO A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce_D062 D062 CCP-ce_+DMSO+(Omy) A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce-pce_D013 D013 CCP-ce_+Glc+M+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce-pce_D018 D018 CCP-ce_+Glc+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-003_O2_ce-pce_D020 D020 CCP-ce_+viability+CIV A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf
SUIT-004_O2_pfi_D010 D010 RP1-short
SUIT-005_O2_pfi_D011 D011 RP2-short A: when malate-anaplerotic activity is zero
SUIT-006_AmR_mt_D048 D048 CCP-mtprep_AmR A: Coupling control protocol, mtprep
SUIT-006_Fluo_mt_D034 D034 CCP-mtprep_Fluo A: Coupling control protocol, mtprep
SUIT-006_MgG_mt_D055 D055 CCP-mtprep_MgG A: Coupling control protocol, mtprep
SUIT-006_O2_ce-pce_D029 D029 CCP-mtprep_pce A: Coupling control protocol, mtprep
SUIT-006_O2_mt_D022 D022 CCP-mtprep_Srot A: Coupling control protocol, mtprep
SUIT-006_O2_mt_D047 D047 CCP-mtprep_PM A: Coupling control protocol, mtprep
SUIT-007_O2_ce-pce_D030 D030 Glutamate anaplerosis A: Glutamate anaplerotic pathway
SUIT-008_O2_ce-pce_D025 D025 PM+G+S_OXPHOS+Rot_ET A: Additivity between the N- and S-pathway in the Q-junction
SUIT-008_O2_mt_D026 D026 PM+G+S_OXPHOS+Rot_ET_mt A: Additivity between the N- and S-pathway in the Q-junction
SUIT-008_O2_pfi_D014 D014 PM+G+S_OXPHOS+Rot_ET_pfi A: Additivity between the N- and S-pathway in the Q-junction
SUIT-009_AmR_ce-pce_D019 D019 S_OXPHOS_Rot_AmR_pce
SUIT-009_AmR_mt_D021 D021 S_OXPHOS_Rot_AmR_mt
SUIT-009_O2_ce-pce_D016 D016 S_OXPHOS_Rot_O2_pce
SUIT-009_O2_mt_D015 D015 S_OXPHOS_Rot_O2_mt
SUIT-010_O2_ce-pce_D008 D008 Digitonin test A: Optimization of digitonin concentration for pce
SUIT-011_O2_pfi_D024 D024 GM+S_OXPHOS+Rot_ET:pfi A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control
SUIT-012_O2_ce-pce_D052 D052 PM+G_OXPHOS_pce A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-012_O2_mt_D027 D027 PM+G_OXPHOS_mt A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM)
SUIT-013_AmR_ce_D023 D023 AmR_ROUTINE
SUIT-014_O2_pfi_D042 D042 GM+P+S_OXPHOS+Rot_ET_pfi A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration
SUIT-015_O2_pti_D043 D043 F+G+P+S_OXPHOS+Rot_ET A: F-pathway in LEAK state and OXPHOS state
SUIT-016_O2_pfi_D044 D044 F+G+S+Rot_OXPHOS+Omy A: F-pathway in LEAK state and OXPHOS state
SUIT-017_O2_mt _D046 D046 F+G+S_OXPHOS+Rot_ET_mt A:
SUIT-017_O2_pfi_D049 D049 F+G+S_OXPHOS+Rot_ET_pfi A:
SUIT-018_AmR_mt_D031 D031 AmR_GMS_mt1
SUIT-018_AmR_mt_D040 D040 AmR_GMS_mt2
SUIT-018_AmR_mt_D041 D041 AmR_GMS_mt3
SUIT-018_O2_mt_D054 D054 O2_GMS_mt4
SUIT-019_O2_pfi_D045 D045 Pal+Oct+P+G_OXPHOS+S+Rot_ET A:
SUIT-020_Fluo_mt_D033 D033 PM+G+S+Rot_OXPHOS+Omy_Fluo A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-020_O2_mt_D032 D032 PM+G+S+Rot_OXPHOS+Omy A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_Fluo_mt_D036 D036 OXPHOS (GM+S+Rot+Omy)_Fluo A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_O2_mt_D035 D035 OXPHOS (GM+S+Rot+Omy) A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-022 O2 ce D051 D051 AOX (ce CN+SHAM) A: Determination of the respiration due to the alternative oxidase pathway
SUIT-023 O2 ce D053 D053 AOX-ce SHAM+CN A: Determination of respiration through the CIII-CIV pathway
SUIT-024_O2_ce-pce_D056 D056 ATPase (PM) A: Determination of the presence of ATPases in mitochondrial preparations.
SUIT-025_O2_mt_D057 D057 OXPHOS (F+M+P+G+S+Rot)
SUIT-026_AmR_mt_D064 D064 S+Rot_OXPHOS_AmR
SUIT-026_O2_mt_D063 D063 S+Rot_OXPHOS
SUIT-027_O2_ce-pce_D065 D065 Malate anaplerosis A: Malate anaplerotic pathway
SUIT-006_Fluo_mt_D034_chemical background D034_chemical backgr. CCP-mtprep_chemical background A: Coupling control protocol, mtprep
SUIT-020_Fluo_mt_D033_chemical_background D033_chemical backgr. PM+G+S+Rot_OXPHOS+Omy_chemical background A: simultaneous determination of O2 flux and mt-membrane potential
SUIT-021_Fluo_mt_D036_chemical background D036_chemical backgr. OXPHOS (GM+S+Rot+Omy)_chemical background A: simultaneous determination of O2 flux and mt-membrane potential